Protein kinase C and intracellular calcium are involved in follicle-stimulating hormone-mediated meiotic resumption of cumulus cell-enclosed porcine oocytes in hypoxanthine-supplemented medium

Su, You-Qiang; Xia, Guoliang; Byskov, Anne Grete; Fu, Guodong; Yang, Can

doi:10.1002/(sici)1098-2795(199905)53:1<51::aid-mrd6>3.0.co;2-4

Cited by 64 publications

(41 citation statements)

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“…We observed the effect of individual PKC-activators on oocyte activation and CG exocytosis. PKC is involved in regulating the exit from metaphase II in porcine oocytes (Su et al, 1999;Shimada et al, 2001), but it is not entirely clear how PKC participates in the activation of oocytes. Our results show that oleoyl was the most effective in the activation of oocytes among the PKC-activators.…”

Section: Discussionmentioning

confidence: 99%

“…Despite oocyte activation using these chemicals, in which a high rate (over 78%) is achieved, a full cortical reaction pattern is not always observed (Su et al, 1999;Shimada et al, 2001;Petr et al, 2005). It has been shown in pigs that different events in egg activation may be unrelated to each other , leading to some conflicting results in the literature.…”

Section: Discussionmentioning

confidence: 99%

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The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

Tůmová

Romar

Petr

et al. 2013

Animal

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Nitric oxide (NO) and protein kinase C (PKC) are involved in the activation of mammalian oocytes, although their role in the exit from the metaphase II stage and cortical granule (CG) exocytosis is still not fully understood. The aim of this study was to verify whether the NO-donor together with specific PKC-activators induce the complete activation of porcine oocytes assessed as meiosis resumption and a cortical reaction. Pig maturated oocytes were treated with the NO-donor S-nitroso-N-acetylpenicillamine (SNAP, 2 mM) or PKC-activators such as phorbol-12-myristate-13-acetate (PMA, 100 nM), 1-oleoyl-2-acetyl-sn-glycerol (OAG, 400 mM) and L-a-phosphatidylinositol-3,4,5-trisphosphate dipalmitoyl heptaammonium salt (DPAM, 2 mM). To study the combined effect of NO-donor and PKC-activators, aliquots of oocytes were also incubated with SNAP (0.5 mM) together with PKC-activators at the same concentration as above (SNAP-DPAM, SNAP-OAG and SNAP-PMA groups). After in vitro maturation, an aliquot of oocytes was placed in a fresh medium without NO-donor or PKC-activators (Control group). Another aliquot of oocytes was activated by calcium ionophore A23187 (25 mM, 5 min). The results showed that 0% of the control oocytes reassumed meiosis. However, both the PKC-activators (DPAM 44.0 6 10.0%, OAG 63.3 6 1.0% and PMA 45.0 6 16.5%) as well as the NO-donor alone (48.7 6 21.0%) significantly induced exit from MII. Interestingly, the combination of PKC-activators and SNAP mainly restrained to the meiosis resumption (SNAP-OAG 0, SNAP-DPAM 17.4 6 2.5% and SNAP-PMA 38.4 6 8.5%). Control oocytes did not show a cortical reaction and the area occupied by CG reached 25.9 6 1.7%, whereas CGs were partially released after Ca 21 ionophore treatment (13.0 6 3.2%). Treatment with PKC-activators induced a cortical reaction compared with the control group (8.6 6 2.5, 6.7 6 1.9 and 0.7 6 0.4%, respectively, for DPAM, OAG and PMA groups). However, treatment with the NO-donor alone (SNAP group 17.2 6 2.2%) or combined with any PKC-activator prevented cortical reaction (SNAP-DPAM 20.7 6 2.6%, SNAP-OAG 16.7 6 2.9% or SNAP-PMA 20.0 6 2.4%). Besides, meiosis resumption was not always accompanied by a cortical reaction, indicating that these two activation events are independent. In conclusion, PKC-activators alone induce CG exocytosis to the same degree as calcium ionophore. However, an NO-donor alone or combined with PKC-activators is not able to induce a cortical reaction in pig oocytes.Keywords: protein kinase C, nitric oxide, pig oocyte, activation, cortical granules ImplicationsParthenogenetic activation of mammalian oocytes is commonly used in reproductive biotechnologies, for example, cloning using nuclear transfer, and its success depends on an adequate artificial stimulus. Nitric oxide (NO) has been described as an oocyte activator but it does not induce a cortical reaction. Protein kinases C (PKC) play an important role in the cortical reaction but the PKC cascade is not triggered after oocyte activation by an NO-donor. There is a possibilit...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

Tůmová

Romar

Petr

et al. 2013

Animal

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“…In porcine oocytes, PKC was observed to participate in the regulation of the resumption of meiotic maturation (Jung et al, 1992;Coskun and Lin, 1995;Kim and Menino, 1995;Su et al, 1999;Shimada et al, 2001). Fan et al (2002b) demonstrated a change in the localization of PKC -α, -β I , -γ in maturing porcine oocytes and after their activation.…”

Section: Which Signalling Cascades Remained Silent In No-induced Oocymentioning

confidence: 99%

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Petr¹,

Eva²,

Tůmová³

et al. 2007

CZECH J ANIM SCI

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Parthenogenetic activation of mammalian oocytes with artificial stimuli is commonly applied in various reproductive biotechniques, e.g. cloning using nuclear transfer. For this reason, many studies focus on oocyte activation in vitro. Recently we have described the activation of pig oocytes using nitric oxide. This activating stimulus is very specific in many aspects. However, it does not provide an adequate stimulus for parthenogenetic development. It was shown that nitric oxide stimulated some signalling pathways which are inactive in conventional treatments for parthenogenetic activation, e.g. the cGMP-dependent signalling cascade. On the other hand, nitric oxide does not stimulate certain signalling pathways involved in oocyte activation after calcium ionophore, e.g. the PKC signalling cascade. The aim of this review is to characterize the complex processes induced in oocytes after treatment with nitric oxide. Perspectives for further research and the application of nitric oxide for parthenogenetic activation of oocytes are outlined.

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“…In porcine oocytes, PKC was observed to participate in the regulation of the resumption of meiotic maturation (Jung et al, 1992;Coskun and Lin, 1995;Kim and Menino, 1995;Su et al, 1999;Shimada et al, 2001). Fan et al (2002b) demonstrated a change in the localization of PKC-α, -β I , -γ in maturing porcine oocytes after their activation.…”

mentioning

confidence: 97%

Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor

Petr¹,

Eva²,

Dörflerová³

et al. 2007

CZECH J ANIM SCI

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Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50µM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours). Protein kinase C (PKC) inhibitors, bisindolylmaleimide I or rottlerin, are able to inhibit parthenogenetic activation induced by calcium ionophore. The rate of activated oocytes decreased from 69% to 2% (P < 0.05) under the effect of bisindolylmaleimide I at a concentration of 0 or 20nM, respectively. The activation rate decreased from 68% to 0% (P < 0.05) under the influence of 0 or 20µM rottlerin, respectively. PKC inhibitors Go6976 or hispidin had no effect on the oocyte activation using calcium ionophore or on oocytes activated by a nitric oxide donor. The activation of oocytes by a nitric oxide donor is not significantly influenced even under the effects of bisindolylmaleimide I or rottlerin. Based on these data we can conclude that the oocyte activation induced by calcium ionophore depends on PKC, especially on PKC-δ. On the other hand, the oocyte activation induced by nitric oxide is independent of the tested isotypes of PKC.

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Protein kinase C and intracellular calcium are involved in follicle-stimulating hormone-mediated meiotic resumption of cumulus cell-enclosed porcine oocytes in hypoxanthine-supplemented medium

Cited by 64 publications

References 39 publications

The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

The effect of protein kinase C activator and nitric oxide donor on oocyte activation and cortical granule exocytosis in porcine eggs

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor

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