Protein interactions of SOC1 with SVP are regulated by a few crucial amino acids in flowering pathways of Brassica juncea

Li, Chaochuang; Gu, Huiying; Jiang, Wei; Zou, Congming; Wei, Dongfeng; Wang, Zhimin; Tang, Qinglin

doi:10.1007/s11738-019-2838-5

Cited by 10 publications

(7 citation statements)

References 27 publications

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“…It interacts with another SVP isoform GLYMA06G10020.3 (Figure 7B). The interaction of SVP and SOC1 is regulated by few amino acids that are highly conserved [46]. But no such interaction between SVP and SOC was detected in our analysis.…”

Section: String Analysis and Selection Of Flowering Related Candidate Genescontrasting

confidence: 63%

Identification and characterization of MADS-box gene family in pigeonpea, their wild relatives and comparative phylogenetic analysis with grain legumes.

et al. 2020

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MADS-box genes are classes of transcription factors involved in various physiological and developmental processes in plants. Here, genome wide identification of MADS-box genes was done in Cajanus cajan, identifying 102 members, classified into two different groups based on their gene structure. The gene based phylogeny of C. cajan MADS-box genes, and some grain legumes was developed to detect their gene homologs in C. cajan. The status of all these genes was analyzed in three wild relatives i.e. C. scarabaeoides, C. platycarpus and C. cajanifolius. A total of 41 MADS-box genes were found to be missing in wild type cultivars hinting towards their role in domestication and evolution. Single copy of Flowering locus C (FLC) and Short vegetative phase (SVP), while three copies of Suppressor of activation of Constans 1 (SOC1) was found to be present. One SOC1 gene i.e. CcMADS1.5 was found to be missing in all wild relatives, also forming separate clade in phylogeny, revealing its origin through duplication followed by divergence, and role in domestication. Expression profiling of major MADS-box genes involved in flowering was done in different tissues viz vegetative meristem vegetative leaf, reproductive meristem and reproductive bud. Gene based time tree of FLC and SOC1 gene dictates their divergence from Arabidopsis before 71 and 23 million year ago (mya) respectively. This study provides valuable insights into the functions, characteristics and evolution of MADS-box proteins in grain legumes with emphasis on C. cajan, which may help in further characterizing these genes.

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Section: String Analysis and Selection Of Flowering Related Candidate Genescontrasting

confidence: 63%

Identification and characterization of MADS-box gene family in pigeonpea, their wild relatives and comparative phylogenetic analysis with grain legumes.

et al. 2020

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“…This result prompted us to explore the flowering regulatory roles of the strong expansion of AP1 / SOC1 genes in ecological adaptation of orchids. Yeast two‐hybrid and bimolecular fluorescence complementation (BiFC) assays confirmed that CsSVP proteins formed homodimers and could interact with AP1 and SOC1 (Figures 4c and S14 ), suggesting more extensive protein‐binding activity as previously reported for Arabidopsis and Brassica juncea (Jang et al ., 2009 ; Lee et al ., 2007 ; Li et al ., 2019 ; Mendez‐Vigo et al ., 2013 ). These results suggested that C .…”

Section: Resultsmentioning

confidence: 99%

“…For example, in B . juncea , mutation of SOC1 C137K led to loss of protein interaction between BjuSOC1 and BjuSVP in regulating flowering time (Li et al ., 2019 ). However, we observed that the 137th amino acid of CsSOC1 that interacted with CsSVP was K (Figure S15 ).…”

Section: Resultsmentioning

confidence: 99%

The genome of Cymbidium sinense revealed the evolution of orchid traits

Yang

Gao

Wei

et al. 2021

Plant Biotechnology Journal

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Summary The Orchidaceae is of economic and ecological importance and constitutes ˜10% of all seed plant species. Here, we report a genome physical map for Cymbidium sinense, a well‐known species belonging to genus Cymbidium that has thousands of natural variation varieties of flower organs, flower and leaf colours and also referred as the King of Fragrance, which make it arose into a unique cultural symbol in China. The high‐quality chromosome‐scale genome assembly was 3.52 Gb in size, 29 638 protein‐coding genes were predicted, and evidence for whole‐genome duplication shared with other orchids was provided. Marked amplification of cytochrome‐ and photosystem‐related genes was observed, which was consistent with the shade tolerance and dark green leaves of C. sinense. Extensive duplication of MADS‐box genes, and the resulting subfunctional and expressional differentiation, was associated with regulation of species‐specific flower traits, including wild‐type and mutant‐type floral patterning, seasonal flowering and ecological adaption. CsSEP4 was originally found to positively regulate gynostemium development. The CsSVP genes and their interaction proteins CsAP1 and CsSOC1 were significantly expanded and involved in the regulation of low‐temperature‐dependent flowering. Important genetic clues to the colourful leaf traits, purple‐black flowers and volatile trait in C. sinense were also found. The results provide new insights into the molecular mechanisms of important phenotypic traits of Cymbidium and its evolution and serve as a powerful platform for future evolutionary studies and molecular breeding of orchids.

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“…SVP is a well-known member of the MADS-box gene family that negatively regulates the early stages of floral transition in Arabidopsis [ 74 , 75 ]. In particular, the expression of the SOC1 gene is inhibited by SVP during the flowering process, suggesting an interaction between them [ 76 ]. Previous studies show that the overexpression of MtSVP1 can delay flowering in Arabidopsis , while it only changes the floral organ characteristics in Medicago truncatula [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Phytohormones and Transcriptomic Profiling of the Female and Male Inflorescence Development in Manchurian Walnut (Juglans mandshurica Maxim.)

Han

Cai

et al. 2022

IJMS

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Flowers are imperative reproductive organs and play a key role in the propagation of offspring, along with the generation of several metabolic products in flowering plants. In Juglans mandshurica, the number and development of flowers directly affect the fruit yield and subsequently its commercial value. However, owing to the lack of genetic information, there are few studies on the reproductive biology of Juglans mandshurica, and the molecular regulatory mechanisms underlying the development of female and male inflorescence remain unclear. In this study, phytohormones and transcriptomic sequencing analyses at the three stages of female and male inflorescence growth were performed to understand the regulatory functions underlying flower development. Gibberellin is the most dominant phytohormone that regulates flower development. In total, 14,579 and 7188 differentially expressed genes were identified after analyzing the development of male and female flowers, respectively, wherein, 3241 were commonly expressed. Enrichment analysis for significantly enriched pathways suggested the roles of MAPK signaling, phytohormone signal transduction, and sugar metabolism. Genes involved in floral organ transition and flowering were obtained and analyzed; these mainly belonged to the M-type MADS-box gene family. Three flowering-related genes (SOC1/AGL20, ANT, and SVP) strongly interacted with transcription factors in the co-expression network. Two key CO genes (CO3 and CO1) were identified in the photoperiod pathway. We also identified two GA20xs genes, one SVP gene, and five AGL genes (AGL8, AGL9, AGL15, AGL19, and AGL42) that contributed to flower development. The findings are expected to provide a genetic basis for the studies on the regulatory networks and reproductive biology in inflorescence development for J. mandshurica.

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Protein interactions of SOC1 with SVP are regulated by a few crucial amino acids in flowering pathways of Brassica juncea

Cited by 10 publications

References 27 publications

Identification and characterization of MADS-box gene family in pigeonpea, their wild relatives and comparative phylogenetic analysis with grain legumes.

Identification and characterization of MADS-box gene family in pigeonpea, their wild relatives and comparative phylogenetic analysis with grain legumes.

The genome of Cymbidium sinense revealed the evolution of orchid traits

Characterization of Phytohormones and Transcriptomic Profiling of the Female and Male Inflorescence Development in Manchurian Walnut (Juglans mandshurica Maxim.)

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