Protein modifications in Hedgehog signaling

Liu, Min; Su, Ying; Peng, Jingyu; Zhu, Alan Jian

doi:10.1002/bies.202100153

Cited by 7 publications

(3 citation statements)

References 103 publications

(171 reference statements)

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“…Hedgehog (Hh) signaling is a highly conserved pathway that controls multiple developmental processes, including pattern formation, proliferation and differentiation within diverse tissues. Dysfunction of Hh signaling can lead to birth defects, such as holoprosencephaly and cyclopia, and is associated with multiple cancer types, including medulloblastoma and basal cell carcinoma ( Lee et al, 2016 ; Pak and Segal, 2016 ; Jeng et al, 2020 ; Liu M. et al, 2021 ; Jiang, 2021 ; Sigafoos et al, 2021 ). As a classic model system, the Drosophila wing plays a seminal and pivotal role in the delineation of the Hh signaling cascade.…”

Section: Introductionmentioning

confidence: 99%

“…As a result, a cytoplasmic signaling complex containing Cos2, Fu, Su(fu), and transcription factor Cubitus interruptus (Ci) is disassociated. Full-length Ci (Ci FL ) becomes stable and enters the nucleus to activate the expression of downstream target genes, such as decapentaplegic ( dpp ), ptc , and collier ( col ) ( Hartl and Scott, 2014 ; Liu M. et al, 2021 ; Jiang, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Dissection of the microRNA Network Regulating Hedgehog Signaling in Drosophila

Fan

Wang

et al. 2022

Front. Cell Dev. Biol.

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The evolutionarily conserved Hedgehog (Hh) signaling plays a critical role in embryogenesis and adult tissue homeostasis. Aberrant Hh signaling often leads to various forms of developmental anomalies and cancer. Since altered microRNA (miRNA) expression is associated with developmental defects and tumorigenesis, it is not surprising that several miRNAs have been found to regulate Hh signaling. However, these miRNAs are mainly identified through small-scale in vivo screening or in vitro assays. As miRNAs preferentially reduce target gene expression via the 3′ untranslated region, we analyzed the effect of reduced expression of core components of the Hh signaling cascade on downstream signaling activity, and generated a transgenic Drosophila toolbox of in vivo miRNA sensors for core components of Hh signaling, including hh, patched (ptc), smoothened (smo), costal 2 (cos2), fused (fu), Suppressor of fused (Su(fu)), and cubitus interruptus (ci). With these tools in hand, we performed a genome-wide in vivo miRNA overexpression screen in the developing Drosophila wing imaginal disc. Of the twelve miRNAs identified, seven were not previously reported in the in vivo Hh regulatory network. Moreover, these miRNAs may act as general regulators of Hh signaling, as their overexpression disrupts Hh signaling-mediated cyst stem cell maintenance during spermatogenesis. To identify direct targets of these newly discovered miRNAs, we used the miRNA sensor toolbox to show that miR-10 and miR-958 directly target fu and smo, respectively, while the other five miRNAs act through yet-to-be-identified targets other than the seven core components of Hh signaling described above. Importantly, through loss-of-function analysis, we found that endogenous miR-10 and miR-958 target fu and smo, respectively, whereas deletion of the other five miRNAs leads to altered expression of Hh signaling components, suggesting that these seven newly discovered miRNAs regulate Hh signaling in vivo. Given the powerful effects of these miRNAs on Hh signaling, we believe that identifying their bona fide targets of the other five miRNAs will help reveal important new players in the Hh regulatory network.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dissection of the microRNA Network Regulating Hedgehog Signaling in Drosophila

Fan

Wang

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…As a result, a cytoplasmic signaling complex containing Cos2, Fu, Su(fu), and transcription factor Cubitus interruptus (Ci) is disassociated. Full-length Ci (Ci FL ) becomes stable and enters the nucleus to activate the expression of downstream target genes, such as decapentaplegic (dpp), ptc, and collier (col) (Hartl and Scott, 2014;Liu M. et al, 2021;Jiang, 2021). microRNAs (miRNAs) are endogenous noncoding RNAs of approximately 22 nucleotides in length.…”

Section: Introductionmentioning

confidence: 99%

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Cell–cell communication: new insights and clinical implications

Su,

Song,

Zhu

et al. 2024

Sig Transduct Target Ther

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Multicellular organisms are composed of diverse cell types that must coordinate their behaviors through communication. Cell–cell communication (CCC) is essential for growth, development, differentiation, tissue and organ formation, maintenance, and physiological regulation. Cells communicate through direct contact or at a distance using ligand–receptor interactions. So cellular communication encompasses two essential processes: cell signal conduction for generation and intercellular transmission of signals, and cell signal transduction for reception and procession of signals. Deciphering intercellular communication networks is critical for understanding cell differentiation, development, and metabolism. First, we comprehensively review the historical milestones in CCC studies, followed by a detailed description of the mechanisms of signal molecule transmission and the importance of the main signaling pathways they mediate in maintaining biological functions. Then we systematically introduce a series of human diseases caused by abnormalities in cell communication and their progress in clinical applications. Finally, we summarize various methods for monitoring cell interactions, including cell imaging, proximity-based chemical labeling, mechanical force analysis, downstream analysis strategies, and single-cell technologies. These methods aim to illustrate how biological functions depend on these interactions and the complexity of their regulatory signaling pathways to regulate crucial physiological processes, including tissue homeostasis, cell development, and immune responses in diseases. In addition, this review enhances our understanding of the biological processes that occur after cell–cell binding, highlighting its application in discovering new therapeutic targets and biomarkers related to precision medicine. This collective understanding provides a foundation for developing new targeted drugs and personalized treatments.

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Protein modifications in Hedgehog signaling

Cited by 7 publications

References 103 publications

Dissection of the microRNA Network Regulating Hedgehog Signaling in Drosophila

Dissection of the microRNA Network Regulating Hedgehog Signaling in Drosophila

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Cell–cell communication: new insights and clinical implications

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