Protein microarray technology

Oligonucleotide Microarray Technology and its Application to Mycobacterium avium subsp. paratuberculosis Research: A Review

“…Today, various types of microarrays are utilised for the analysis of different target material, such as DNA [24][25][26], cDNA [27,28], RNA [29], PCR products [30], proteins [31][32][33] and other materials allowing quantitative analysis. Common types of microarrays include oligonuceotide arrays, cDNA arrays, protein and tissue arrays [20].…”

Progress in fabricating arrays of soft spherical vessels on mesoscale with spatial control

“…Particularly, the biocompatible membranes of the liposomes can provide an optimal microenvironment for the membrane proteins to prevent them from denaturing, which is not very easy to fulfill for other carriers such as the supported lipid bilayers. Therefore, one of the promising applications of the liposome arrays is to fabricate protein chips [27][28][29][30] which can be utilized as diagnostic tools or biosensors.…”

“…Binding of such a vessel onto a surface is of scientific significance because it may provide a confined biomimetic environment for studying the single-molecular behavior of biomolecules such as DNA, RNA and proteins [22][23][24][25][26] . Accordingly, integrating these vessels into a microarray system could find potential applications in protein chips [27][28][29][30] , sensors [31][32][33][34] , microanalyses [35] , and microreactors [36,37] as well.…”

Area-scaling of interferometric and fluorescent detection of protein on antibody microarrays

“…Currently, array detection methods include traditional fluorescence (Nagl et al, 2005;Park et al, 2006;Templin et al, 2002) and label-free techniques such as the BioCD (Varma et al, 2004a,b;Wang et al, 2007;Zhao et al, 2007), surface plasmon resonance (Boozer et al, 2006;Homola et al, 1999) and imaging ellipsometry (Chamritski et al, 2007). Among these approaches, the BioCD utilizes local interferometry to detect protein mass and realize label-free protein detection, and the BioCD is compatible with fluorescence detection.…”