Protein hydrolysate waste of whitemouth croaker (Micropogonias furnieri) as a way of adding value to fish and reducing the environmental liabilities of the fishing industry

Abstract

ABSTRACT.As an alternative use of natural marine resources, processing waste from whitemouth croaker was used as raw material for the production of fish protein hydrolysate (FPH). The biometric characterization of the raw material was determined, and the optimal conditions of the protease established using DH (degree of hydrolysis) as the criterion. Bromatological analyses were performed according to AOAC methods. The DH were determined by the TCA (trichloroacetic acid) method and expressed as a percentage of solubilized protein after hydrolysis compared to the total protein in raw material. The average yields of viscera, heads and carcasses were 7.4, 36.3 and 56.3%, respectively. The optimal conditions for enzyme action on the viscera were: 55°C, hydrolysis time of 90 min, and enzyme concentration of 0.4 mg mL . The dry matter of hydrolysate from viscera yielded 73.5% supernatant (soluble fraction) and 27.5% precipitate (insoluble fraction), while that from muscle yielded 45.7% and 54.3%, respectively. The hydrolysate from viscera presented 86.0% proteins, 0.4% lipids and 4.4% ashes in the soluble fraction, and 51.0% proteins, 25.8% lipids and 5.1% ashes in the insoluble fraction. The hydrolysate from muscle presented 86.0% proteins, 0.4% lipids and 9.0% ashes in the soluble fraction, and 87.0% proteins, 4.4% lipids and 2.4% ashes in the insoluble fraction. A lower degree of hydrolysis of hydrolysate from muscle than that produced from viscera was observed, demonstrating the different interactions between the enzyme and the residues studied. Keywords: Micropogonias furnieri, fish protein hydrolysate, fish waste, protease.Hidrolizado proteico de desechos de corvina (Micropogonias furnieri) como una manera de agregar valor a los peces y reducir los pasivos ambientales de la industria pesquera RESUMEN. Como una alternativa de uso de los recursos marinos naturales, se emplean como materia prima los residuos del procesamiento de la corvina, para la producción de hidrolizado de proteínas de pescado (FPH). Se determinó la caracterización biométrica de la materia prima y se establecieron las condiciones óptimas de la proteasa, usando el GH (grado de hidrólisis) como criterio. Los análisis bromatológicos se realizaron de acuerdo con los métodos de AOAC. El GH se determinó por el método de TCA (ácido tricloroacético) y se expresó en porcentaje (%) de proteína solubilizada después de la hidrólisis, en comparación con la proteína total de la materia prima. Los rendimientos medios de vísceras, cabezas y cuerpos fueron de 7,4; 36,3 y 56,3% respectivamente. Las condiciones óptimas para la acción enzimática sobre las vísceras fueron: 55°C, tiempo de hidrólisis de 90 min y concentración enzimática de 0,4 mg mL -1 . Para el músculo, las condiciones establecidas fueron 45°C, 90 min y 0,8 mg mL respectivamente. El hidrolizado de vísceras presentó 86,0% de proteínas, 0,4% de lípidos y 4,4% de cenizas en la fracción soluble, y 51,0% de proteínas, 25,8% de lípidos y 5,1% de cenizas en la fracción insoluble. El hidrolizado a partir de...

Influence of enzymatic hydrolysis conditions on biochemical and antioxidant properties of pacific thread herring (Ophistonema libertate) hydrolysates

“…This pattern may be related to the fact that HPHs produced with 1% w/v enzyme were not extensively hydrolyzed (reaching a maximum of 12.7% DH), so that part of the protein content in herring muscle was not solubilized, being subsequently removed in the precipitated fraction during the centrifugation process. Other authors have reported ranges of percent protein content (78-80%) in fish muscle hydrolysates that are consistent with the results obtained in this study (Oliveira de Amorim et al, 2016;Ben Slama-Ben Salem et al 2017). Considering the high protein content in HPHs, these show potential for use as a nutritional supplement in the preparation of animal feed, mainly for carnivorous species with a high protein demand.…”

Production and characteristics of fish protein hydrolysate from parrotfish (Chlorurus sordidus) head

“…The fat content of parrotfish head FPH was lower than the FPH from croaker fish head waste (5.1 ± 4.0%) (Amorim et al, 2016) and commercial FPH (19–22%) (International Quality Ingredients (IQI), 2005). The low-fat content of parrotfish head FPH was due to the low-fat content in fish head raw materials (3.92%).…”

Peer Review #3 of "Production and characteristics of fish protein hydrolysate from parrotfish (Chlorurus sordidus) head (v0.1)"

“…Based on its protein content, FPH from parrotfish heads could be classified as a type B hydrolysate. The fat content of parrotfish head FPH was lower than the FPH from croaker fish head waste (5.1±4.0%) (Amorim et al, 2016) and commercial FPH (19-22%) (IQI, 2005). The low-fat content of parrotfish head FPH was due to the low-fat content in fish head raw materials (3.92%).…”