Protein impurities from cell culture dramatically impact transduction efficiency of polymer/virus hybrid vectors

Vupputuri, Sravanthi; Karode, Shishir; Neely, Brian J.; Ramsey, Joshua D.

doi:10.1016/j.jviromet.2013.04.007

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“…130 Cationic as well as anionic additives with high molecular weight can sediment with VPs onto cells in culture, which increases TE due to increased contact between VPs and cells. 131,132 VVs can also be immobilized on surfaces prior to cellular adhesion. 133,134 Chemically facilitated cell entry can also be achieved by manipulating the host cell itself.…”

Section: Physical Methodsmentioning

confidence: 99%

“…Proteins bound to VPs can increase TE due to sedimentation of heathier particles, however at the same time cellular uptake pathway is changed by larger VPs thus protein addition has to be carefully balanced. 132 This observation have far-reaching consequences for comparability of studies concerning TE, when taking batch-to-batch variability of culture medium and VP purification into account. For example, proteins from the human serum can also augment AAV transduction.…”

Section: Reviewmentioning

confidence: 99%

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