Protein extraction from lupin seeds: microstructural aspects and hypothesis of mechanism

Abstract

Protein was extracted into water (pH 8.0,l h) from defatted lupin seed kernel particles (mean 8,1425 pm). Intact and extracted particles were examined by scanning and transmission electron microscopy. The inner surface of the cell wall initially showed several small holes considered as possible sites of plasmodesmata. During extraction, protein bodies first became disrupted into smaller microstructures and then protein dissolved. Wide passages were formed between cells which allowed transport of protein. The molecular weight of proteins in solution did not change with time, suggesting that bulk flow rather than selective transport predominated.

Aqueous Extraction of Oil and Protein From Soybean and Lupin: A Comparative Study

“…They reported simultaneous extraction of up to 70 and 31% of protein and oil, respectively. Similar to what was observed with soybean, particle size, temperature and pH impacted extraction yields of lupin compounds (Aguilera 1989;Aguilera and Garcia 1989). Use of flour, i.e., material with small particle size, usually increased aqueous extraction yields, but have the disadvantages of using an energy consuming size reduction step, and in the case of AEP of soy flour, entrapped the released oil in an emulsion that was rather stable toward demulsification treatments (Chabrand et al 2008).…”