Protein Microarrays

Chen, Chien‐Sheng; Zhu, Heng

doi:10.2144/06404te01

Cited by 81 publications

(60 citation statements)

References 24 publications

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“…This approach is complementary to the PBM in terms of the methodology and questions to ask. By definition, a functional protein microarray is formed by immobilizing thousands of individually purified proteins in a single glass slide [37,38]. A functional protein microarray offers a flexible platform that has been applied to characterize many different types of protein activity, including protein-DNA, protein-RNA, protein-lipid, protein-drug, proteinprotein, and protein-antibody interactions [39][40][41][42].…”

Section: Protein Microarraymentioning

confidence: 99%

Systematic characterization of protein-DNA interactions

Xie

Qian

et al. 2011

Cell. Mol. Life Sci.

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Sequence-specific protein-DNA interactions (PDIs) are critical for regulating many cellular processes, including transcription, DNA replication, repair, and rearrangement. We review recent experimental advances in high-throughput technologies designed to characterize PDIs and discuss recent studies that use these tools, including ChIP-chip/seq, SELEX-based approaches, yeast one-hybrid, bacterial one-hybrid, protein binding microarray, and protein microarray. The results of these studies have challenged some long-standing concepts of PDI and provide valuable insights into the complex transcriptional regulatory networks.

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Section: Protein Microarraymentioning

confidence: 99%

Systematic characterization of protein-DNA interactions

Xie

Qian

et al. 2011

Cell. Mol. Life Sci.

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“…Анали-тические служат для выявления белковых профилей в биологических жидкостях и тканях; функциональные используются для изучения биохимических особенно-стей протеома [15,18,19]. Аналитическая чувствитель-ность биочипов примерно в 2 раза выше, чем при имму-нофлюоресцентном и иммуноферментном анализе (ИФА) [20]. Однако их специфичность и аффинность сильно зависят от природы белка: структуры, размера, наличия гидрофобных или гетерофильных боковых це-пей, посттрансляционных модификаций [16].…”

Section: белковые микрочипы (биочипы)unclassified

Proteomic Studies in Rheumatology

Новиков¹,

Александрова²,

Насонов³

2012

rsp

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“…Protein arrays are miniaturized, parallel assay systems that contain small amounts of purified proteins in a highdensity format [38,39]. Protein arrays can be classified into two types, depending on their application: analytical and functional protein arrays.…”

Section: Protein Arraysmentioning

confidence: 99%

Proteomics: addressing the challenges of multiple myeloma

Ge¹,

Tao²,

Bi³

et al. 2011

ABBS

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Multiple myeloma (MM) is a malignancy of terminally differentiated B-lymphocytes that accounts for ∼13% of all hematologic cancers. Despite a wealth of knowledge describing the molecular biology of MM as well as significant advances in therapeutics, this disease remains incurable. Since proteins govern the cellular structure and biological function, a wide selection of proteomic approaches holds great promise for increasing our understanding of this disease, such as by investigating the dynamic nature of protein expression, cellular and subcellular distribution, post-translational modifications, and interactions at both the cellular and subcellular levels. The aims of this review are to introduce the available and emerging proteomic technologies that have potential applications in the study of MM and to highlight the current status of proteomic studies of MM. To date, although there have been a limited number of proteomic studies in MM, those performed have provided valuable information with regard to MM diagnosis and therapy. The potential future application of proteomic technologies is expected to provide new avenues in MM diagnostics, individualized therapy design and therapy response surveillance for the clinician.

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Protein Microarrays

Cited by 81 publications

References 24 publications

Systematic characterization of protein-DNA interactions

Systematic characterization of protein-DNA interactions

Proteomic Studies in Rheumatology

Proteomics: addressing the challenges of multiple myeloma

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