Protein interactions with chemical vapor deposited graphene modified by substrate

Abstract

Graphene has been utilized in sensors to detect a wide range of biomolecules (e.g. glucose, DNA, antigens, enzyme activity, dopamine) using various sensing modalities (e.g. surface plasmon resonance, potentiometry, electro-impedance spectroscopy, cyclic voltammetry). However, while graphene-based biosensors have been demonstrated in many different architectures, little attention has been given to the effects of the substrate that supports the atomically thin graphene layer. In this work, we investigate protein adhesion of model small (lysozyme), medium (bovine serum albumin), and large (fibrinogen) proteins on monolayer graphene with support substrates of varying hydrophobicity and surface polarity. Ex situ adsorption is measured via ellipsometry. For Au and Si support substrates, in situ adhesion of lysozyme is measured via quartz crystal microbalance. The results indicate that not only the equilibrium attachment, but also the kinetics of interaction, can be affected by the substrate. Overall, a more hydrophobic substrate leads to a larger amount of adsorption to graphene. Moreover, the effect is only observed with monolayer graphene, where no substrate effect is observed with the addition of a second graphene layer. This work indicates that the substrate of a graphene-based biosensor is an important but currently overlooked parameter when understanding and optimizing the performance of the device. The level of non-selective protein adsorption on graphene can be independently engineered through modifying the support substrate without directly modifying the graphene itself.

Revealing the Regulation Effect of Surface Charge at Aromatic Interface to Dynamic Conformational Changes of α‐Synuclein at Early Aggregation Stage

“…[23] This suggests that the adsorption of α-syn followed the two-state adsorption model, where α-syn adsorbs to the interface in a reversible adsorption state and changes to an irreversible adsorption state through structure changes. [24] Fitted curves are shown in solid lines, and the time constants for the initial adsorption process (t 1 ) and second process (t 2 ) are 85.79 ± 8.49 s and 2349.25 ± 177.16 s for PMBA SAM, while 103.87 ± 5.95 s and 2624.14 ± 247.10 s for PATP SAM, respectively. The t 1 for PMBA SAM is smaller than that for PATP SAM, indicating stronger interactions between α-syn monomer and PMBA SAM.…”

Portable glucose sensing analysis based on laser-induced graphene composite electrode

“…8,9 Composites that incorporate graphene, conductive polymers, and metal nanoparticles offer improved performance and expand the range of applications for these materials. Graphene/conductive polymer/metal nanoparticle composites can detect a variety of compounds and molecules such as glucose, urea, protein, and DNA, [10][11][12][13][14] indicating promising prospects in the eld of biosensing.…”