2017
DOI: 10.1002/9783527699124.ch7
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Protein Engineering: Methods and Applications

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Cited by 2 publications
(2 citation statements)
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“…Directed evolution is a technique that does not require comprehensive structural information of the enzyme as it induces mutations to protein sequence randomly which mimics the Darwinian evolution. 42 One approach in directed evolution is the error-prone PCR, in which the components of the PCR mix are modified to induce errors in the protein-encoded gene sequence. 43 Rational design, in contrast, requires comprehensive structure information of target enzyme in order to generate enzyme with new or improved functions.…”
Section: Versatile Applications Of Subtilisinmentioning
confidence: 99%
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“…Directed evolution is a technique that does not require comprehensive structural information of the enzyme as it induces mutations to protein sequence randomly which mimics the Darwinian evolution. 42 One approach in directed evolution is the error-prone PCR, in which the components of the PCR mix are modified to induce errors in the protein-encoded gene sequence. 43 Rational design, in contrast, requires comprehensive structure information of target enzyme in order to generate enzyme with new or improved functions.…”
Section: Versatile Applications Of Subtilisinmentioning
confidence: 99%
“…Protein engineering typically utilizes two strategies known as directed evolution and rational design. Directed evolution is a technique that does not require comprehensive structural information of the enzyme as it induces mutations to protein sequence randomly which mimics the Darwinian evolution 42 . One approach in directed evolution is the error‐prone PCR, in which the components of the PCR mix are modified to induce errors in the protein‐encoded gene sequence 43 .…”
Section: Versatile Applications Of Subtilisinmentioning
confidence: 99%