Protein-liposome interactions: the impact of surface charge and fluidisation effect on protein binding

Triantafyllopoulou, Efstathia; Pippa, Νatassa; Demetzos, Costas

doi:10.1080/08982104.2022.2071296

Cited by 7 publications

(4 citation statements)

References 66 publications

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“…Generally, the incorporation of and increase in DOPC into the membrane lead to more fluid bilayers for all the hybrid systems investigated (Figure 4). The pretransition is absent in all cases due to modifications in the mobility of the polar head group region [38,46], while the peak width at half height is higher and Tm is decreasing, as is the enthalpy. The double bonds of DOPC in the center of the aliphatic chains favor the decrease in van der Waals interactions in the hydrophobic interior, permitting conformational freedom [32].…”

Section: The Influence Of Unsaturated Dopc In Hybrid Bilayersmentioning

confidence: 92%

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

et al. 2023

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Hybrid nanoparticles have gained a lot of attention due to their advantageous properties and versatility in pharmaceutical applications. In this perspective, the formation of novel systems and the exploration of their characteristics not only from a physicochemical but also from a biophysical perspective could promote the development of new nanoplatforms with well-defined features. In the current work, lipid/copolymer bilayers were formed in different lipid to copolymer ratios and examined via differential scanning calorimetry as a preformulation study to decipher the interactions between the biomaterials, followed by nanostructure preparation by the thin-film hydration method. Physicochemical and toxicological evaluations were conducted utilizing light scattering techniques, fluorescence spectroscopy, and MTS assay. 1,2-dioctadecanoyl-sn-glycero-3-phosphocholine (DSPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) in different weight ratios were the chosen lipids, while a linear random copolymer with pH- and thermoresponsive properties comprised of oligo (ethylene glycol) methyl ether methacrylate (OEGMA) and 2-(diisopropylamino) ethyl methacrylate (DIPAEMA) in different ratios was used. According to our results, non-toxic hybrid nanosystems with stimuli-responsive properties were successfully formulated, and the main parameters influencing their overall performance were the hydrophilic/hydrophobic balance, lipid to polymer ratio, and more importantly the random copolymer topology. Hopefully, this investigation can promote a better understanding of the factors affecting the behavior of hybrid systems.

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Section: The Influence Of Unsaturated Dopc In Hybrid Bilayersmentioning

confidence: 92%

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

et al. 2023

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“… 43 − 46 The protein corona formation and its results in vivo are a multifactorial concept that is affected by many parameters, including size and chemical composition. 42 , 47 − 50 We are convinced that the random architecture of the copolymers utilized in this study, as well as the hydrophobic-to-hydrophilic balance, have a great impact on protein adsorption, and to the best of our knowledge, this is the first time that this has been reported.…”

Section: Resultsmentioning

confidence: 66%

“…Generally, the OEGMA chains on the exterior of the hybrid nanoparticles could ameliorate the in vivo behavior of the hybrid systems and impart nonfouling properties to the nanoparticles. However, the length and density of the OEGMA chains play a crucial role in their configuration and thus their functionality. − The protein corona formation and its results in vivo are a multifactorial concept that is affected by many parameters, including size and chemical composition. ,− We are convinced that the random architecture of the copolymers utilized in this study, as well as the hydrophobic-to-hydrophilic balance, have a great impact on protein adsorption, and to the best of our knowledge, this is the first time that this has been reported.…”

Section: Resultsmentioning

confidence: 66%

Deciphering the Lipid-Random Copolymer Interactions and Encoding Their Properties to Design a Hybrid System

Triantafyllopoulou,

Forys,

Perinelli

et al. 2024

Langmuir

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Lipid/copolymer colloidal systems are deemed hybrid materials with unique properties and functionalities. Their hybrid nature leads to complex interfacial phenomena, which have not been fully encoded yet, navigating their properties. Moving toward in-depth knowledge of such systems, a comprehensive investigation of them is imperative. In the present study, hybrid lipid/copolymer structures were fabricated and examined by a gamut of techniques, including dynamic light scattering, fluorescence spectroscopy, cryogenic transmission electron microscopy, microcalorimetry, and high-resolution ultrasound spectroscopy. The biomaterials that were mixed for this purpose at different ratios were 1,2-dioctadecanoyl-sn-glycero-3-phosphocholine and four different linear, statistical (random) amphiphilic copolymers, consisting of oligo(ethylene glycol) methyl ether methacrylate as the hydrophilic comonomer and lauryl methacrylate as the hydrophobic one. The colloidal dispersions were studied for lipid/copolymer interactions regarding their physicochemical, morphological, and biophysical behavior. Their membrane properties and interactions with serum proteins were also studied. The aforementioned techniques confirmed the hybrid nature of the systems and the location of the copolymer in the structure. More importantly, the random architecture of the copolymers, the hydrophobic-to-hydrophilic balance of the nanoplatforms, and the lipid-to-polymer ratio are highlighted as the main design-influencing factors. Elucidating the lipid/copolymer interactions would contribute to the translation of hybrid nanoparticle performance and, thus, their rational design for multiple applications, including drug delivery.

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“…Nowadays, the polymer community has been focused on the synthesis of polymeric biomaterials in order to treat such diseases. Nonviral vectors based on cationic polymers [ 6 , 7 , 8 , 9 , 10 ] and lipids [ 11 , 12 , 13 , 14 ] have been proven to be efficient carriers in gene therapy strategies. Nevertheless, the utilization of cationic copolymers as gene-delivery vehicles has some remarkable advantages in comparison with the use of lipids.…”

Section: Introductionmentioning

confidence: 99%

Novel Multi-Responsive Hyperbranched Polyelectrolyte Polyplexes as Potential Gene Delivery Vectors

et al. 2023

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In this work, we investigate the complexation behavior of poly(oligo(ethylene glycol)methyl methacrylate)-co-poly(2-(diisopropylamino)ethyl methacrylate), P(OEGMA-co-DIPAEMA), hyperbranched polyelectrolyte copolymers, synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization, with short-linear DNA molecules. The synthesized hyperbranched copolymers (HBC), having a different chemical composition, are prepared in order to study their ability to bind with a linear nucleic acid at various N/P ratios (amine over phosphate groups). Specifically, the three pH and thermo-responsive P(OEGMA-co-DIPAEMA) hyperbranched copolymers were able to form polyplexes with DNA, with dimensions in the nanoscale. Using several physicochemical methods, such as dynamic and electrophoretic light scattering (DLS, ELS), as well as fluorescence spectroscopy (FS), the complexation process and the properties of formed polyplexes were explored in response to physical and chemical stimuli such as temperature, pH, and ionic strength. The mass and the size of polyplexes are shown to be affected by the hydrophobicity of the copolymer utilized each time, as well as the N/P ratio. Additionally, the stability of polyplexes in the presence of serum proteins is found to be excellent. Finally, the multi-responsive hyperbranched copolymers were evaluated regarding their cytotoxicity via in vitro experiments on HEK 293 non-cancerous cell lines and found to be sufficiently non-toxic. Based on our results, these polyplexes could be useful candidates for gene delivery and related biomedical applications.

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Protein-liposome interactions: the impact of surface charge and fluidisation effect on protein binding

Cited by 7 publications

References 66 publications

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

Development of Hybrid DSPC:DOPC:P(OEGMA950-DIPAEMA) Nanostructures: The Random Architecture of Polymeric Guest as a Key Design Parameter

Deciphering the Lipid-Random Copolymer Interactions and Encoding Their Properties to Design a Hybrid System

Novel Multi-Responsive Hyperbranched Polyelectrolyte Polyplexes as Potential Gene Delivery Vectors

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