Protein expression profile characteristic to hepatocellular carcinoma revealed by 2D-DIGE with supervised learning

Teramoto, Reiji; Minagawa, Hiroko; Honda, Masao; Miyazaki, Kenji; Tabuse, Yo; Kamijo, Keita; Ueda, Teruyuki; Kaneko, Shuichi

doi:10.1016/j.bbapap.2008.02.011

Cited by 44 publications

(43 citation statements)

References 43 publications

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“…Overexpression of PDIA3 has been reported in hepatocellular carcinoma (HCC). 73 In our study, PDIA3 was 2.2-fold upregulated in the ESCC derived secretome. In IHC labeling for PDIA3, overexpression of PDIA3 was observed in 127/137 (93%) ESCC cases and the expression in majority of the cases was cytoplasmic and membranous.…”

Section: Validation Of Known Biomarker: Transforming Growth Factor Besupporting

confidence: 48%

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Kashyap

Harsha

Renuse

et al. 2010

Cancer Biology & Therapy

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Section: Validation Of Known Biomarker: Transforming Growth Factor Besupporting

confidence: 48%

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Kashyap

Harsha

Renuse

et al. 2010

Cancer Biology & Therapy

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“…HSP 70 isoforms, a known carcinogenic-promoting agent [57], was detected as upregulated protein in our present study. HSP 70 enhances the ability of tumor cells to alter biological functions of the cells [58].…”

Section: N C N C N C N Csupporting

confidence: 67%

Analysis of Differentially Expressed Proteins in Colorectal Cancer Using Hydroxyapatite Column and SDS-PAGE

Lim

Gooi²,

Singh³

et al. 2011

Appl Biochem Biotechnol

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Limitation on two dimensional (2D) gel electrophoresis technique causes some proteins to be under presented, especially the extreme acidic, basic, or membrane proteins. To overcome the limitation of 2D electrophoresis, an analysis method was developed for identification of differentially expressed proteins in normal and cancerous colonic tissues using self-pack hydroxyapatite (HA) column. Normal and cancerous colon tissues were homogenized and proteins were extracted using sodium phosphate buffer at pH 6.8. Protein concentration was determined and the proteins were loaded unto the HA column. HA column reduced the complexity of proteins mixture by fractionating the proteins according to their ionic strength. Further protein separation was accomplished by a simple and cost effective sodium dodecyl sulfate-polyacrylamide gel electrophoresis method. The protein bands were subjected to in-gel digestion and protein analysis was performed using electrospray ionization (ESI) ion trap mass spectrometer. There were 17 upregulated proteins and seven downregulated proteins detected with significant differential expression. Some of these proteins were low abundant proteins or proteins with extreme pH that were usually under presented in 2D gel analysis. We have identified brain mitochondrial carrier protein 1, T-cell surface glycoprotein CD1a, SOSS complex subunit B2, and Protein Jade 1 which were previously not detected in 2D gel analysis method.

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“…Condensin also contributes to mitosis-specific chromosome compaction and is required for proper chromosome segregation, although the functional significance of HCAP-G in the condensing complex is largely unknown (33,34). AKR1B10 (ARL1, aldose reductase-like 1) was originally isolated as a new member of the aldo-keto reductase superfamily overexpressed in HCC and is reportedly related to the histologic differentiation of HCC (35,36). AKR1B10 was also overexpressed in squamous cell carcinoma of the lung and its precursor conditions (37).…”

Section: Discussionmentioning

confidence: 99%

Combined Functional Genome Survey of Therapeutic Targets for Hepatocellular Carcinoma

Satow¹,

Shitashige²,

Kanai³

et al. 2010

Clinical Cancer Research

148

121

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Purpose: The outcome of patients with advanced hepatocellular carcinoma (HCC) has remained unsatisfactory. Patients with HCC suffer from chronic hepatitis or liver cirrhosis, and their reserve liver function is often limited.Experimental Design: To develop new therapeutic agents that act specifically on HCC but interfere only minimally with residual liver function, we searched for genes that were upregulated in 20 cases of HCC [namely, discovery sets 1 (n = 10) and 2 (n = 10)] in comparison with corresponding nontumorous liver and a panel representing normal organs using high-density microarrays capable of detecting all exons in the human genome.Results: Eleven transcripts whose expression was significantly increased in HCC were subjected to siRNAbased secondary screening of genes required for HCC cell proliferation as well as quantitative reverse transcription-PCR analysis [validation sets 1 (n = 20) and 2 (n = 44)] and immunohistochemistry (n = 19). We finally extracted four genes, AKR1B10, HCAP-G, RRM2, and TPX2, as candidate therapeutic targets for HCC. siRNA-mediated knockdown of these candidate genes inhibited the proliferation of HCC cells and the growth of HCC xenografts transplanted into immunodeficient mice.Conclusions: The four genes we identified were highly expressed in HCC, and HCC cells are highly dependent on these genes for proliferation. Although many important genes must have been overlooked, the selected genes were biologically relevant. The combination of genome-wide expression and functional screening described here is a rapid and comprehensive approach that could be applied in the identification of therapeutic targets in any type of human malignancy. Clin Cancer Res; 16(9); 2518-28. ©2010 AACR.

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Protein expression profile characteristic to hepatocellular carcinoma revealed by 2D-DIGE with supervised learning

Cited by 44 publications

References 43 publications

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome

Analysis of Differentially Expressed Proteins in Colorectal Cancer Using Hydroxyapatite Column and SDS-PAGE

Combined Functional Genome Survey of Therapeutic Targets for Hepatocellular Carcinoma

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