Protein metabolism in the liver and white muscle is associated with feed efficiency in Chinook salmon (Oncorhynchus tshawytscha) reared in seawater: Evidence from proteomic analysis

Excessive level of dietary insect protein negatively changed growth metabolomic and transcriptomic profiles of largemouth bass (Micropterus salmoides)

“…The transcriptomic data reveal that feeding on a high HI meal diet induced expression of the genes responsible for protein synthesis, proteolysis, protein localization and transport, suggesting an enhanced protein turnover rate. Similar to the present study, enrichment of protein synthesis and catabolic processes were found in other omics studies in less feed efficient chinook salmon ( Oncorhynchus tshawytscha ) ( Esmaeili et al., 2022 , 2023 ). Enhanced protein synthesis and degradation are energy demanding for the cells, which may disturb physiological functions of the liver.…”

Physiological Benefits of Dietary Lysophospholipid Supplementation in a Marine Fish Model: Deep Analyses of Modes of Action

“…Proteomics is widely used to understand fish physiology, as this technique can show differential expression of identified proteins at various stages of fish development and under different conditions of feeding, stress, or disease, thereby providing a holistic overview of several functions in fish metabolism [32]. Here, using shotgun proteomics, we identified a high number of proteins (4850 and 4984 for the intestine and liver, respectively), like other studies using shotgun approaches [33][34][35] which were greater than those of other studies using 2D proteomics in fish tissues [19,36]. Next, we used GO enrichment analysis for the DEPs, similar to recent proteomic evaluations in fish [35,37], mainly searching for the biological processes and Reactome pathways affected by dietary LPLs.…”

“…Here, using shotgun proteomics, we identified a high number of proteins (4850 and 4984 for the intestine and liver, respectively), like other studies using shotgun approaches [33][34][35] which were greater than those of other studies using 2D proteomics in fish tissues [19,36]. Next, we used GO enrichment analysis for the DEPs, similar to recent proteomic evaluations in fish [35,37], mainly searching for the biological processes and Reactome pathways affected by dietary LPLs. According to the functional enrichment analyses, the addition of LPLs to the diet affected enterocyte function in two principal ways: cellular organization (GO:0016043, "cellular component organization", 70 DEPs), a process "that results in the assembly, arrangement of constituent parts, or disassembly of a cellular component" (GO strict definition), and cellular transport (GO:0006810, transport, 57 DEPs), which is "the directed movement of substances or cellular components (such as complexes and organelles) into, out of or within a cell, or between cells" (GO adapted definition).…”

Lotus Elixir: A melody of gene expression variation in Oncorhynchus mykiss tissues

“…The higher activity of AST and ALT enzymes in the muscle indicates the synthesis of pyruvate and oxaloacetate which are involved in the synthesis of non-essential amino acids required for growth. Amino acid catabolism occurs mostly in the liver, resulting in the formation of 50-99 percent ammonia (Esmaeili et al, 2022). The use of lotus leaf extract resulted in a substantial decrease in liver AST and ALT activity.…”