Protein Extraction and Stripping with Calixarene Derivatives

Abstract

Solvent extraction of cationic

Liquid membrane transport of cytochrome c using a calix[6]arene carboxylic acid derivative as a carrier

“…Previously, the extraction of Cyt-c using a cyclic tetramer t Oct[4]CH 2 COOH, hexamer t Oct[6]CH 2 COOH and octamer t Oct[8]CH 2 COOH was compared in order to elucidate the structural factors for a successful extraction [14,15]. The calix [6]arene resulted in the highest yield of Cyt-c in all extractants, calix [8] is the same as that for amino acids, as shown in our previous study [16][17][18].…”

“…1) was found to form a complex with a cationic protein cytochrome c (Cyt-c) [14,15]. The calix [6]arene has an ideal cavity size and C 3 symmetry for entrapment of a protonated amino group (-NH 3 + ) and has carboxylic acid groups that interact with -NH 3 + [16][17][18][19], which results in a strong affinity for the -amino group of lysine residues on the surface of lysine rich proteins such as Cyt-c.…”

Recognition of Lysine Residues on Protein Surfaces Using Calixarenes and its Application

“…In this article, the recognition of lysine residues on the surface of a protein with calixarene molecules and its application are summarized [18][19][20][21][22][23]. The selective extraction system according to the distribution of residues on the surface of the protein offers a new separation method for proteins.…”

Recognition of exterior protein surfaces using artificial ligands based on calixarenes, crown ethers, and tetraphenylporphyrins

“…Cytochrome c extracted using calix [6]arene can be quantitatively recovered into an aqueous solution under optimal conditions [76,77]. Because the extraction is based on the proton-exchange reaction, the extracted protein is stripped using an aqueous acidic solution.…”