Protein Microarray Technologies for Detection and Identification of Bacterial and Protein Analytes

Wingren, Christer; Borrebaeck, Carl

doi:10.1007/978-0-387-75113-9_26

Cited by 2 publications

(1 citation statement)

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“…In recent years, surfaces featuring micropatterns of biomolecules, particularly proteins, have seen a surge of interest. They have found multiple applications in biomedical research such as microarrays (Macbeath and Schreiber, 2000; Allison et al, 2006; Wingren and Borrebaeck, 2007), proteomics (Haab, 2005; Wingren and Borrebaeck, 2008), and biomimetic sensors (Mujahid et al, 2013). Furthermore, cell-scale microstructured biointerfaces have been used to manipulate cell shape and organization to study endocytosis (Tan et al, 2015), cell polarization and proliferation (Chen et al, 1997; Théry, 2010), host-pathogen interactions (March et al, 2015) as well as stem cell differentiation (Sykova and Forostyak, 2013; Castaño et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns

Lindner

Tresztenyak²,

Fülöp

et al. 2019

Front. Chem.

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Protein micropatterning has become an important tool for many biomedical applications as well as in academic research. Current techniques that allow to reduce the feature size of patterns below 1 μm are, however, often costly and require sophisticated equipment. We present here a straightforward and convenient method to generate highly condensed nanopatterns of proteins without the need for clean room facilities or expensive equipment. Our approach is based on nanocontact printing and allows for the fabrication of protein patterns with feature sizes of 80 nm and periodicities down to 140 nm. This was made possible by the use of the material X-poly(dimethylsiloxane) (X-PDMS) in a two-layer stamp layout for protein printing. In a proof of principle, different proteins at various scales were printed and the pattern quality was evaluated by atomic force microscopy (AFM) and super-resolution fluorescence microscopy.

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Section: Introductionmentioning

confidence: 99%

A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns

Lindner

Tresztenyak²,

Fülöp

et al. 2019

Front. Chem.

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High-throughput proteomics using antibody microarrays: an update

Borrebaeck¹,

Wingren²

2007

Expert Review of Molecular Diagnostics

111

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Antibody-based microarrays are a rapidly emerging technology that has advanced from the first proof-of-concept studies to demanding serum protein profiling applications during recent years, displaying great promise within disease proteomics. Miniaturized micro- and nanoarrays can be fabricated with an almost infinite number of antibodies carrying the desired specificities. While consuming only minute amounts of reagents, multiplexed and ultrasensitive assays can be performed targeting high- as well as low-abundance analytes in complex nonfractionated proteomes. The microarray images generated can then be converted into protein expression profiles or protein atlases, revealing a detailed composition of the sample. The technology will provide unique opportunities for fields such as disease diagnostics, biomarker discovery, patient stratification, predicting disease recurrence and drug target discovery. This review describes an update of high-throughput proteomics, using antibody-based microarrays, focusing on key technological advances and novel applications that have emerged over the last 3 years.

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Protein Microarray Technologies for Detection and Identification of Bacterial and Protein Analytes

Cited by 2 publications

References 69 publications

A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns

A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns

High-throughput proteomics using antibody microarrays: an update

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