Protein import into chloroplasts

Soll, Jürgen

doi:10.1016/s1369-5266(02)00296-0

Cited by 186 publications

(246 citation statements)

References 75 publications

Supporting

Mentioning

241

Contrasting

Unclassified

Order By: Relevance

“…(ii) Post-translational import of proteins into chloroplasts and mitochondria facilitated by the Tic/Toc and Tim/Tom complexes, respectively. Isoforms of these translocons exist with different substrate specificity, possibly allowing the accumulation of tissue-specific plastid and mitochondrial proteomes (Lister et al, 2004;Soll and Schleiff, 2004). Regulation of protein import into chloroplasts, moreover, seems to involve sensing and reacting to the redox state of the organelle (Kü chler et al, 2002).…”

Section: Anterograde Mechanismsmentioning

confidence: 99%

Genomics-based dissection of the cross-talk of chloroplasts with the nucleus and mitochondria in Arabidopsis

Leister¹

2005

Gene

View full text Add to dashboard Cite

Post-endosymbiotic evolution of chloroplasts was characterized by a massive transfer of cyanobacterial genes to the nucleus, followed by re-routing of many of their encoded proteins. In consequence, most plastid proteins are nucleus-encoded, enabling an anterograde (nucleusto-plastid) control of the organelle. The regulation of chloroplast functions includes also cross-talk between chloroplasts and mitochondria, as well as retrograde (plastid-to-nucleus) signalling. Genetic analyses reveal that redox state, flux through the chlorophyll biosynthetic pathway, sugar sensing and reactive oxygen species contribute to retrograde signalling. However, the identity of the messenger molecule(s) remains largely unknown. Novel facets of the chloroplast -mitochondrion cross-talk have been revealed by the characterization of mitochondrial mutants affected in chloroplast properties. Studies of the nuclear chloroplast transcriptome imply the existence of at least three distinct types of transcriptional regulation: a master switch, acting in a binary mode by either inducing or repressing the same large set of genes; a ''mixed response'' with about equal numbers of up-and down-regulated genes; and mechanisms supporting the specific co-regulation of nuclear genes for photosynthesis and for plastid gene expression. The recent discovery of the latter mode of control highlights a possibly ancient route to co-ordinate chloroplast and nuclear genome expression. D

show abstract

Section: Anterograde Mechanismsmentioning

confidence: 99%

Genomics-based dissection of the cross-talk of chloroplasts with the nucleus and mitochondria in Arabidopsis

Leister¹

2005

Gene

View full text Add to dashboard Cite

show abstract

“…The standard import pathway for preproteins has been described in some detail [2]. In general, the chloroplast targeted protein, e.g.…”

Section: Introductionmentioning

confidence: 99%

Requirements for a conservative protein translocation pathway in chloroplasts

Vojta¹,

Soll²,

Bölter³

2007

FEBS Letters

Self Cite

View full text Add to dashboard Cite

The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re-export of Tic110 from the soluble intermediate stage into the inner envelope requires ATP hydrolysis, which cannot be replaced by other NTPs. Furthermore, the molecular chaperone Hsp93 seems prominently involved in the reexport pathway of Tic110, because other stromal intermediates like that of the oxygen evolving complex subunit OE33 (iOE33) en route to the thylakoid lumen interacts preferentially with Hsp70.

show abstract

“…C orrect and efficient targeting and translocation of proteins from their sites of synthesis into or across a membrane to their functional compartments is a fundamental process 1,2 . Chloroplasts contain about 3,000 proteins, but only 50-200 of them are encoded for in the plastid genome.…”

mentioning

confidence: 99%

“…Nuclear-encoded proteins are synthesized in the cytosol and post-translationally translocated across the chloroplast envelopes [1][2][3][4][5][6] . The protein import complexes located on the outer and inner envelope membranes are termed as TOC (translocon at the outer envelop membrane of chloroplast) and TIC (translocon at the inner envelop membrane of chloroplast) complexes, respectively 1,2,5,6 . The Toc159, Toc75 and Toc34 proteins form the core TOC complex and are involved in protein transport across the outer envelope [6][7][8] .…”

mentioning

confidence: 99%

“…The Toc159, Toc75 and Toc34 proteins form the core TOC complex and are involved in protein transport across the outer envelope [6][7][8] . Tic110, Tic20 and Tic21 have been suggested to function as the protein translocation channel of the inner envelope 1,2,7,8 . Several stromal proteins, including chaperones, proteases and redox regulators, are also involved in the import process 6,9,10 .…”

mentioning

confidence: 99%

See 1 more Smart Citation

LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway

Ouyang

et al. 2011

Nat Commun

View full text Add to dashboard Cite

Higher plants require chloroplasts for essential functions in photosynthesis and other important physiological processes, such as sugar, lipid and amino-acid biosynthesis. most chloroplast proteins are nuclear-encoded proteins that are synthesized in the cytosol as precursors, and imported into chloroplasts by protein translocases in the outer and inner chloroplast envelope. The imported chloroplast proteins are then translocated into or across the thylakoid membrane by four distinct pathways. However, the mechanisms by which the imported nuclear-encoded proteins are delivered to these pathways remain largely unknown. Here we show that an Arabidopsis ankyrin protein, LTD (mutation of which causes the light-harvesting chlorophyllbinding protein translocation defect), is localized in the chloroplast and using yeast two-hybrid screens demonstrate that LTD interacts with both proteins from the signal recognition particle (sRP) pathway and the inner chloroplast envelope. our study shows that LTD is essential for the import of light-harvesting chlorophyll-binding proteins and subsequent routing of these proteins to the chloroplast sRP-dependent pathway.

show abstract

Protein import into chloroplasts

Cited by 186 publications

References 75 publications

Genomics-based dissection of the cross-talk of chloroplasts with the nucleus and mitochondria in Arabidopsis

Genomics-based dissection of the cross-talk of chloroplasts with the nucleus and mitochondria in Arabidopsis

Requirements for a conservative protein translocation pathway in chloroplasts

LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway

Contact Info

Product

Resources

About