Protein-free and low-protein media for the cultivation of Leptospira

Bey, R.; Johnson, Russell C.

doi:10.1128/iai.19.2.562-569.1978

Cited by 49 publications

(15 citation statements)

References 19 publications

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“…Leptospira interrogans serovar pomona H10-414 was maintained at 30°C in bovine serum albumin-Tween 80 medium (8). Virulence was maintained by hamster back passage as previously described (7).…”

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confidence: 99%

“…Virulence was maintained by hamster back passage as previously described (7). One volume of inoculum culture was added to 200 volumes of protein-free culture medium prepared as previously described (8), allowed to stand at 30°C for 3 days, and then aerated on a gyratory shaker at 30°C for 3 to 4 days. Final cell densities of 8 x 108 to 9 x 108 leptospires per ml were obtained.…”

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confidence: 99%

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Copurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C

Bernheimer¹,

Bey²

1986

Infect Immun

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confidence: 99%

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confidence: 99%

Copurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C

Bernheimer¹,

Bey²

1986

Infect Immun

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“…In this study, leptospira was cultivated in PFM supplemented with 0.1% bovine serum albumin. It was reported that immunogenicity of leptospires cultivated in PFM was similar to those prepared in serum-containing media (8,9). Reduction of any serum or albumin concentration in medium is an effective means for avoiding the adverse reaction of vaccine in the immunized host.…”

Section: Discussionmentioning

confidence: 86%

Comparison of Protective Effects with Tetra‐Valent Glycolipid Antigens and Whole Cell‐Inactivated Vaccine in Experimental Infection of Leptospira

Masuzawa

Suzuki

Yanagihara

1991

Microbiology and Immunology

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The protective antigens (PAgs), glycolipid substance, were extracted from Leptospira interrogans serovars autumnalis, hebdomadis, australis and copenhageni, which were considered as main causal serovars of human leptospirosis in Japan, with chloroform‐methanol‐water (1:2:0.8, [vol/vol/vol]) solution. The tetra‐valent formalin‐inactivated leptospiral vaccine (Weil's disease and Akiyami combined vaccine) composed of the four serovars mentioned are used as vaccine to protect human from leptospiral infection in Japan. The protective effect, agglutinating antibody‐inducing activity and opsonin‐inducing activity of tetra‐valent PAgs were compared with those of vaccines now in use, which were supplied by two companies, Takeda Chemical Industries, Ltd., and Denka‐Seiken Co., in Japan. The tetra‐valent PAgs which contained 10 μg of each PAg protected hamsters and cyclophosphamide‐treated mice from lethal infection of serovar copenhageni and induced agglutinating antibodies against the four serovars in the same degrees as vaccines. These results suggested that the tetra‐valent PAgs might be useful as a component vaccine against leptospiral infection instead of formalized whole cells vaccines for human.

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“…TM antigen. Leptospira interrogans serovar canicola strain Hond Utrecht-IV was grown in Bey and Johnson's protein-free medium (5) at 30 C for 1 week. Serovar specific TM antigen was extracted and purified from the organisms as previously described (16).…”

Section: Methodsmentioning

confidence: 99%

Isolation of Antigenically Active Components from Leptospiral Serovar‐Specific Lipopoly‐saccharide Antigen by Alkaline Treatment

Tsuji

Kawaoka

Naiki

et al. 1981

Microbiology and Immunology

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The serovar-specific main antigen (TM antigen) of Leptospira interrogans serovar canicola, which has lipopolysaccharide properties, was treated with 0.1 N sodium hydroxide. This treatment degraded the antigen into two major antigenic components, one of high and one of low molecular weight. The component with the lower molecular weight (approximately 4,000 daltons) consisted mainly of carbohydrates, having lost almost all of the fatty acid and protein components of the original antigen. Although the substance lacked immunoprecipitable activity, it continued to show serovar-specific inhibitory potency in a radioimmunoassay system as well as in a microscopic immunoagglutination reaction of the organisms. The antigenic activity of the compound was also reduced by periodate oxidation as was that of the TM antigen. A component with the same chemical and physicochemical properties was also produced by alkaline treatment from a different serotype TM antigen (serovar kremastos Kyoto), but it showed no antigenic activity. Leptospiral serovar-specific main (TM) antigen has been extracted from several organisms of different serovars. The TM antigen possesses chemical and physicochemical properties similar to those of the lipopolysaccharide antigens of gram-negative bacteria (1,2, 10, 15, 16). The antigenic activity of TM antigen is destroyed by periodate oxidation (10, 11). A carbohydrate portion demonstrating antigenic activity was isolated from the TM antigen of serovar kremastos Kyoto by formic acid hydrolysis (Y. Kawaoka, M. Naiki, and R. Yanagawa, in preparation) .In this study, TM antigen was treated with mild alkali to cleave the ester bonds, and it was found that this treatment also produced a carbohydrate fragment with antigenic activity.

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Protein-free and low-protein media for the cultivation of Leptospira

Cited by 49 publications

References 19 publications

Copurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C

Copurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C

Comparison of Protective Effects with Tetra‐Valent Glycolipid Antigens and Whole Cell‐Inactivated Vaccine in Experimental Infection of Leptospira

Isolation of Antigenically Active Components from Leptospiral Serovar‐Specific Lipopoly‐saccharide Antigen by Alkaline Treatment

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