Protein immobilization to polystyrene via long poly(ethylene lycol) chains

Bergström, Karin; Holmberg, Krister

doi:10.1002/bit.260380817

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…Adsorption from solution may also be used for modifying solid surfaces, e.g., to give the surfaces protein-repellent properties. 1 In such cases it is preferable to use macromolecules, particularly charged ones. The reason is that the macromolecules can anchor to the surface by several segments and introduction of charges on the polymer with opposite sign to that of the surface tends to increase its affinity to the surface.…”

Section: Introductionmentioning

confidence: 99%

“…Of these methods, adsorption from solution is by far the simplest and is most commonly used when stabilizing or destabilizing colloidal particles. Adsorption from solution may also be used for modifying solid surfaces, e.g., to give the surfaces protein-repellent properties . In such cases it is preferable to use macromolecules, particularly charged ones.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Polyelectrolyte Charge Density on the Adsorption and Desorption Behavior on Mica

et al. 2002

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The XPS (ESCA) method was employed to quantitatively determine polyelectrolyte adsorption on the mica basal plane from low ionic strength solutions. Particular emphasis was given to the effect of the polyelectrolyte charge density. By combining the results obtained from XPS and surface force measurements it was possible to analyze the cation exchange at the surface that occurs as a result of polyelectrolyte adsorption. AFM-imaging was used to obtain information on the structure of the adsorbed layer when the polyelectrolyte coverage was low. Further, the desorption of preadsorbed polyelectrolyte layers by addition of inorganic salt and by addition of an anionic surfactant was investigated by XPS and some complementary surface force measurements. The results demonstrate that the lower the polyelectrolyte charge density is, the easier it is to remove the polyelectrolyte from the surface. The surfactant, which by itself does not adsorb to the mica surface, is more efficient in this respect than the inorganic salt. This observation can be rationalized by considering that the surfactant and polyelectrolyte form complexes with each other. Thus, the surfactant brings negative charges into the adsorbed layer that reduces the affinity to the surface. However, high-charge-density polyelectrolytes are removed to a very limited degree even when the surfactant concentration is above the critical micellar concentration, which is explainable by the poor solubility of the polyelectrolyte−surfactant complexes formed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Polyelectrolyte Charge Density on the Adsorption and Desorption Behavior on Mica

et al. 2002

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“…5,13,17,35,36 Glycidyl ether (epoxide)-functionalized PEGs (E-PEGs) have been used to coat surfaces and tether enzymes to glass particles. [35][36][37][38] The present work involves use of microparticle capillary electrophoresis, paired with electrokinetic modeling 18,27,33 to examine the utility of E-PEG for covalently coating aminosilane-modified quartz (Figure 1). Electrophoresis provided information related to quartz surface chemistry and polymer grafting density.…”

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confidence: 99%

Grafting Poly(ethylene glycol) Epoxide to Amino-Derivatized Quartz: Effect of Temperature and pH on Grafting Density

1996

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Microparticle capillary electrophoresis was used to characterize the surface of quartz capillaries grafted with the glycidyl ether of poly(ethylene glycol) (E-PEG). Site dissociation modeling of capillary electrokinetic behavior provided estimates of surface group pK and density, plus the distance (d) from the surface to the hydrodynamic plane of shear. Native quartz appeared to possess silanol groups of pK 3.6 and 6.9 whose surface densities varied with quartz treatment. Aminopropylsilane derivatization of quartz silanol groups in toluene yielded a coating which was stable (>6 h) at pH 10.3 and 60 °C. Aqueous grafting of E-PEG to this surface was relatively independent of pH (7.3-10.3) and reaction time (6-24 h) but was significantly influenced by reaction temperature (25-95 °C) and salt composition. PEG-grafted capillaries exhibited greatly reduced electroosmosis from pH 2 to 11. Significant grafting could be obtained under mild conditions (6 h, 35 °C, 0.4 M K(2)SO(4), pH 6.9). These results suggest that PEG chains increasingly extend normal to a surface as their grafting density increases, and that PEG conformation influences grafting density. The methods described should aid the use of PEG-coated surfaces in a variety of applications.

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“…The laying of the albumin onto the PEG brushes makes the interactions between the albumin and the rigid support softer, thus preserving the albumin’s natural conformation. Furthermore, the presence of PEG on the surface prevents surface bio-fouling [ 17 ]. Albumin has also been attached to polypropylene membranes coated with polyethylene glycol methacrylate brushes, where the polyethylene glycol methacrylate improves the material’s biocompatibility [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Poly(styrene-co-methyl methacrylate) Particles for Selective Removal of Bilirubin

et al. 2022

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Hyperbilirubinemia is one of the main causes of death in patients with severe hepatic problems, which justifies the research for bilirubin removal solutions. In this study, St-MMA particles with PEGMA and/or GMA brushes were synthesized. First, the recipe for St-MMA was optimized and then adapted for PEGMA and GMA incorporation. Different solvents were then assayed to improve the BSA immobilization capacity of the particles. Ethyl lactate proved to be the best solvent, reaching a BSA immobilization capacity improvement of up to 60% for St-MMA-GMA-PEGMA particles. These particles also presented the best results for BR removal from PBS. No significant differences in the final capacity for BR removal from PBS media were observed when BSA was attached to the particles; however, the kinetics were greatly improved, requiring half the time. Finally, St-MMA-GMA-PEGMA particles that were wetted in EL with BSA reduced the bilirubin concentration in plasma from levels that threaten the survival of critical patients to levels close to those of healthy individuals in less than 30 min. On the contrary, particles without BSA were unable to remove bilirubin from plasma. Thus, the attachment of albumin to the particles plays a key role in selectively reducing bilirubin levels.

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Protein immobilization to polystyrene via long poly(ethylene lycol) chains

Cited by 11 publications

References 6 publications

Effect of Polyelectrolyte Charge Density on the Adsorption and Desorption Behavior on Mica

Effect of Polyelectrolyte Charge Density on the Adsorption and Desorption Behavior on Mica

Grafting Poly(ethylene glycol) Epoxide to Amino-Derivatized Quartz: Effect of Temperature and pH on Grafting Density

Functionalization of Poly(styrene-co-methyl methacrylate) Particles for Selective Removal of Bilirubin

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