Protein nanoparticle interaction: A spectrophotometric approach for adsorption kinetics and binding studies

Vaishanav, Sandeep K.; Chandraker, Kumudini; Korram, Jyoti; Nagwanshi, Rekha; Ghosh, Kallol K.; Satnami, Manmohan L.

doi:10.1016/j.molstruc.2016.03.087

Cited by 20 publications

(14 citation statements)

References 73 publications

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Section: Surface Interactions Of Nanomaterials—analytical and Physicomentioning

confidence: 99%

“…105 Such quenching effect of protein fluorescence is often used to study the interaction energetics and thermodynamics with nanoparticles. For example, by measuring the protein fluorescence quenching and employing the Stern-Volmer relation, the binding constant between fibrinogen and carbon nanotubes, 106,107 hemoglobin and AgNPs, 108,109 BSA and lanthanum fluoride-doped terbium nanoparticles 110 or AuNPs 111 were determined. Similarly, emission from fluorescent nanoparticles can also be used to obtain the binding constant.…”

Section: Fluorescence-based Spectroscopiesmentioning

confidence: 99%

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Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

Chen

Riviere

2016

WIREs Nanomed Nanobiotechnol

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The understanding of nano-bio interactions is deemed essential in the design, application, and safe handling of nanomaterials. Proper characterization of the intrinsic physicochemical properties, including their size, surface charge, shape, and functionalization, is needed to consider the fate or impact of nanomaterials in biological and environmental systems. The characterizations of their interactions with surrounding chemical species are often hindered by the complexity of biological or environmental systems, and the drastically different surface physicochemical properties among a large population of nanomaterials. The complexity of these interactions is also due to the diverse ligands of different chemical properties present in most biomacromolecules, and multiple conformations they can assume at different conditions to minimize their conformational free energy. Often these interactions are collectively determined by multiple physical or chemical forces, including electrostatic forces, hydrogen bonding, and hydrophobic forces, and calls for multidimensional characterization strategies, both experimentally and computationally. Through these characterizations, the understanding of the roles surface physicochemical properties of nanomaterials and their surface interactions with biomacromolecules can play in their applications in biomedical and environmental fields can be obtained. To quantitatively decipher these physicochemical surface interactions, computational methods, including physical, statistical, and pharmacokinetic models, can be used for either analyses of large amounts of experimental characterization data, or theoretical prediction of the interactions, and consequent biological behavior in the body after administration. These computational methods include molecular dynamics simulation, structure-activity relationship models such as biological surface adsorption index, and physiologically-based pharmacokinetic models. WIREs Nanomed Nanobiotechnol 2017, 9:e1440. doi: 10.1002/wnan.1440 For further resources related to this article, please visit the WIREs website.

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Section: Surface Interactions Of Nanomaterials—analytical and Physicomentioning

confidence: 99%

Section: Fluorescence-based Spectroscopiesmentioning

confidence: 99%

Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

Chen

Riviere

2016

WIREs Nanomed Nanobiotechnol

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“…61 Finally, Vaishanav et al used fluorescence spectroscopy to investigate protein/GNPs interactions. 62 Based on these studies, we used fluorescence spectroscopy to understand the interaction between GNPs and LYZ or BSA proteins in aqueous solution. After an excitation with a 250 nm radiation, the fluorescence spectra of BSA and lysozyme solutions, at a fixed concentration of 10 mg/mL, were recorded after addition of increasing volumes of a GNP solution.…”

Section: Results and Discussionmentioning

confidence: 99%

Assembly of Multicomponent Nano-Bioconjugates Composed of Mesoporous Silica Nanoparticles, Proteins, and Gold Nanoparticles

et al. 2019

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The purpose of this work was the assembly of multicomponent nano-bioconjugates based on mesoporous silica nanoparticles (MSNs), proteins (bovine serum albumin, BSA, or lysozyme, LYZ), and gold nanoparticles (GNPs). These nano-bioconjugates may find applications in nanomedicine as theranostic devices. Indeed, MSNs can act as drug carriers, proteins stabilize MSNs within the bloodstream, or may have therapeutic or targeting functions. Finally, GNPs can either be used as contrast agents for imaging or for photothermal therapy. Here, amino-functionalized MSNs (MSN–NH 2 ) were synthesized and characterized through various techniques (small angle X-rays scattering TEM, N 2 adsorption/desorption isotherms, and thermogravimetric analysis (TGA)). BSA or lysozyme were then grafted on the external surface of MSN–NH 2 to obtain MSN–BSA and MSN–LYZ bioconjugates, respectively. Protein immobilization on MSNs surface was confirmed by Fourier transform infrared spectroscopy, ζ-potential measurements, and TGA, which also allowed the estimation of protein loading. The MSN–protein samples were then dispersed in a GNP solution to obtain MSN–protein–GNPs nano-bioconjugates. Transmission electron microscopy (TEM) analysis showed the occurrence of GNPs on the MSN–protein surface, whereas almost no GNPs occurred in the protein-free control samples. Fluorescence and Raman spectroscopies suggested that proteins–GNP interactions involve tryptophan residues.

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“…Therefore, UV–vis absorption spectroscopy is also generally used together with other spectroscopic methods in interfacial analyses. [ 82 ]…”

Section: Generally Used Spectroscopic Methods For Np Probingmentioning

confidence: 99%

In Situ Spectroscopic Probes for Structures and Processes at the Surface of Noble Metallic Nanoparticles

Xue

Guha

Yuan

et al. 2021

Part & Part Syst Charact

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Protein nanoparticle interaction: A spectrophotometric approach for adsorption kinetics and binding studies

Cited by 20 publications

References 73 publications

Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

Biological and environmental surface interactions of nanomaterials: characterization, modeling, and prediction

Assembly of Multicomponent Nano-Bioconjugates Composed of Mesoporous Silica Nanoparticles, Proteins, and Gold Nanoparticles

In Situ Spectroscopic Probes for Structures and Processes at the Surface of Noble Metallic Nanoparticles

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