Protein Disulfide Isomerase-Like Protein 1-1 Controls Endosperm Development through Regulation of the Amount and Composition of Seed Proteins in Rice

Kim, Yeon Jeong; Yeu, Song Yion; Park, Bong Soo; Koh, Hee Jong; Song, Jong Tae; Seo, Hak Soo

doi:10.1371/journal.pone.0044493

Cited by 51 publications

(53 citation statements)

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“…It has been reported that prolyl hydroxylase has chaperone activity and inhibits the aggregation of misfolded proteins that play vital roles in the maturation of secreted plasma membrane and storage proteins (Kim et al, 2012). Prolyl hydroxylase directly provide disulfides to substrate proteins via thiol-disulfide exchange reactions.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

et al. 2016

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Vitamin A deficiency (VAD) is the leading cause of blindness among children and is associated with high risk of maternal mortality. In order to enhance the bioavailability of vitamin A, high carotenoid transgenic golden rice has been developed by manipulating enzymes, such as phytoene synthase (psy) and phytoene desaturase (crtI). In this study, proteome and metabolite analyses were carried out to comprehend metabolic regulation and adaptation of transgenic golden rice after the manipulation of endosperm specific carotenoid pathways. The main alteration was observed in carbohydrate metabolism pathways of the transgenic seeds. The 2D based proteomic studies demonstrated that carbohydrate metabolism-related enzymes, such as pullulanase, UDP-glucose pyrophosphorylase, and glucose-1-phosphate adenylyltransferase, were primarily up-regulated in transgenic rice seeds. In addition, the enzyme PPDK was also elevated in transgenic seeds thus enhancing pyruvate biosynthesis, which is the precursor in the carotenoids biosynthetic pathway. GC-MS based metabolite profiling demonstrated an increase in the levels of glyceric acid, fructo-furanose, and galactose, while decrease in galactonic acid and gentiobiose in the transgenic rice compared to WT. It is noteworthy to mention that the carotenoid content, especially β-carotene level in transgenic rice (4.3 μg/g) was significantly enhanced. The present study highlights the metabolic adaptation process of a transgenic golden rice line (homozygous T4 progeny of SKBR-244) after enhancing carotenoid biosynthesis. The presented information would be helpful in the development of crops enriched in carotenoids by expressing metabolic flux of pyruvate biosynthesis.

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Section: Discussionmentioning

confidence: 99%

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

et al. 2016

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“…Finally, materials were sectioned into 8 μm thick sections, which were stained with toluidine blue and were observed using a light microscope. Sections were viewed with Olympus BX53 microscope (Olympus, Japan), and were imaged using SPOT FLEX™ camera Whole or sectioned seeds were used for staining with methylene blue [28]. Ten grain seeds were used to measure the aleurone layers thickness.…”

Section: Histological and Cytological Analysismentioning

confidence: 99%

A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice

Wang

Zhou

et al. 2015

Plant Science

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“…Previous studies indicate that PDI mutations can result in irregular starch granule formation and chalky grain phenotypes (206). The rice mutant esp2 lacks protein disulfide isomerase-like (PDIL)1;1, but shows enhanced expression of the thiol disulphide oxidoreductase OsEro1 (207).…”

Section: Protein Disulphide Isomerases (Pdi)mentioning

confidence: 99%

“…The role of phosphorylation-specific isomerases in protein folding has reported effects on the structure and interactions of seed storage proteins (280,281). Proteins with phosphorylation activity identified with LC-MS/MS, included peptidyl-prolyl cis/trans isomerase (PPIase) and maize protein disulphide isomerase (PDI).…”

Section: Biological Significance Of Proteins Identified By Proteomic mentioning

confidence: 99%

“…PDI is involved in oxidative protein folding and regulates the accumulation of storage proteins in the endosperm. Mutations in PDI confer a chalky phenotype, with irregularly shaped protein bodies and starch granules (281). The reduced expression of PDI in rice is associated with the decreased activity of plastidial phosphorylase and pullalanase, as well as increased activity of soluble starch synthase 1 and ADP-glucose pyrophosphatase (267).…”

Section: Biological Significance Of Proteins Identified By Proteomic mentioning

confidence: 99%

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Grain Proteomics of Sorghum: Improving Digestibility, Nutritional Quality and Starch Conversion to Ethanol

Cremer¹

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Sorghum is an important food staple in rural Asia, India and Africa and a major feedstock in developed countries. Originating in the semi-arid African savannah, sorghum is well adapted to drought prone environments and produces more biomass per unit water than maize. Extensive variability exists within the sorghum gene pool creating a diversity of plant forms with many commercial and industrial applications. Sorghum, provides an alternative to grain crops with greater irrigation and fertiliser requirements, such as maize, however, the grain is generally less digestible, especially upon cooking, due to extensive disulphide cross-linking among sulphur-rich storage proteins in the protein-starch matrix. This reduces the accessibility of proteolytic enzymes to starch, decreasing palatability and nutritional value, and resulting in the need for increased processing.The commercial value of cereals is largely determined by the chemistry of the protein-starch matrix.Located on the periphery of storage protein bodies, cysteine-rich β-and γ-kafirins prevent enzymatic access to internally positioned α-kafirins and to starch. The development of mutants is an efficient approach for studying genetic regulation of protein biosynthesis and compartmentalisation and shows how changes in protein profile affect the physical characteristics of the seed. An integrated proteomic approach was employed to characterise 28 sorghum lines with allelic variation at the kafirin loci, including three β-kafirin null mutants, in order to determine the effects of kafirin genetic diversity on the expression of storage proteins.High performance liquid chromatography (HPLC) and capillary electrophoresis were employed to profile water/salt-and alcohol-soluble protein fractions, revealing a wide range of diversity in protein content across the genotypes. Peak profiles were similar among β-kafirin null lines but different to normal lines, with a significant reduction in alcohol-soluble protein and the disappearance of a major set of protein peaks. The relative content of sequentially extracted soluble, insoluble and residual proteins was measured using RP-HPLC to evaluate the degree of crosslinking among storage proteins in the seed. High levels of insoluble and residual protein were associated with reduced digestibility.Gel-based separation of seed proteins and subsequent identification with tandem mass spectrometry identified a range of redox-active proteins affecting storage protein biochemistry. Multiple protein fractions were analysed across the β-kafirin null line QL12 and wild-type line 296B. The redox states of endosperm proteins, of which kafirins are a subset, have been shown to impact on quality traits in addition to the expression of proteins. Thioredoxin, active in the processing of storage v proteins at germination, has reported impacts on grain digestibility and was differentially expressed across the genotypes. A range of putative uncharacterised sorghum proteins regulating the folding and disulphide pairing of storage proteins ...

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Protein Disulfide Isomerase-Like Protein 1-1 Controls Endosperm Development through Regulation of the Amount and Composition of Seed Proteins in Rice

Cited by 51 publications

References 57 publications

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

Metabolic Regulation of Carotenoid-Enriched Golden Rice Line

A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice

Grain Proteomics of Sorghum: Improving Digestibility, Nutritional Quality and Starch Conversion to Ethanol

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