Protein enzymatic hydrolysis integrated with ultrafiltration: Thermolysin application in obtaining peptides

Trusek-Hołownia, A.; Lech, Magdalena; Noworyta, A.

doi:10.1016/j.cej.2016.05.087

Cited by 10 publications

(2 citation statements)

References 25 publications

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“…Once the native protein is broken, fragments of the native protein (oligomers) form, which can be a substrate for the subsequent hydrolysis process, so it is a multisubstrate reaction [44], especially in mediums where no pure protein is available, but mixtures of innumerable proteins, such as in fish residues and in general in other agroindustrial waste. Due to the hydrolysis process, the molecular characteristics of the proteins change, because the average molecular weight of the protein fragments present decreases, this increases the surface load, causes the release of hydrophobic groups, and changes functional properties, among other effects [45].…”

Section: Enzymatic Hydrolysis Of Fish By-product Proteins 41 Protein ...mentioning

confidence: 99%

The Hydrolysates from Fish By-Product, An Opportunity Increasing

Montoya¹,

Sanchez²

2022

Biochemistry

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The fishery industries have continuously increased over the last decade. This growth comes accompanied by a high volume of by-products released to environment, because these industries discard between 60 and 70% of their production as waste. This waste includes fish whole or part from these such as fillet remains (15–20%), skin and fins (1–3%), bones (9–15%), heads (9–12%), viscera (12–18%) and scales (5%). This by-products are rich in proteins and lipids which of several nature, which can be recovered to obtain compounds of high added value. In this chapter, some methods to recover compounds from fish by-products will be discussed. Among others, will be discussed topics about postharvest of fish, by-product releasing, enzymatic hydrolysis of by-product and bioactive peptide obtaining from fish waste.

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Section: Enzymatic Hydrolysis Of Fish By-product Proteins 41 Protein ...mentioning

confidence: 99%

The Hydrolysates from Fish By-Product, An Opportunity Increasing

Montoya¹,

Sanchez²

2022

Biochemistry

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“…[20][21][22]. The enzymatic hydrolysis method is mild and easy to control, and most of the hydrolytic products are short peptides and amino acids, which conform to the requirements of food hygiene [23,24]. Therefore, bioactive peptide prepared by enzymatic hydrolysis is an effective way to transform proteins for its diversification for function foods.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Evaluation of Antioxidant Activities of Bioactive Peptides Obtained from Cornus officinalis

Zhang

Jiao

et al. 2022

Molecules

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The present study is a preparation of bioactive peptides from Cornus officinalis proteins by the compound enzymatic hydrolysis method. Response surface methodology (RSM) coupled with Box–Behnken design (BBD) is used to optimize the preparation process of Cornus officinalis peptides. The effects of independent variables, such as the amount of enzyme, pH value, time, extraction times and the ratio of material to liquid on the yield of peptides, are also investigated. The analysis results of the RSM model show that the optimum conditions for the extraction of Cornus officinalis peptides were a pH value of 6.76, temperature of 48.84 °C and the amount of enzyme of 0.19%. Under optimal conditions, the yield of peptides was 36.18 ± 0.26 %, which was close to the predicted yield by the RSM model. Additionally, the prepared Cornus officinalis peptides showed significant antioxidant activity; the scavenging rates of the peptides for DPPH and ·OH were 48.47% and 29.41%, respectively. The results of the cell proliferation assay revealed that the prepared Cornus officinalis peptides could promote embryo fibroblast cells proliferation and repair oxidative damage cells. These results have a practical application value in the design of novel functional food formulations by using Cornus officinalis.

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Analysis of the effect of pulsed light on the protein of Lactobacillus plantarum based on liquid mass spectrometry

Zhang

2023

Food Sci Biotechnol

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Protein enzymatic hydrolysis integrated with ultrafiltration: Thermolysin application in obtaining peptides

Cited by 10 publications

References 25 publications

The Hydrolysates from Fish By-Product, An Opportunity Increasing

The Hydrolysates from Fish By-Product, An Opportunity Increasing

Preparation and Evaluation of Antioxidant Activities of Bioactive Peptides Obtained from Cornus officinalis

Analysis of the effect of pulsed light on the protein of Lactobacillus plantarum based on liquid mass spectrometry

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