Protein detection and localization of the non-selective cation channel TRPC6 in the human heart

Jacobs, Tobias; Abdinghoff, Jan; Tschernig, Thomas

doi:10.1016/j.ejphar.2022.174972

Cited by 3 publications

(5 citation statements)

References 47 publications

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“…The analyzed areas can be sampled reproducibly based on anatomic landmarks. This is the basis for side-to-side comparability of the specimens missing in the earlier study [ 8 ]. Additionally, a comprehensive macroscopic and microscopic pathologic analysis based on organ autopsy and histopathological assessment was provided in the present study and is lacking in the so far published study on TRPC6-protein in the human heart [ 8 ].…”

Section: Discussionmentioning

confidence: 93%

“…This is the basis for side-to-side comparability of the specimens missing in the earlier study [ 8 ]. Additionally, a comprehensive macroscopic and microscopic pathologic analysis based on organ autopsy and histopathological assessment was provided in the present study and is lacking in the so far published study on TRPC6-protein in the human heart [ 8 ]. The need for such a background is highlighted by the fact that the macroscopic and microscopic findings in this study differed from the information provided by the death certificates (compare Supplement B ).…”

Section: Discussionmentioning

confidence: 93%

“…Concerning the human heart, initial studies on TRPC6-protein expression in cardiac cells (e.g., the valvular interstitium [ 10 ]) or tissues [ 8 ] have been published. In accordance with the previous study of TRPC6-protein in human cardiac tissue by Jacobs and colleagues [ 8 ], the present study observed a widespread TRPC6-protein distribution within the anatomical regions analyzed, and in addition to those findings the present study has included an analysis of the TRPC6-protein distribution in anatomical areas of distinct clinical relevance in the human heart. The analyzed areas can be sampled reproducibly based on anatomic landmarks.…”

Section: Discussionmentioning

confidence: 99%

“…A systematic literature search (last database query 8 October 2022; 12:41 pm; database: —National Library of Medicine) identified overall 105 publications regarding TRPC6 and the heart (3 found by “(((location TRPC6) AND (heart)) AND (human)” and 102 identified by “((TRPC6) AND (heart)) AND (human))”). Within these studies, only one article reporting on the distribution of TRPC6-protein within transmural samples of the human heart was identified [ 8 ]. Most of the others presented data on animal models (e.g., rat model [ 9 ]) or distinct cells (e.g., interstitial cells of human heart valves [ 10 ]).…”

Section: Introductionmentioning

confidence: 99%

“…Most of the others presented data on animal models (e.g., rat model [ 9 ]) or distinct cells (e.g., interstitial cells of human heart valves [ 10 ]). The lone study on the TRPC6-protein distribution in the human heart immunohistochemically analyzed TRPC6-protein distribution in the cardiac layers and in rough anatomic parts (i.e., ventricle or atrium) without reporting a standardization of the sampling sites [ 8 ]. As such, these samples lack comparability, and a systematic mapping of TRPC6-protein in the human heart has not been reported so far.…”

Section: Introductionmentioning

confidence: 99%

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Elderly with Varying Extents of Cardiac Disease Show Interindividual Fluctuating Myocardial TRPC6-Immunoreactivity

Federspiel

Gartner

Lipp

et al. 2023

JCDD

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Both particular myocardial locations in the human heart and the canonical transient receptor potential 6 (TRPC6) cation channel have been linked with cardiac pathophysiologies. Thus, the present study mapped TRPC6-protein distribution in select anatomic locations associated with cardiac disease in the context of an orienting pathological assessment. Specimens were obtained from 5 body donors (4 formalin fixation, 1 nitrite pickling salt-ethanol-polyethylene glycol (NEP) fixation; median age 81 years; 2 females) and procured for basic histological stains and TRPC6-immunohistochemistry. The latter was analyzed descriptively regarding distribution and intensity of positive signals. The percentage of positively labelled myocardium was also determined (optical threshold method). Exclusively exploratory statistical analyses were performed. TRPC6-protein was distributed widespread and homogenously within each analyzed sample. TRPC6-immunoreactive myocardial area was comparable regarding the different anatomic regions and sex. A significantly larger area of TRPC6-immunoreactive myocardium was found in the NEP-fixed donor compared to the formalin fixed donors. Two donors with more severe heart disease showed smaller areas of myocardial TRPC6-immunoreactivity overall compared to the other 3 donors. In summary, in the elderly, TRPC6-protein is widely and homogenously distributed, and severe cardiac disease might be associated with less TRPC6-immunoreactive myocardial area. The tissue fixation method represents a potential confounder.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Elderly with Varying Extents of Cardiac Disease Show Interindividual Fluctuating Myocardial TRPC6-Immunoreactivity

Federspiel

Gartner

Lipp

et al. 2023

JCDD

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TRPC6 promotes daunorubicin-induced mitochondrial fission and cell death in rat cardiomyocytes with the involvement of ERK1/2-DRP1 activation

Wang

Jiang

et al. 2023

Toxicology and Applied Pharmacology

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TRPC6 is ubiquitously present in lymphatic tissues: A study using samples from body donors

Daum,

Flockerzi,

Bozzato

et al. 2024

Med Int

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Transient receptor potential canonical channel 6 (TRPC6) is a non-selective cation channel that is activated by diacylglycerol. It belongs to the TRP superfamily, is expressed in numerous tissues and has been shown to be associated with diseases, such as focal segmental glomerulosclerosis, idiopathic pulmonary arterial hypertension and cardiac hypertrophy. The investigation of the channel in human lymphoid tissues has thus far been limited to mRNA analysis or the western blotting of isolated lymphoid cell lines. The present study aimed to detect the channel in human lymphoid tissue using immunohistochemistry. For this purpose, lymphatic tissues were obtained from body donors. The lymphatic organs analyzed included the lymph nodes, spleen, palatine tonsil, gut-associated lymphoid tissues (ileum and vermiform appendix) and thymus. A total of 102 samples were obtained and processed for hematoxylin and eosin (H&E) staining. The H&E staining method was employed to identify five samples with good morphology. In total, three samples of the palatine tonsil of patients were included. Immunostaining was carried out using a knockout-validated anti-TRPC6 antibody. As shown by the results, using immunohistochemical staining, the presence of TRPC6 was confirmed in all the analyzed lymphatic tissue samples. Lymphocytes in lymph nodes, spleen, palatine tonsil, thymus, and gut-associated lymphatic tissues in ileum and vermiform appendix exhibited a positive staining signal. The follicle-associated epithelium of the palatine tonsil, ileum and appendix also demonstrated staining. Vessels of the lymphatic organs, particularly the trabecular arteries of the spleen, the submucosal vessels of the appendix and ileum, as well as the high endothelial venules in the palatine tonsils and lymphatic vessels of the lymph nodes expressed TRPC6 protein. TRPC6 in follicles may be involved in the immune response. TRPC6 in high endothelial venules suggests a role in leukocyte migration. The role of TRPC6 and other channels of the TRP family in lymphatic organs warrant further investigations to elucidate whether TRP channels are a pharmacological target.

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Protein detection and localization of the non-selective cation channel TRPC6 in the human heart

Cited by 3 publications

References 47 publications

Elderly with Varying Extents of Cardiac Disease Show Interindividual Fluctuating Myocardial TRPC6-Immunoreactivity

Elderly with Varying Extents of Cardiac Disease Show Interindividual Fluctuating Myocardial TRPC6-Immunoreactivity

TRPC6 promotes daunorubicin-induced mitochondrial fission and cell death in rat cardiomyocytes with the involvement of ERK1/2-DRP1 activation

TRPC6 is ubiquitously present in lymphatic tissues: A study using samples from body donors

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