1997
DOI: 10.1021/ac970183g
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Protein Digest Analysis by Pressurized Capillary Electrochromatography Using an Ion Trap Storage/Reflectron Time-of-Flight Mass Detector

Abstract: Pressurized capillary electrochromatography (pCEC) has been coupled to an ion trap storage/reflectron time-of-flight mass spectrometer for the analysis of peptide mixtures and protein digests. Taking advantage of the electroosmotic flow, high separation efficiency has been achieved in pCEC due to a relatively flat flow profile and the use of smaller packing materials. A supplementary pressure was used in these experiments which suppressed bubble formation and also allowed the tuning of the elution of peptides … Show more

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Cited by 147 publications
(97 citation statements)
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“…In this experiment, benzyl the sample solution was applied. It may be that the alcohol was selected as t marker, and k* can also be 0 excess of ions in the sample solution led to less of expressed theoretically as follows [12,34] the analytes being introduced. A similar result was m also found in CEC.…”
Section: Selectivity and Efficiencymentioning
confidence: 99%
“…In this experiment, benzyl the sample solution was applied. It may be that the alcohol was selected as t marker, and k* can also be 0 excess of ions in the sample solution led to less of expressed theoretically as follows [12,34] the analytes being introduced. A similar result was m also found in CEC.…”
Section: Selectivity and Efficiencymentioning
confidence: 99%
“…As we know, the retention factor in HPLC can be easily calculated from the migration time of the solute and the void time, which can be directly obtained from a chromatogram. Similarly, the k* in CEC was defined with the following equation by some authors [24,25]: electrochromatographic retention factor k* has been used to describe the migration process of charged solutes both in RP-CEC [24] and IE-CEC [12]. from that in CZE; such a result was also found in the Theoretically, k* can also be expressed as follows separation of small peptides by CZE and strong [24, The chromatographic retention factor k9 is always a purely chromatographic process.…”
Section: Introductionmentioning
confidence: 99%
“…The retention factor in HPLC can be easily calculated according to the migration time of an analyte and the void time, which is readily available from a chromatogram. Similarly, this definition of retention factor (k*) was applied to CEC according to some authors [14][15][16]:…”
Section: Basic Concepts Of Cecmentioning
confidence: 99%
“…The electrochromatographic retention factor (k*) has been used to describe the migration processes of charged solutes in both reversed-phase CEC and ion-exchange CEC. Following this definition, the electrochromatographic retention factor for a charged analyte can be given by the equation according to Wu et al [14]:…”
Section: Basic Concepts Of Cecmentioning
confidence: 99%