Protein microarray technology

Templin, Markus F.; Stoll, Dieter; Schrenk, Monika; Traub, Petra C.; Vöhringer, Christian; Joos, Thomas

doi:10.1016/s0167-7799(01)01910-2

Cited by 514 publications

(310 citation statements)

References 49 publications

Supporting

Mentioning

305

Contrasting

Unclassified

Order By: Relevance

“…98,100 Engineered polypeptides hybridized with functional synthetic molecules could be used as heterofunctional building blocks in molecular electronics, ferroelectrics, and photonics. With the aid of genetic-engineering tools, spacing and orientation control of the peptides-which have been major challenges of these fields 89,90,97−100 -can be overcome for desired effects.…”

Section: Prospectsmentioning

confidence: 99%

Molecular Biomimetics: Genetic Synthesis, Assembly, and Formation of Materials Using Peptides

Tamerler¹,

Sarıkaya²

2008

MRS Bull.

View full text Add to dashboard Cite

In nature, the molecular-recognition ability of peptides and, consequently, their functions are evolved through successive cycles of mutation and selection. Using biology as a guide, it is now possible to select, tailor, and control peptide-solid interactions and exploit them in novel ways. Combinatorial mutagenesis provides a protocol to genetically select short peptides with specific affinity to the surfaces of a variety of materials including metals, ceramics, and semiconductors. In the articles of this issue, we describe molecular characterization of inorganic-binding peptides; explain their further tailoring using post-selection genetic engineering and bioinformatics; and finally demonstrate their utility as molecular synthesizers, erectors, and assemblers. The peptides become fundamental building blocks of functional materials, each uniquely designed for an application in areas ranging from practical engineering to medicine.

show abstract

Section: Prospectsmentioning

confidence: 99%

Molecular Biomimetics: Genetic Synthesis, Assembly, and Formation of Materials Using Peptides

Tamerler¹,

Sarıkaya²

2008

MRS Bull.

View full text Add to dashboard Cite

show abstract

“…58,59 For protein microarrays, each feature on the array must be a binding partner for a protein that might be in the sample. 60 Most commonly, antibodies to the proteins of interest are prepared in high-throughput or semi-high-throughput fashion, and then spotted onto a specially treated surface (eg, glass, silica, etc).…”

Section: Protein Microarraysmentioning

confidence: 99%

Pharmacoproteomics in drug development

Witzmann

Grant

2003

Pharmacogenomics J

View full text Add to dashboard Cite

The field of proteomics is taking on increased significance as the relevance of investigating and understanding protein expression in disease and drug development is appreciated. Recent advances in proteomics have been driven by the availability of numerous annotated whole-genome sequences and a broad range of technological and bioinformatic developments that underscore the complexity of the proteome. This review briefly addresses some of the various technologies that comprise Expression Proteomics and Functional Proteomics, citing examples where these emerging approaches have been applied to pharmacology, toxicology, and the development of drugs.

show abstract

“…The osteointegration of orthopaedic and dental titanium implants can, for example, be improved with coatings of bifunctional peptides selected for their ability to simultaneously bind the titanium alloy and the targeted tissue (Yazici et al ., 2013). Peptide self‐assemblies have also inspired designs of molecular surface coatings used in cell adhesion assays (Chen et al ., 1997) and biosensors (Templin et al ., 2002; Bertone and Snyder, 2005; Ma et al ., 2012; Gupta et al ., 2016). Moreover, peptides can be printed onto solid surfaces in defined array geometries to develop platforms for the screening of antibodies (immunoassays), other proteins and peptides (study of protein–protein interactions), synthetic ligands (drug discovery) or small molecules (protein–metabolite interactions) (Gupta et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

Harnessing the power of microbial nanowires

Reguera

2018

Microbial Biotechnology

View full text Add to dashboard Cite

SummaryThe reduction of iron oxide minerals and uranium in model metal reducers in the genus Geobacter is mediated by conductive pili composed primarily of a structurally divergent pilin peptide that is otherwise recognized, processed and assembled in the inner membrane by a conserved Type IVa pilus apparatus. Electronic coupling among the peptides is promoted upon assembly, allowing the discharge of respiratory electrons at rates that greatly exceed the rates of cellular respiration. Harnessing the unique properties of these conductive appendages and their peptide building blocks in metal bioremediation will require understanding of how the pilins assemble to form a protein nanowire with specialized sites for metal immobilization. Also important are insights into how cells assemble the pili to make an electroactive matrix and grow on electrodes as biofilms that harvest electrical currents from the oxidation of waste organic substrates. Genetic engineering shows promise to modulate the properties of the peptide building blocks, protein nanowires and current‐harvesting biofilms for various applications. This minireview discusses what is known about the pilus material properties and reactions they catalyse and how this information can be harnessed in nanotechnology, bioremediation and bioenergy applications.

show abstract

Protein microarray technology

Cited by 514 publications

References 49 publications

Molecular Biomimetics: Genetic Synthesis, Assembly, and Formation of Materials Using Peptides

Molecular Biomimetics: Genetic Synthesis, Assembly, and Formation of Materials Using Peptides

Pharmacoproteomics in drug development

Harnessing the power of microbial nanowires

Contact Info

Product

Resources

About