Protein Microarray Substrates with High Interfacial Area Using Silica Colloidal Crystals

Wirth, Mary J.; Smith, Corey M.; Scott, Joshua; Rice, Michael E.

doi:10.1021/bk-2011-1062.ch009

Cited by 1 publication

(1 citation statement)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hollow silica nanoparticles (HSNs) are a kind of functional materials broadly applied in high-efficiency adsorbents, , drug-delivery carriers, − and molecular imaging , with the advantage of a large specific surface area as well as an adjustable pore morphology. In particular, silicon-based materials with versatile surface chemistry and low inherent fluorescence have been extensively applied in the fields of biosensing or biological detection. − In this work, we constructed a kind of nanoparticle-based 3D protein microarray in which a monolayer of HSNs was attached to an activated COC substrate with high transparency and low inherent fluorescence. As shown in Scheme , the poly[3-(trimethoxysilyl)propyl methacrylate] (PTMSPMA) brush was first photografted onto the surface of the pristine COC film.…”

Section: Introductionmentioning

confidence: 99%

Covalently Attaching Hollow Silica Nanoparticles on a COC Surface for the Fabrication of a Three-Dimensional Protein Microarray

Yan

Zhao

et al. 2022

Biomacromolecules

View full text Add to dashboard Cite

Compared to traditional two-dimensional (2D) biochips, three-dimensional (3D) biochips exhibit the advantages of higher probe density and detection sensitivity due to their designable surface microstructure as well as enlarged surface area. In the study, we proposed an approach to prepare a 3D protein chip by deposition of a monolayer of functionalized hollow silica nanoparticles (HSNs) on an activated cyclic olefin copolymer (COC) substrate. First, the COC substrate was chemically modified through the photografting technique to tether poly[3-(trimethoxysilyl) propyl methacrylate] (PTMSPMA) brushes on it. Then, a monolayer of HSNs was deposited on the modified COC and covalently attached via a condensation reaction between the hydrolyzed pendant siloxane groups of PTMSPMA and the Si–OH groups of HSNs. The roughness of the COC substrate significantly increased to 50.3 nm after depositing a monolayer of HSNs (ranging from 100 to 700 nm), while it only caused a negligible reduction in the light transmittance of COC. The HSN-modified COC was further functionalized with epoxide groups by a silane coupling agent for binding proteins. Immunoglobulin G could be effectively immobilized on this substrate with the highest immobilization efficiency of 75.2% and a maximum immobilization density of 1.236 μg/cm2, while the highest immobilization efficiency on a 2D epoxide group-modified glass slide was only 57.4%. Moreover, immunoassay results confirmed a competitive limit of detection (LOD) (1.06 ng/mL) and a linear detection range (1–100 ng/mL) of the 3D protein chip. This facile and effective approach for fabricating nanoparticle-based 3D protein microarrays has great potential in the field of biorelated detection.

show abstract