Protein microarrays for diagnostic assays

Hartmann, Michael; Roeraade, Johan; Stoll, Dieter; Templin, Markus F.; Joos, Thomas

doi:10.1007/s00216-008-2379-z

Cited by 153 publications

(111 citation statements)

References 56 publications

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“…For example, many sensing applications rely on the detection of a specific binding event between an immobilized sensor molecule and its binding partner. Analysis of the binding of a patient's serum proteins to an array of antibodies can reveal expression patterns that are diagnostic of disease (Hartmann et al 2009). Sensing of environmental toxins is now possible with sensors based on biomolecular interactions (Frisk et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants

2010

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Plasma modification and plasma polymer deposition are valuable technologies for the preparation of surfaces for the covalent binding of biomolecules for applications such as biosensors, medical prostheses, and diagnostic devices as well as surfaces for enzyme-mediated reactions. Covalency is conveniently tested by the ability of the surface to retain the attached molecules after vigorous washing with sodium dodecyl sulphate (SDS). Covalency is indicated if the fraction of protein retained lies above the curve characteristic of physisorption. Confidence in covalency is strengthened when the washing protocol is aggressive enough to remove all adsorbed protein from a control significantly more hydrophobic than the test surface. The use of linker chemistry to space the molecules from the surface is in some cases beneficial. However, the use of linker chemistry is not necessary to retain molecular function for long periods when the polymer surface is modified by energetic bombardment. The energetic bombardment retains hydrophilicity of the surface by crosslinking the subsurface, and this appears to facilitate retention of protein function. Energetic bombardment also increases the functional life of molecules immobilized and then freeze dried on plasma-modified surfaces. Analysis of the surfaces shows that the covalent binding mechanism is related to the presence of free radicals on the surface and in the subsurface regions. The unpaired electrons associated with the radicals appear to be mobile within the modified region and can diffuse to the surface to take part in binding interactions. Proactive implantable devices can make use of these principles of covalent attachment by seeding the surface of an implant with a biomolecule that elicits the desired interaction with cells and prevents undesirable responses.

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Section: Introductionmentioning

confidence: 99%

Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants

2010

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“…[59][60][61][62][63] To design protein microarrays, we used DP as a protein support because the many carboxylic acid moieties on the periphery can effectively immobilize target proteins. DP-coated surfaces can load more proteins and more protein activity than planar surfaces.…”

Section: Dp-immobilized Surfaces For Diagnostic Toolsmentioning

confidence: 99%

Dendrimer porphyrin-based self-assembled nano-devices for biomedical applications

Jeong

Yoon

Jang

2012

Polym J

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Dendrimers have attracted great interest for the design of functional materials. Because the core porphyrin unit in dendrimer porphyrin (DP) is surrounded by large poly(benzyl ether) dendritic wedges with ionic peripheries, the resulting electrostatic interaction with oppositely charged polymeric materials has been used to create various functional nano-devices. In this paper, we briefly review DP-based self-assembled biomedical nano-devices, including DP-loaded polyion complex micelles, the ternary complex systems used for gene delivery, polymer-metal complex micelles, the hollow nanocapsules used for combination cancer therapy, and the DP-immobilized surfaces used for diagnostic tools.

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“…Protein chips using antibodies as recognition elements are evolving very rapidly [83] . They are not yet as popular as the above -mentioned techniques and formats; this is mainly due to the paucity of suitable antibodies, lack of affi nity, crossreactivity, and loss of functionality upon binding.…”

Section: Antibody Microarraysmentioning

confidence: 99%