Protein heterogeneity in European wheat landraces and obsolete cultivars: Additional information

Gregová, E.; Hermuth, J.; Kraic, Ján; Dotlačil, L.

doi:10.1023/b:gres.0000024749.04012.ae

Cited by 20 publications

(25 citation statements)

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“…The allele Glu-B1an (subunit 6) was rare and was found only in two accessions (Odesskaya 16 and Saumur d'Automne). This subunit was previously detected in two landraces of bread wheat by Rodriquez-Quijano et al (1990) and Gregova´et al (1999). Relatively rare allele Glu-B1i (subunits 17 + 18), associated with good bread-making quality, was found in two accessions.…”

Section: Resultsmentioning

confidence: 51%

“…Hammer et al (1996) compared collection missions in Albania and Italy during different time periods and concluded that genetic erosion had occurred at similar rates in both places. Using electrophoretic analyses of wheat glutenins it is sometimes possible to detect new HMW-GS alleles also in landraces (van Hintum and Ellings 1991;Tahir et al 1996;Gregova´et al 1999;Juha´sz et al 2001;Gregova´et al 2004). The aim of this study was to continue in the analysis of HMW-GS pattern in European wheat landraces and obsolete cultivars started several years ago (Gregova´et al 1999;Gregova´et al 2004).…”

Section: Introductionmentioning

confidence: 95%

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Protein Heterogeneity in European Wheat Landraces and Obsolete Cultivars: Additional Information II

Gregová

Hermuth

Kraic

et al. 2006

Genet Resour Crop Evol

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The endosperm storage protein of 46 European wheat (Triticum aestivum L.) landraces and obsolete cultivars have been fractionated by SDS-PAGE to determine the composition of high molecular weight glutenin subunits (HMW-GS) composition. It has been discovered that about 46% of the wheats were heterogeneous, comprising 2-11 different glutenin profiles. Eighteen of them were observed to be homogeneous. A total of 13 HMW-GS alleles, including 3 at the Glu-A1, 8 at the Glu-B1, and 3 at the Glu-D1 loci were revealed. HMW-GS null controlled by locus Glu-A1, subunits 7 + 8 by Glu-B1, and 2 + 12 by Glu-D1 predominated. However low frequented alleles such as 17 + 18, 20, 6, and 7 were observed. Furthermore, other new alleles encoding HMW-GS at the locus Glu-B1 have been found in one of France cultivar (Saumur d'Automne). The glutenin-based quality score ranged from 4 to 10.

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Section: Resultsmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 95%

Protein Heterogeneity in European Wheat Landraces and Obsolete Cultivars: Additional Information II

Gregová

Hermuth

Kraic

et al. 2006

Genet Resour Crop Evol

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“…Because of its agricultural importance, the genetic properties of the HMW glutenin subunit have been extensively studied in various wheat landraces (Nakamura 2000, Zhang et al 2002, Popa et al 2003, Gregova et al 2006. Compared to previous studies, the characteristics of the HMW glutenin subunit of Afghan wheat were more similar to those of East Asian wheat rather than European wheat.…”

Section: The Hmw Glutenin Subunit In Afghanistanmentioning

confidence: 98%

“…To assess the diversity of agronomic traits and to discover new alleles that would improve bread-making quality, a number of studies have surveyed the genetic diversity of HMW glutenin subunit composition in wheat landraces collected from various countries, e.g. Japan (Nakamura 2000), China (Zhang et al 2002, Cong et al 2005, Pakistan (Tahir et al 1996), and European countries (Juhász et al 2003, Popa et al 2003, Gregova et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genetic diversity of an Afghan wheat collection based on morphological variation, HMW glutenin subunit polymorphisms, and AFLP

et al. 2009

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To reveal the genetic properties of an Afghan wheat collection maintained in Kyoto University, variations in morphological character and HMW glutenin subunit composition were investigated in 410 Afghan wheat accessions together with 65 accessions of Iranian and Pakistani wheat. The majority of the samples had primitive morphological characteristics, namely, tall plant height and lax spikes with a spelt head. The most frequent combination of HMW glutenin subunit was Glu-A1c (encoding subunit null), Glu-B1b (7 + 8), and Glu-D1a (2 + 12). Alleles preferable for modern breeding such as Glu-B1c and Glu-D1d were rare in our samples. Consequently, we concluded that the Afghan wheat accessions investigated in this study have genetic characteristics typical of landraces. As for their genetic diversity, the level of variation in the HMW glutenin subunit in Afghanistan was found to be equal to or lower than that in the neighboring countries. The largest variation was observed in Western Iran and Pakistan. AFLP analysis using 91 representative accessions also showed a comparatively low level of diversity in Afghanistan. These results suggest that a decrease in genetic diversity has occurred in Afghanistan. A novel HMW glutenin subunit (2.8) was found in this study, indicating the potential usefulness of Afghan landraces for wheat breeding.

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“…According to our previous observations, the frequency of the discovery of a novel HMW-GS in hexaploid wheat (Triticum aestivum L.) is very low, especially within the modern cultivars. Nevertheless, the screening of the collections of the maintained landraces and obsolete cultivars could increase the probability of finding novel or very lowly expressed HMW-GSs, even using such a simple analytical approach as SDS-PAGE (Gregová et al 2004) and landraces can contribute also breeding programes at present (Dotlačil et al 2010). The nucleotide sequence of the gene 1Dy12.3 and the deduced amino acid sequence of the protein were deposited in the GenBank database (GenBank: EF472958, Gregová & Mihálik 2007, unpublished).…”

Section: Glutenin Extraction and Hmw-gs Analysismentioning

confidence: 99%

Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.)

Mihálik¹,

Gregová²,

Galuszka³

et al. 2012

CZECH J GENET PLANT

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Abstract:A novel high-molecular-weight glutenin subunit encoded by the Glu-1D locus was identified in hexaploid wheat (Triticum aestivum L.) cultivar Noe and was designated as 1Dy12.3. This subunit differed in SDS-PAGE mobility from the well-known 1Dy10 and 1Dy12 subunits that are also encoded by this locus. An analysis of the gene sequences confirmed the uniqueness of 1Dy12.3 and revealed that it is most closely related to the 1Dy12 subunit. The size of the deduced protein was calculated to be 67 884 Da, which is different from the 1Dy10 and 1Dy12 subunits (67 475 Da and 68 713 Da, respectively). The 1Dy12.3 protein consists of 652 residues, with a highly conserved signal sequence and N-and C-terminal domains, although the central repetitive domain comprising motifs of hexapeptide (PGQGQQ) and nonapeptide (GYYPTSLQQ) repeats was less conserved. The 1Dy12.3 subunit demonstrates fewer QHPEQG hexapeptide motifs and exhibits an increased number of methionine residues in comparison to the other characterised high-molecular-weight glutenin subunits. The 1Dy12.3 subunit was cloned and expressed in Escherichia coli and was detected with a prolamin-specific antibody. The size of the detected immunocomplex corresponded to the native 1Dy12.3 protein isolated from grains. The existence and characterisation of this novel high-molecular-weight glutenin subunit increases the diversity of the glutenins encoded by the Glu-1D locus.

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Protein heterogeneity in European wheat landraces and obsolete cultivars: Additional information

Cited by 20 publications

References 0 publications

Protein Heterogeneity in European Wheat Landraces and Obsolete Cultivars: Additional Information II

Protein Heterogeneity in European Wheat Landraces and Obsolete Cultivars: Additional Information II

Evaluation of the genetic diversity of an Afghan wheat collection based on morphological variation, HMW glutenin subunit polymorphisms, and AFLP

Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.)

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