Protein Immobilization Using Atmospheric‐Pressure Dielectric‐Barrier Discharges: A Route to a Straightforward Manufacture of Bioactive Films

Abstract

An alternative single‐step method for biomolecule entrapment in atmospheric plasma polymers is described. Relying on our experience to fabricate organic coatings at mild plasma conditions, fluorescent proteins and an organic coating precursor were simultaneously introduced to the DBD. Fluorescence microscopy and spectrum analysis unambiguously illustrate the single‐step protein immobilization procedure using DBD. Moreover, in contrast to covalent immobilization procedures, the developed technique showed a homogeneous protein distribution in the coating.

Deposition of Hydroxyl Functionalized Films by Means of Water Aerosol‐Assisted Atmospheric Pressure Plasma

“…In recent years, aerosol‐assisted APP deposition processes emerged as novel approach to the plasma deposition of functionalized coatings . This technique allows the direct injection in APP systems of “mist” (aerosol) of pure liquid monomers, solutions, and nanoparticle suspensions.…”

In Situ Generation of Plasma-Polymer Standards by Plasma Assisted Free Radical Polymerization

“…Standards could be of various sources, either commercial if the polymers of interest are widely used or easy to produce, or from home‐made synthesis if precursors are a bit more exotic, using more or less controlled reactions. The specific case of acrylate precursors is of high interest – and will constitute the topic of the present paper – because of (a) their high reactivity towards the plasma discharge which allows both heavily cross‐linked structures to be deposited and fragile functional groups to be preserved thanks to a reduced plasma power, hence raising a growing interest into the plasma community and (b) such class of compounds readily polymerize in “wet” conditions using radical polymerization (either free or controlled, as discussed later). Reference products are then directly synthesized from the precursor used in the plasma polymerization and expected to mimic the associated plasma‐polymers.…”

Plasma Deposition of PEO‐Like Coatings with Aerosol‐Assisted Dielectric Barrier Discharges

“…Among several discharge configurations that allow to ignite cold plasmas at AP, dielectric barrier discharges (DBDs) are becoming more and more attractive for surface modification processes,23 and the interest towards surface modification AP plasmas for biomedical applications is growing 24–31. To the best of our knowledge, Nisol et al31 have published the first paper on PEO‐like films AP plasma‐deposited by using a plasma torch in two different modes, namely: AP plasma liquid deposition (APPLD), where the liquid precursor tetraethylglycoldimethylether (TEGDME) is sprayed on the substrate and then exposed to an argon plasma for activating and cross‐linking the coating, and AP plasma enhanced chemical vapours deposition (APPECVD), where TEGDME vapours are injected into the plasma with a carrier flow of argon from a heated bubbling system.…”