1998
DOI: 10.1074/jbc.273.32.20015
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Protein Footprinting Reveals Specific Binding Modes of a High Mobility Group Protein I to DNAs of Different Conformation

Abstract: The high mobility group proteins I and Y (HMGI/Y) are abundant components of chromatin. They are thought to derepress chromatin, affect the assembly and activity of the transcriptional machinery, and associate with constitutive heterochromatin during mitosis. HMGI/Y protein molecules contain three potential DNA-binding motifs (AT-hooks), but the extent of contacts between DNA and the entire protein has not been determined. We have used a protein-footprinting procedure to map regions of the Chironomus HMGI prot… Show more

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Cited by 29 publications
(40 citation statements)
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“…A similar situation was described previously for the Chironomus HMGI protein (16); however, the insect protein binds to the promoter of the ␤-interferon gene using the second and the third motif. Interestingly, in both cHMGI and HMGI-C, the distance between the centers of those AT-hooks that bind to the promoter is exactly 20 residues.…”
Section: Phosphorylation By Cdc2 Kinase Reduces the Extent Of Contactmentioning
confidence: 76%
See 2 more Smart Citations
“…A similar situation was described previously for the Chironomus HMGI protein (16); however, the insect protein binds to the promoter of the ␤-interferon gene using the second and the third motif. Interestingly, in both cHMGI and HMGI-C, the distance between the centers of those AT-hooks that bind to the promoter is exactly 20 residues.…”
Section: Phosphorylation By Cdc2 Kinase Reduces the Extent Of Contactmentioning
confidence: 76%
“…Binding of HMGI-C[P CK2 ] resulted in the protection of the IFN-␤ promoter at bases 17-23 and 25-32 of the top strand (Fig. 6A, black bars) and bases [13][14][15][16][17]19, and 22-31 of the bottom strand (Fig. 6B, black bars).…”
Section: Phosphorylation By Cdc2 Kinase Reduces the Extent Of Contactmentioning
confidence: 99%
See 1 more Smart Citation
“…3). Similarly, protein hydroxyl radical footprinting analysis of HMGA showed that DBD1, which is identical in sequence to TAF1 AT-hooks, but not surrounding residues contact INF-␤ DNA (35). In contrast, residues surrounding DBD2 and DBD3 substantially contribute to DNA binding.…”
Section: Discussionmentioning
confidence: 99%
“…1, 2A, and 5A) (31-33). INF-␤, which contains PRDIII-1, PRDII, and NRDI enhancer elements, was chosen as a probe because it is bound by HMGA AT-hooks in vivo and in vitro (26,(31)(32)(33)(34)(35)(36)(37). TAF1-2 and TAF1-4, the isoforms that contain two AT-hooks, altered the mobility of the INF-␤ probe, indicating that they bind INF-␤ DNA (Fig.…”
Section: Taf1 Alternative Exon 12a Encodes An At-hook Motif-mentioning
confidence: 99%