Protein farnesylation negatively regulates brassinosteroid signaling via reducing BES1 stability in <i>Arabidopsis thaliana</i>

Feng, Zengxiu; Shi, Hongyong; Lv, Mei; Ma, Yuang; Li, Jia

doi:10.1111/jipb.13093

Cited by 6 publications

(4 citation statements)

References 61 publications

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“…In Arabidopsis , disruption of the prenylation of GTPases ROP2 reduced protein stability, which was dependent on the ubiquitin–26S proteasome system (Chai et al, 2016). Protein farnesylation promoted BES1 ubiquitination and negatively regulated brassinosteroid signalling (Feng et al, 2021). The effector AVR3a P. infestans modified the E3 ligase activity of CMPG1 to suppress CMPG1‐mediated cell death (Bos et al, 2010; Gilroy et al, 2011); E3 ligase activity was required for its regulatory role in promoting PCD triggered by the perception of pathogen molecules on the PM.…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis , the enhanced response to ABA 1 ( ERA1 ) encodes a β subunit of FTase. The era1 knockout mutant is more sensitive to ABA and BR (Cutler et al, 1996; Feng et al, 2021; Pei et al, 1998), and exhibits increased susceptibility to virulent bacterial and oomycete pathogens (Goritschnig et al, 2008). Isoprenylated avrRpt2‐induced gene 1 (AIG1) participates in RPS2‐mediated plant defence in response to bacterial strains carrying avrRpt2 (Reuber et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Isoprenylation modification is required for HIPP1‐mediated powdery mildew resistance in wheat

Wang

Zhang

et al. 2022

Plant Cell & Environment

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Powdery mildew (Pm), caused by Blumeria graminis f.sp. tritici (Bgt), is one of the most important wheat diseases. Heavy‐metal‐associated isoprenylated plant protein (HIPP1) has been proved playing important roles in response to biotic and a biotic stress. In present study, we proved HIPP1‐V from Haynalidia villosa is a positive regulator in Pm resistance. HIPP1‐V was rapidly induced by Bgt. Transiently or stably heterologous overexpressing HIPP1‐V in wheat suppressed the haustorium formation and enhanced Pm resistance. HIPP1‐V isoprenylation was critical for plasma membrane (PM) localization, interaction with E3‐ligase CMPG1‐V and function in Pm resistance. Bgt infection recruited the isoprenylated HIPP1‐V and CMPG1s on PM; blocking the HIPP1 isoprenylation reduced such recruitment and compromised the resistance of OE‐CMPG1‐V and OE‐HIPP1‐V. Overexpressing HIPP1‐VC148G could not enhance Pm resistance. These indicated the Pm resistance was dependent on HIPP1‐V's isoprenylation. DGEs related to the ROS and SA pathways were remarkably enriched in OE‐HIPP1‐V, revealing their involvement in Pm resistance. Our results provide evidence on the important role of protein isoprenylation in plant defense.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isoprenylation modification is required for HIPP1‐mediated powdery mildew resistance in wheat

Wang

Zhang

et al. 2022

Plant Cell & Environment

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“…The steroid phytohormone brassinosteroid (BR) plays an essential role in a broad range of plant growth and vital physiological processes ( Li and Deng, 2005 ; Wang et al., 2012 ; Anwar et al., 2018 ; Feng et al., 2021 ; Li et al., 2021 ). BR-INSENSITIVE1 (BRI1)-EMS SUPPRESSOR1 (BES1) and BRASSINAZOLE RESISTANT1 (BZR1) are core transcription factors directly mediating BR signaling ( Wang et al., 2012 ; Chen et al., 2019b ; Cui et al., 2019 ; Cao et al., 2020 ; Kono and Yin, 2020 ; Su et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…BES1 and BZR1 possess a conserved BES1-type domain in their N termini and belong to the plant-specific BES1/BZR1 transcription factor family, which consists of eight members in Arabidopsis ( Arabidopsis thaliana L.), 11 in maize ( Zea mays L.), 16 in soybean ( Glycine max L.), 22 in apple ( Malus domestica L.), and nine in tomato ( Solanum lycopersicum L.) ( Wang et al., 2012 ; Lachowiec et al., 2018 ; Li et al., 2018 ; Li et al., 2019b ; Li Y. et al, 2018 ; Cao et al., 2020 ; Feng et al., 2021 ; Su et al., 2021 ; Sun et al., 2022 ). The functions of the eight BES1/BZR1 family members in the model species Arabidopsis (BES1, BZR1, BEH1, BEH2, BEH3, BEH4, BMY2, and BMY4) have been well studied ( Yin et al., 2005 ; Guo et al., 2009 ; Reinhold et al., 2011 ; Anwar et al., 2018 ; Lachowiec et al., 2018 ; Chen et al., 2019b ; Otani et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

BRI1 EMS SUPPRESSOR1 genes regulate abiotic stress and anther development in wheat (Triticum aestivum L.)

Wang,

Zuo,

Liu

et al. 2023

Front. Plant Sci.

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BRI1 EMS SUPPRESSOR1 (BES1) family members are crucial downstream regulators that positively mediate brassinosteroid signaling, playing vital roles in the regulation of plant stress responses and anther development in Arabidopsis. Importantly, the expression profiles of wheat (Triticum aestivum L.) BES1 genes have not been analyzed comprehensively and systematically in response to abiotic stress or during anther development. In this study, we identified 23 BES1-like genes in common wheat, which were unevenly distributed on 17 out of 21 wheat chromosomes. Phylogenetic analysis clustered the BES1 genes into four major clades; moreover, TaBES1-3A2, TaBES1-3B2 and TaBES1-3D2 belonged to the same clade as Arabidopsis BES1/BZR1 HOMOLOG3 (BEH3) and BEH4, which participate in anther development. The expression levels of 23 wheat BES1 genes were assessed using real-time quantitative PCR under various abiotic stress conditions (drought, salt, heat, and cold), and we found that most TaBES1-like genes were downregulated under abiotic stress, particularly during drought stress. We therefore used drought-tolerant and drought-sensitive wheat cultivars to explore TaBES1 expression patterns under drought stress. TaBES1-3B2 and TaBES1-3D2 expression was high in drought-tolerant cultivars but substantially repressed in drought-sensitive cultivars, while TaBES1-6D presented an opposite pattern. Among genes preferentially expressed in anthers, TaBES1-3B2 and TaBES1-3D2 expression was substantially downregulated in thermosensitive genic male-sterile wheat lines compared to common wheat cultivar under sterile conditions, while we detected no obvious differences under fertile conditions. This result suggests that TaBES1-3B2 and TaBES1-3D2 might not only play roles in regulating drought tolerance, but also participate in low temperature-induced male sterility.

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Physiological and Transcriptomic Analyses of the Effects of SlBRI1 Expression Levels on the Drought Tolerance of Tomato Seedlings

Nie

Yang

Xiao

et al. 2022

J Plant Growth Regul

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Background: Brassinosteroids (BRs) not only in uence plant growth and development but also regulate the various stress responses of plants. acts as a BR receptor, sensing the BRs and then activating BR signaling. In this study, how SlBRI1 regulate the drought resistance of tomato have further been researched at physiological and transcriptomic level.Results: We obtained SlBRI1-overexpressing and SlBRI1 weak mutant (abs) plants in the same background to research the underlying mechanism in drought resistance. In this study, physiological analyses revealed that abs plants had a higher net photosynthetic rate and less wilting than MM plants; abs plants also had lower H 2 O 2 and O 2 − accumulation through higher antioxidant enzyme activities under drought conditions. RNA-Seq analysis showed that 768 (53.9%) of 1425 drought-induced genes and 418 (49.8%) of 840 drought-repressed genes were regulated by abs in the same direction under normal conditions. Moreover, 158 drought-induced and 43 drought-repressed genes are further upregulated and downregulated in abs plants under drought conditions, respectively. An in-depth analysis of these DEGs revealed that abs regulated the expression of genes related to ABA metabolism and polyamine biosynthesis as well as oxidoreductase activity genes under normal and drought conditions.Furthermore, analysis of transcription factor expression suggested that abs affected drought tolerance mainly through the regulation of WRKY, ERF, bHLH and MYB transcription factors. However, the expression of most of these genes was the same or opposite in SlBRI1OE plants compared to the MM group. Conclusion:Our results establish that SlBRI1 expression level was negatively involved in drought responses of tomato. Furthermore, our study provides valuable information for future breeding to appropriately reduce the expression of SlBRI1 and improve drought resistance without affecting plant growth.

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Protein farnesylation negatively regulates brassinosteroid signaling via reducing BES1 stability in Arabidopsis thaliana

Cited by 6 publications

References 61 publications

Isoprenylation modification is required for HIPP1‐mediated powdery mildew resistance in wheat

Isoprenylation modification is required for HIPP1‐mediated powdery mildew resistance in wheat

BRI1 EMS SUPPRESSOR1 genes regulate abiotic stress and anther development in wheat (Triticum aestivum L.)

Physiological and Transcriptomic Analyses of the Effects of SlBRI1 Expression Levels on the Drought Tolerance of Tomato Seedlings

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