Protein Metabolism of Parenterally Fed Neonates: Combined 13c and 15n Studies

Pencharz, Paul B.; Beesley, J; Canagarayar, U; Aerde, John Van; Renner, Jordan B.; Sauer, Pieter J. J.; Wesson, DE; Swyer, Paul R.

doi:10.1203/00006450-198404001-00688

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, we are currently studying postsurgical neonates using both 15N glycine and 13C leucine. Preliminary results of these studies have been reported in abstract form (13) and do not show any significant differences in the estimate of whole body amino N flux obtained from the two different amino acids and labels.…”

Section: * Sternal Cleft Repair and Ligation Of Patent Ductus Arteriomentioning

confidence: 95%

The Effects of Surgery on the Nitrogen Metabolism of Parenterally Fed Human Neonates

Duffy

Pencharz

1986

Pediatr Res

View full text Add to dashboard Cite

ABSTRACT. Nitrogen retention and rates of whole body whole body protein synthes~s, we hypothesized that giving inamino nitrogen flux, protein synthesis, and breakdown were creased amounts of exogenous amino acids as precursor might measured in 18 neonates during the 72 h immediately reverse this process and improve net prot~in synthesis. We therepostsurgery. The infants were all intravenously fed with a fore studied neonates receiving either 2.5 g or 4.0 g/kg/day of crystalline amino acid source (Vamin), glucose, and Intra-amino acids immediately postoperatively. lipid The infants were divided into two groups based on amino acid intake: either 2.3 S D 0.4 g or 3.9 S D 0.5 g/kg/ day. Nonprotein energy intakes were similar and averaged MATERIALS AND METHODS g1 kcal/kg/day-A (n = 11) retained 145 SD 11°Eighteen newborn infants were studied in the Neonatal Intenmg N/kg/day, group (n = 7, retained 315 SD 93 sive Care Unit of The Montreal Children's Hospital. The subjects mg N/kg/day ( p < 0-001)-There were differences seen were divided randomly into two groups on the basis of the level in flux, synthesis or breakdown. However, group B had of amino acid received. The clinical characteristics of the two significantly higher net protein synthesis rates (synthesis-diet are shown in ~~b l~ 1. ~1 1 infants received amino breakdown) (P < 0.01). The improved nitrogen utilization acid/glucose solutions with complete mineral and vitamin addiin group was achieved principally by a in tions similar to those we have previously reported (6). The amino endogenous protein breakdown. There were no differences acid source chosen was a crystalline amino acid mixture patbetween the two groups in urinary creatinine or 3-methyl-terned on egg albumin (vamin; vitrurn co., stockholm, swehistidine excretion. Since these two parameters reflect den). ~h~ amino acid glucose solutions were infused in combiskeletal muscle turnover the differences between groups in nation with a 10% soy bean lipid emulsion (~~~~~l i~i d vitrum nitrogen retention and turnover appear to be mediated Co.). The studies were approved by the Human Experimentation Committee of The Montreal Children's Hospital and informed, Abbreviation written consent was obtained from the parents. The infants were N, nitrogen randomly allocated to one of the two total parenteral nutrition regimes, either 2.5 g amino acid or 4 g amino acid/kg/day. Parenteral nutrition was started within 24 h postoperatively. Seventy-two-hour N balance, whole body protein turnover, and urine 3-methylhistidine excretion studies were canied out also It has been known for many years that surgery results in an starting within 24 h after surgery. Our balance techniques have increased urinary urea excretion and often a negative N balance. been previously described (7). All studies were performed with Several investigators using isotopically labeled amino acids have the neonate in a standard servocontrolled incubator. The surgical shown that this "catabolic" loss of nitrogen appeared to be a procedures the infants ...

show abstract

Section: * Sternal Cleft Repair and Ligation Of Patent Ductus Arteriomentioning

confidence: 95%

The Effects of Surgery on the Nitrogen Metabolism of Parenterally Fed Human Neonates

Duffy

Pencharz

1986

Pediatr Res

View full text Add to dashboard Cite

show abstract

The effect of feeding route (IV or oral) on the protein metabolism of the neonate

Duffy¹,

Pencharz²

1986

The American Journal of Clinical Nutrition

View full text Add to dashboard Cite

We have examined the effect of the route of feeding (intravenous versus enteral) on the protein metabolism of postsurgical human neonates. Twelve infants, birth weight 2.5 +/- 0.2 kg, gestational age 38 +/- 1 wk, were studied. The IV study was carried out 1-4 days after surgery at a postnatal age of 14 days and a weight of 2.6 +/- 0.2 kg. The repeat (oral) study was carried out 16 days later. Protein intakes were similar during both studies (2.7 g/kg/d). Energy intakes were within the requirement range for age and feeding route and were: IV, 85 +/- 4 kcal/kg/d; oral, 111 +/- 7 kcal/kg/d. Whole body protein metabolism was studied using a continuous infusion of 15N-glycine. Amino nitrogen flux, protein synthesis, and breakdown were 40% higher during the enteral than the IV studies (p less than 0.001). Skeletal muscle degradation was investigated by measuring urinary excretion of creatinine and N-T-methylhistidine. No differences were detected due to feeding route. We suggest that the differences seen in whole body protein turnover rates reflect the rapid growth and development of the gut in the enterally (rather than the IV) fed infant.

show abstract

Protein Metabolism of Parenterally Fed Neonates: Combined 13c and 15n Studies

Cited by 2 publications

References 0 publications

The Effects of Surgery on the Nitrogen Metabolism of Parenterally Fed Human Neonates

The Effects of Surgery on the Nitrogen Metabolism of Parenterally Fed Human Neonates

The effect of feeding route (IV or oral) on the protein metabolism of the neonate

Contact Info

Product

Resources

About