Protein Kinase C and cAMP Mediated Regulation of Taurine Transport in Human Colon Carcinoma Cell Lines (HT-29 &amp; Caco-2)

Park, Sungyoun; Lee, Haemi; Park, Kun Koo; Kim, Ha Won; Park, Tae Sun

doi:10.1007/978-1-4615-0077-3_21

Cited by 2 publications

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“…L6 rat skeletal muscle myoblasts (American Type Culture Collection, Rockville, MD, USA) were seeded into 6-well plates at a density of 1.5 × 10 4 cells/well, and cultured in DMEM supplemented with 2% FBS for 13 days. Differentiated L6 cells were cultured in serum-free DMEM supplemented with 2% bovine serum albumin (BSA) for 24 hrs, and then treated with indicated concentrations of glucocorticoid (10∼1000 nM, Sigma, St. Louis, MO, USA), β-endorphin (1∼20 nM, Sigma), or IGF-1 (1∼100 nM, Sigma) for 24 hrs at 37 • C. Taurine uptake was measured in confluent monolayer cultures of L6 cells 14 days after seeding as described previously [6]. Total RNA was isolated from L6 cells treated with glucocorticoid (100 nM) or glucocorticoid (100 nM) plus IGF-1(10 nM) for 24 hrs using the Trizol reagent (Gibco-BRL, Grand Island, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

Cortisol and IGF‐1 synergistically up‐regulate taurine transport by the rat skeletal muscle cell line, L6

2004

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This study was undertaken to evaluate effects of exercise-induced hormones, cortisol, IGF-1, and beta-endorphin, on the regulation of taurine transport activity in rat skeletal myoblasts, L6 cells. Challenge of L6 cells with cortisol (100 nM) for 24 hrs resulted in a 165% increase in taurine transport activity, 220% increase in Vmax of the taurine transporter, and 55% increase in taurine transporter/ beta-actin mRNA level compared with untreated control cells. Neither IGF-1 (1 approximately 100 nM) nor beta-endorphin (1 approximately 20 nM), added in the incubation medium separately for 24 hrs, affected taurine uptake by L6 cells. However, when cells were co-treated with IGF-1 (10 nM) plus cortisol (100 nM), taurine transport activity (37% increase, p < 0.05), Vmax of the transporter (54%, p < 0.05), and taurine transporter/ beta-actin mRNA level were further increased compared to the value for cells treated with cortisol alone. These results suggest that taurine transport by skeletal muscle cells appear to be synergistically up-regulated during a prolonged exercise via elevated levels of cortisol and IGF-1 in muscle.

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Section: Methodsmentioning

confidence: 99%