Protein Glutathiolation

Ghezzi, Pietro; Simplicio, P. Di

doi:10.1002/9783527627585.ch5

Cited by 9 publications

(12 citation statements)

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“…However, oxidative stress is the principal force in regulating redox signaling (Ghezzi and Di Simplicio 2009;Sohal and Orr 2012). Moreover, the glutathione role in cell signaling could be independent from oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…However, considering the role of glutathione in cell signaling (Jones 2006;Ghezzi and Di Simplicio 2009) and the fact that this antioxidant is present in every tissue (Wu et al 2004), a general regulatory mechanism can also be proposed. At first glance, the results suggest a compensatory (hormetic) response against a mild stressor, which may improve fitness (Costantini et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Studies in rodents have shown that restriction in the availability of certain energy sources in the diet (i.e., proteins) during development reduces oxidative stress and, ultimately, increases life span (e.g., Tarry-Adkins et al 2007;Chen et al 2009 and references therein). Additionally, oxidative stress influences redox signals, governing important physiological pathways and modifying gene expression (Jones 2006;Ghezzi and Di Simplicio 2009). A key molecule in this framework is glutathione.…”

Section: Introductionmentioning

confidence: 99%

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The Level of an Intracellular Antioxidant during Development Determines the Adult Phenotype in a Bird Species: A Potential Organizer Role for Glutathione

Romero-Haro

Alonso‐Álvarez

2015

The American Naturalist

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Online enhancement: appendix. Dryad data: http://dx.doi.org/10.5061/dryad.1rd58.abstract: Life-history traits are often involved in trade-offs whose outcome would depend on the availability of resources but also on the state of specific molecular signals. Early conditions can influence trade-offs and program the phenotype throughout the lifetime, with oxidative stress likely involved in many taxa. Here we address the potential regulatory role of a single intracellular antioxidant in lifehistory trade-offs. Blood glutathione levels were reduced in a large sample of birds (zebra finch Taeniopygia guttata) during development using the synthesis inhibitor buthionine sulfoximine (BSO). Results revealed several modifications in the adult phenotype. BSO-treated nestlings showed lower glutathione and plasma antioxidant levels. In adulthood, BSO birds endured greater oxidative damage in erythrocytes but stronger expression of a sexual signal. Moreover, adult BSO females also showed weaker resistance to oxidative stress but were heavier and showed better body condition. Results suggest that low glutathione values during growth favor the investment in traits that should improve fitness returns, probably in the form of early reproduction. Higher oxidative stress in adulthood may be endured if this cost is paid later in life. Either the presence of specific signaling mechanisms or the indirect effect of increased oxidative stress can explain our findings.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Level of an Intracellular Antioxidant during Development Determines the Adult Phenotype in a Bird Species: A Potential Organizer Role for Glutathione

Romero-Haro

Alonso‐Álvarez

2015

The American Naturalist

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“… 119 Once formed, disulfides are relatively stable to most physiological nucleophiles and are generally cleaved by other thiols as in thiol-disulfide exchange (nucleophilic substitution) reactions (Figure 5 ). 120 The thiol in a disulfide with the lower p K a will be the better leaving group and often dictates which cysteine is released in thiol-disulfide exchange. Indeed, this strategy is employed by the thiol-disulfide exchange catalysts in the cell, such as protein disulfide isomerases (PDI).…”

Section: Reactive Oxygen Species (Ros) In Biological Systemsmentioning

confidence: 99%

Cysteine-Mediated Redox Signaling: Chemistry, Biology, and Tools for Discovery

2013

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“…Under oxidative conditions, excessive GSSG can react with thiol groups of proteins, a process known as glutathionylation, leading to alterations of protein functioning (Hawkins et al, 2010;Hurd et al, 2005). The loss of cellular GSH/GSSG redox control makes glutathionylation a deleterious event (Ghezzi and Di Simplicio, 2009), and therefore GSSG is generally exported from the cell to the extracellular matrix (Garcia et al, 2010;Han et al, 2006). Given this, our findings suggest that juvenile C. gigas antioxidant capacity at the highest hypercapnia level (pH 7.0) was exceeded, with excess glutathione oxidation, and GSSG excretion resulting in lower total glutathione content, as seen in other bivalve species experiencing oxidative stress (Hannam et al, 2010;Peña-Llopis et al, 2002Regoli et al, 1998.…”

Section: Antioxidant Scavengers and Cellular Damagementioning

confidence: 99%

Native and exotic oysters in Brazil: Comparative tolerance to hypercapnia

Moreira

Figueira

Pecora

et al. 2018

Environmental Research

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Environmental hypercapnia in shallow coastal marine ecosystems can be exacerbated by increasing levels of atmospheric CO. In these ecosystems organisms are expected to become increasingly subjected to pCO levels several times higher than those inhabiting ocean waters (e.g.: 10,000µatm), but still our current understanding on different species capacity to respond to such levels of hypercapnia is limited. Oysters are among the most important foundation species inhabiting these coastal ecosystems, although natural oyster banks are increasingly threatened worldwide. In the present study we studied the effects of hypercapnia on two important oyster species, the pacific oyster C. gigas and the mangrove oyster C. brasiliana, to bring new insights on different species response mechanisms towards three hypercapnic levels (ca. 1,000; 4,000; 10,000 µatm), by study of a set of biomarkers related to metabolic potential (electron transport system - ETS), antioxidant capacity (SOD, CAT, GSH), cellular damage (LPO) and energetic fitness (GLY), in two life stages (juvenile and adult) after 28 days of exposure. Results showed marked differences between each species tolerance capacity to hypercapnia, with contrasting metabolic readjustment strategies (ETS), different antioxidant response capacities (SOD, CAT, GSH), which generally allowed to prevent increased cellular damage (LPO) and energetic impairment (GLY) in both species. Juveniles were more responsive to hypercapnia stress in both congeners, and are likely to be most sensitive to extreme hypercapnia in the environment. Juvenile C. gigas presented more pronounced biochemical alterations at intermediate hypercapnia (4,000µatm) than C. brasiliana. Adult C. gigas showed biochemical alterations mostly in response to high hypercapnia (10,000µatm), while adult C. brasiliana were less responsive to this environmental stressor, despite presenting decreased metabolic potential. Our data bring new insights on the biochemical performance of two important oyster species, and suggest that the duration of extreme hypercapnia events in the ecosystem may pose increased challenges for these organisms as their tolerance capacity may be time limited.

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Protein Glutathiolation

Cited by 9 publications

References 88 publications

The Level of an Intracellular Antioxidant during Development Determines the Adult Phenotype in a Bird Species: A Potential Organizer Role for Glutathione

The Level of an Intracellular Antioxidant during Development Determines the Adult Phenotype in a Bird Species: A Potential Organizer Role for Glutathione

Cysteine-Mediated Redox Signaling: Chemistry, Biology, and Tools for Discovery

Native and exotic oysters in Brazil: Comparative tolerance to hypercapnia

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