Protein kinases Elm1 and Sak1 of Saccharomyces cerevisiae exerted different functions under high-glucose and heat shock stresses

Wang, Lu; Xu, Yang; Jiang, Huan-Yuan; Song, Ze-Ming; Lin, Xue; Hu, Xiaoping; Li, Congfa

doi:10.1007/s00253-022-11840-2

Cited by 6 publications

(3 citation statements)

References 47 publications

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“…The supernatants of FCW, NPCW, and SC7-PCW were filtered and derivatized using an Agilent automatic on-line derivatization method for the analysis of amino acids according to the previous study [ 19 , 20 ]. The primary and secondary amino acids were reacted with O-phthalaldehyde (OPA) and fluorene methoxycarbonyl chloride (FMOC), respectively.…”

Section: Methodsmentioning

confidence: 99%

Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids

Lin

Song

Jiang

et al. 2022

Foods

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The uncontrolled natural pre-fermentation process of coconut water represents great hidden safety hazards, unstable production, and impact on the quality of nata de coco–the trade name of bacterial cellulose (BC) in food industry. In this study, BC production from Komagataeibacter nataicola Q2 was conducted in the media of coconut water (50%, v/v) pre-fermented by 11 coconut-sourced yeast strains in static. Results suggested that coconut water pre-fermented by different yeast strains had varied effects on the production of BC. Compared with the use of fresh coconut water, the use of coconut water pre-fermented by Saccharomyces cerevisiae SC7 increased the BC yield by 165%. Both natural pre-fermentation and SC7 pre-fermentation altered the concentrations of amino acids in fresh coconut water. The addition of selected amino acids aspartic acid, glutamic acid, serine, methionine, threonine, isoleucine, phenylalanine, and proline at different concentrations had varied effects on the production of BC. The yield of BC was the highest when adding 3.0% (w/v) methionine. Moreover, adding 3.0% methionine allowed the production of BC with larger loops of looser aggregated microfibers, increased the crystallinity of BC from 64.8% to 69.4%, but decreased the temperature of maximum weight loss rate, hardness, and adhesiveness from 223 °C, 8.68 kg, and 92.8 g.sec to 212 °C, 7.01 kg, and 58.5 g.sec, respectively, in the test condition.

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Section: Methodsmentioning

confidence: 99%

Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids

Lin

Song

Jiang

et al. 2022

Foods

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“…Activated Snf1 phosphorylates Kog1, which is located in nearby Ser491 and Ser494, leading to Kog1 being separated from TORC1. This leads to lower activation of TORC1 ( Figure 2 ) [ 37 ]. In fact, the pathway inactivates TORC1 to ensure that cells stay in the quiescent stage and survive under starvation conditions by increasing its activation threshold [ 38 ].…”

Section: Upstream Signals Of Torc1 In Yeast Fungimentioning

confidence: 99%

TORC1 Signaling in Fungi: From Yeasts to Filamentous Fungi

Wang

Zheng

et al. 2023

Microorganisms

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Target of rapamycin complex 1 (TORC1) is an important regulator of various signaling pathways. It can control cell growth and development by integrating multiple signals from amino acids, glucose, phosphate, growth factors, pressure, oxidation, and so on. In recent years, it has been reported that TORC1 is of great significance in regulating cytotoxicity, morphology, protein synthesis and degradation, nutrient absorption, and metabolism. In this review, we mainly discuss the upstream and downstream signaling pathways of TORC1 to reveal its role in fungi.

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“…Elucidation of the thermotolerance mechanism is helpful for the breeding of thermotolerant Y. lipolytica . At present, what we have known about the defense mechanism of yeast upon thermal stress predominantly comes from the study of Saccharomyces cerevisiae (Caspeta et al 2014 ; Gao et al 2016 ; Muhlhofer et al 2019 ; Wang et al 2022 ). For the erythritol-producing Y. lipolytica , the central carbon metabolism and amino acid metabolism were found to be involved in the thermal stress response at a transcriptional level, while ATP, thiamine, and trehalose were critical molecules for cells to grow under conditions of high temperature (Qiu et al 2021 ; Celinska 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution

Xia,

Chen,

Liu

et al. 2024

Appl Microbiol Biotechnol

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Elucidation of the thermotolerance mechanism of erythritol-producing Yarrowia lipolytica is of great significance to breed robust industrial strains and reduce cost. This study aimed to breed thermotolerant Y. lipolytica and investigate the mechanism underlying the thermotolerant phenotype. Yarrowia lipolytica HT34, Yarrowia lipolytica HT36, and Yarrowia lipolytica HT385 that were capable of growing at 34 °C, 36 °C, and 38.5 °C, respectively, were obtained within 150 days (352 generations) by adaptive laboratory evolution (ALE) integrated with 60Co-γ radiation and ultraviolet ray radiation. Comparative genomics analysis showed that genes involved in signal transduction, transcription, and translation regulation were mutated during adaptive evolution. Further, we demonstrated that thermal stress increased the expression of genes related to DNA replication and repair, ceramide and steroid synthesis, and the degradation of branched amino acid (BCAA) and free fatty acid (FFA), while inhibiting the expression of genes involved in glycolysis and the citrate cycle. Erythritol production in thermotolerant strains was remarkably inhibited, which might result from the differential expression of genes involved in erythritol metabolism. Exogenous addition of BCAA and soybean oil promoted the growth of HT385, highlighting the importance of BCAA and FFA in thermal stress response. Additionally, overexpression of 11 out of the 18 upregulated genes individually enabled Yarrowia lipolytica CA20 to grow at 34 °C, of which genes A000121, A003183, and A005690 had a better effect. Collectively, this study provides novel insights into the adaptation mechanism of Y. lipolytica to thermal stress, which will be conducive to the construction of thermotolerant erythritol-producing strains. Key points • ALE combined with mutagenesis is efficient for breeding thermotolerant Y. lipolytica • Genes encoding global regulators are mutated during thermal adaptive evolution • Ceramide and BCAA are critical molecules for cells to tolerate thermal stress

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Protein kinases Elm1 and Sak1 of Saccharomyces cerevisiae exerted different functions under high-glucose and heat shock stresses

Cited by 6 publications

References 47 publications

Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids

Production of Bacterial Cellulose in the Medium with Yeasts Pre-Fermented Coconut Water or with Addition of Selected Amino Acids

TORC1 Signaling in Fungi: From Yeasts to Filamentous Fungi

Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution

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