Protein Mobilization and Proteolytic Enzyme Activities during Seed Germination of Broad Bean (Vicia faba L.)

Kırmızı, Serap; Guleryuz, Gürcan

doi:10.1515/znc-2006-3-411

Cited by 15 publications

(13 citation statements)

References 21 publications

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“…It is worth mentioning that, germination is generated in a concerted manner that involves the acceleration of respiratory metabolism to produce metabolic energy and biosynthetic precursors (Kırmızı and Gu¨leryu¨z, 2006). So, respiratory carbohydrates, mainly soluble sugars, must be provided frequently to maintain proper germination, enhance defense and act as Fig.…”

Section: Discussionmentioning

confidence: 99%

Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.

Madany

Saleh

2015

Annals of Agricultural Sciences

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Invasive field weeds, such as Euphorbia sp., represent main threat for crop productivity. The present work was conducted to evaluate the phytotoxicity of Euphorbia helioscopia on wheat (Triticum aestivum L.) and pea (Pisum sativum L.). The influence of aqueous extract from shoot of E. helioscopia, at different rates (1.0%, 2.5%, 5.0%, and 10.0%; w/v), on germination and early seedling growth of wheat and pea as well as some of the synchronized physiological aspects was investigated. E. helioscopia aqueous extract severely affected the germination in a concentration dependent manner. Plumule and radicle length, as well as their fresh and dry masses were markedly reduced. Moreover, amylase activity and total soluble sugars were significantly reduced in response to treatment with aqueous Euphorbia extract, in both test plants, whereas, proteolytic activity showed marked improvement. The linear regression analysis revealed the presence of positive linear correlation between germination rate and amylase activity. The stress markers such as proline, phenolics and flavonoids were markedly accumulated upon treatment with E. helioscopia extract. The increment in the level of total phenolics was concomitant with an improvement in phenylalanine ammonia lyase and peroxidase activities. High performance liquid chromatography analysis of E. helioscopia extract revealed the presence of two cinnamic acid derivatives (caffeic and p-coumaric acids), two benzoic acid derivatives (vanillic and syringic acids) and a flavanonol (Dihydroquercetin). To our knowledge, this is the first study to investigate the physiological implications underlying the phytotoxicity of E. helioscopia.ª 2015 Production and hosting by Elsevier B.V. on behalf of Faculty of Agriculture, Ain Shams University.

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Section: Discussionmentioning

confidence: 99%

Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.

Madany

Saleh

2015

Annals of Agricultural Sciences

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“…The high soluble protein content in asphodel tubers could be associated with the continuing proteolytic activities rather than the nitrate assimilation. The amino acids or products of the proteolytic cleavage can be used to meet the metabolic demands of the storage tissue (Kırmızı and Güleryüz, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…The supernatant was collected, and a portion of it was dialysed for 24 h against 50 mM sodium acetate buffer, pH 5.4, containing 10 mM 2-ME. All 3 proteolytic activities were assayed according to the method given by Kırmızı and Güleryüz (2006). L-Leucine 4-nitroanilide was used as the substrate for the LAP activity assays.…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…There is substantial information about the seed proteins and mobilisation of seed proteins by proteinases (Chin et al, 1972;Mitsuhashi et al, 1984;Munoz et al, 1994;Kırmızı and Güleryüz, 2006). However, little information exists about the reserve proteins in the storage organs of geophytes (Guimaráes et al, 2001;Frankova et al, 2006;Kamenetsky et al, 2005).…”

mentioning

confidence: 99%

“…However, little information exists about the reserve proteins in the storage organs of geophytes (Guimaráes et al, 2001;Frankova et al, 2006;Kamenetsky et al, 2005). The storage organs of geophytes also act as sources of nitrogen, sulphur, and carbon (Shewry, 2003), and the proteins can be hydrolysed to amino acids by a number of proteinases (Kırmızı and Güleryüz, 2007). Sakar et al (2010) reported the seasonality of the Asphodelus aestivus nitrate reductase activity (NRA) and the high nitrate assimilation capacity in all plant parts, including flowers and tuberous roots.…”

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confidence: 99%

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Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus

Kırmızı¹,

Guleryuz²,

Arslan³

2014

Turk J Bot

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IntroductionKnowledge of plant traits is necessary for understanding the ecosystem responses to natural and human-induced environmental changes (Hüseyinova et al., 2013). Geophytes are important life forms among plants with specialised structures in specialised environments. Their regenerating buds are on underground storage organs, and their life cycle includes a dormant period that can extend from a few weeks to most of the year, as defined by Raunkiaer (1934). Geophytes are widespread in the Mediterranean part of the world because they can survive the summer drought (Dafni, 1996;Debussche et al., 2004).The Mediterranean geophytes are of 2 types: springflowering species that display leaves and flowers simultaneously (synanthous geophytes), and autumnalflowering species that develop flowers before leaves and leaves after the flowering (hysteranthous geophytes) (Dafni et al., 1981a). The synanthous geophytes have periods of growth, storage, flowering, and dormancy, whereas the hysteranthous geophytes go dormant after storing the food reserves such that vegetative and reproductive stages do not occur at the same time (Dafni et al., 1981a(Dafni et al., , 1981b.According to Pantis (1993), Asphodelus aestivus Brot. could be categorised as intermediate; it is synanthous, but its reproduction depends partly on stored reserves and partly on annual production. Asphodelus aestivus is one of the important geophytes growing in degraded areas of the Mediterranean environment (Pantis et al., 1994). When it covers the degraded parts of the eastern Mediterranean basin, these types of ecosystems appear like deserts, and they are called asphodel geophyte-deserts or asphodel semideserts (Naveh, 1973;Ayyad and Hilmy, 1974;Le Houerou, 1981).Asphodelus aestivus is a competitive ruderal stresstolerant strategist species, which can be found on agricultural slopes, near roads, and in calcareous soils in pastures (Pantis et al., 1994). Asphodelus aestivus is an important plant and it has become the dominant life form in some degraded Mediterranean ecosystems, including those resulting from excessive grazing and fire (Ayyad and Hilmy, 1974;Le Houerou, 1981;Pantis and Margaris, 1988). Pantis and Margaris (1988) reported that the dominance of Asphodelus aestivus in degraded areas reflects both its capacity to face the peculiarities of the Mediterranean climate and its resistance to the most common disturbances in habitat.Geophytes mainly store one or more storage compounds in their underground storage organs. They generally accumulate carbohydrates, starch, soluble sugars, glucomannans, and fructans (

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Monitoring of early‐stage water uptake by hyperspectral imaging and evaluation of nutritional and technological functionality of germinated faba bean (Vicia faba L.) var. minor and var. major as food ingredients

et al. 2021

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Faba bean (Vicia faba L.) is a potential, sustainable protein alternative. Germination behavior of Vicia faba L. var. minor and Vicia faba L. var. major needs further studies in order to enable larger scale bioprocessing. In this study, early-stage water uptake of two distinct faba bean varieties was assessed by hyperspectral imaging. Nutritional and technological functionality of germinated faba bean ingredients as such and in combination with fermentation were evaluated. Hyperspectral imaging revealed that early-stage water uptake in faba beans occurred evenly from the different sides of the beans. Germination on petri dishes and in pilot-scale showed that smaller faba beans moistened and germinated significantly (p < 0.05) faster and retained water better through germination than larger faba beans. Germinated faba flour of minor-type variety resulted in 72% higher dextran production in Weissella confusa VTT E-143403 fermentation than respective native faba flour. With both types of faba bean varieties, germination as such, and with minor-type variety, germination as combined with fermentation decreased notably the content of raffinose family oligosaccharides. These bioprocessing methods also improved functionality of faba bean ingredients by increasing protein separation efficiency in air classification, protein solubility, foaming capacity, and foam stability. Based on this study, minor-and major-type or small-and large-seeded faba bean varieties set different requirements to the germination process. In addition, germination alone or as combined with fermentation was proved to improve the nutritional and technological quality of faba bean material promoting its use in several food applications including also gluten-free products.

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Protein Mobilization and Proteolytic Enzyme Activities during Seed Germination of Broad Bean (Vicia faba L.)

Cited by 15 publications

References 21 publications

Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.

Phytotoxicity of Euphorbia helioscopia L. on Triticum aestivum L. and Pisum sativum L.

Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus

Monitoring of early‐stage water uptake by hyperspectral imaging and evaluation of nutritional and technological functionality of germinated faba bean (Vicia faba L.) var. minor and var. major as food ingredients

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