Protein manipulation with insulator-based dielectrophoresis and direct current electric fields

Lapizco‐Encinas, Blanca H.; Ozuna-Chacón, Sandra; Rito‐Palomares, Marco

doi:10.1016/j.chroma.2008.05.077

Cited by 122 publications

(149 citation statements)

References 35 publications

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“…This is in contrary to recently published results of the Lapizco-Encinas group on DC iDEP of bovine serum albumin. 29 While this group reports on protein particle trapping in a DC iDEP device at similar solution conductivities, we remark that electric fields and gradients thereof are much larger in our device. Comparing their data 29 with ours 25 we conclude that even at an order of magnitude higher electric fields and $2-3 order of magnitude larger rE 2 DC iDEP streaming is apparent, both theoretically and experimentally (this paper).…”

Section: A Dependence On Phmentioning

confidence: 97%

“…29 While this group reports on protein particle trapping in a DC iDEP device at similar solution conductivities, we remark that electric fields and gradients thereof are much larger in our device. Comparing their data 29 with ours 25 we conclude that even at an order of magnitude higher electric fields and $2-3 order of magnitude larger rE 2 DC iDEP streaming is apparent, both theoretically and experimentally (this paper). This is remarkable, as we even employed a larger protein (150 kDa IgG compared to 60 kDa bovine serum albumin 29 ) from which we would expect a larger DEP force according to classical theory.…”

Section: A Dependence On Phmentioning

confidence: 97%

“…However, the manipulation of proteins by DEP has recently been demonstrated. 14,[26][27][28][29][30][31] As anticipated, high field strengths and gradients are required while DEP under DC and AC conditions was reported. Interestingly, proteins with molecular weight smaller than 100 kDa were successfully accumulated on electrodes, 14,26 carbon nanotubes, 27 within droplets, 28 and using nanopipettes.…”

Section: Introductionmentioning

confidence: 99%

“…19,[26][27][28][29][30][31][32] This is not only due to the particular special arrangements at the microscale used to generate high electric field gradients, but also a consequence of the solvent conditions and intrinsic protein properties. Moreover, a generic theoretical model predicting protein polarizability and accounting for the large protein variety has not been developed yet.…”

Section: Introductionmentioning

confidence: 99%

“…19 H€ olzel et al showed the DEP induced trapping of the large multimeric protein R-phycoerythrin at metal electrode gaps; 30 whereas Bakewell et al investigated frequency dependent DEP behavior of avidin. 31 Recently, an iDEP device 29 as well as a constriction device 32,33 was employed to trap proteins.…”

Section: Introductionmentioning

confidence: 99%

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Tuning direct current streaming dielectrophoresis of proteins

et al. 2012

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Dielectrophoresis (DEP) of biomolecules has large potential to serve as a novel selectivity parameter for bioanalytical methods such as (pre)concentration, fractionation, and separation. However, in contrast to well-characterized biological cells and (nano)particles, the mechanism of protein DEP is poorly understood, limiting bioanalytical applications for proteins. Here, we demonstrate a detailed investigation of factors influencing DEP of diagnostically relevant immunoglobulin G (IgG) molecules using insulator-based DEP (iDEP) under DC conditions. We found that the pH range in which concentration of IgG due to streaming iDEP occurs without aggregate formation matches the pH range suitable for immunoreactions. Numerical simulations of the electrokinetic factors pertaining to DEP streaming in this range further suggested that the protein charge and electroosmotic flow significantly influence iDEP streaming. These predictions are in accordance with the experimentally observed pH-dependent iDEP streaming profiles as well as the determined IgG molecular properties. Moreover, we observed a transition in the streaming behavior caused by a change from positive to negative DEP induced through micelle formation for the first time experimentally, which is in excellent qualitative agreement with numerical simulations. Our study thus relates molecular immunoglobulin properties to observed iDEP, which will be useful for the future development of protein (pre)concentration or separation methods based on DEP.

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Section: A Dependence On Phmentioning

confidence: 97%

Section: A Dependence On Phmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tuning direct current streaming dielectrophoresis of proteins

et al. 2012

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Dielectrophoretic Studies of Bioparticles

2017

Dielectrophoresis

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Development of dielectrophoresis separator with an insulating porous membrane using DC‐Offset AC Electric Fields

Hakoda

2016

Biotechnology Progress

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Our previous studies revealed that the dielectrophoresis method is effective for separating cells having different dielectric properties. The purpose of this study was to evaluate the separation characteristics of two kinds of cells by direct current (DC) voltage offset/alternating current (AC) voltage using an insulating porous membrane dielectrophoretic separator. The separation device gives dielectrophoretic (DEP) force and electrophoretic (EP) force to dispersed particles by applying the DC-offset AC voltage. This device separates cells of different DEP properties by adopting a structure in which only the parallel plate electrodes and the insulating porous membrane are disposed in the flow path through which the cell-suspension flows. The difference in the retention ratios of electrically homogeneous 4.5 μm or 20.0 μm diameter standard particles was a maximum of 82 points. Furthermore, the influences of the AC voltage or offset voltage on the retention ratios of mouse hybridoma 3-2H3 cells and horse red blood cells (HRBC) were investigated. The difference in the retention ratio of the two kinds of cells was a maximum of 56 points. The separation efficiency of this device is expected to be improved by changing the device shape, number of pores, and pore placement. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1292-1300, 2016.

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Protein manipulation with insulator-based dielectrophoresis and direct current electric fields

Cited by 122 publications

References 35 publications

Tuning direct current streaming dielectrophoresis of proteins

Tuning direct current streaming dielectrophoresis of proteins

Dielectrophoretic Studies of Bioparticles

Development of dielectrophoresis separator with an insulating porous membrane using DC‐Offset AC Electric Fields

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