Protein-nanoparticle interactions

Lynch, Iseult; Dawson, Kenneth A.

doi:10.1016/s1748-0132(08)70014-8

Cited by 1,602 publications

(1,230 citation statements)

References 44 publications

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“…1 H relaxometry was used to measure longitudinal, T 1 , and transverse, T 2 , relaxation times over a wide range of frequencies including 8.5, 21, and 63 MHz representative of clinical MRI systems. For frequencies in the range of 10 kHz < f < 10 MHz, Fig.…”

Section: Magnetic Properties Relaxivity and Mri Contrast Efficiencymentioning

confidence: 99%

Protein corona affects the relaxivity and MRI contrast efficiency of magnetic nanoparticles

et al. 2013

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Magnetic nanoparticles (NPs) are increasingly being considered for use in biomedical applications such as biosensors, imaging contrast agents and drug delivery vehicles. In a biological fluid, proteins associate in a preferential manner with NPs. The small sizes and high curvature angles of NPs influence the types and amounts of proteins present on their surfaces. This differential display of proteins bound to the surface of NPs can influence the tissue distribution, cellular uptake and biological effects of NPs. To date, the effects of adsorption of a protein corona (PC) on the magnetic properties of NPs have not been considered, despite the fact that some of their potential applications require their use in human blood.Here, to investigate the effects of a PC (using fetal bovine serum) on the MRI contrast efficiency of superparamagnetic iron oxide NPs (SPIONs), we have synthesized two series of SPIONs with variation in the thickness and functional groups (i.e. surface charges) of the dextran surface coating. We have observed that different physico-chemical characteristics of the dextran coatings on the SPIONs lead to the formation of PCs of different compositions. 1 H relaxometry was used to obtain the longitudinal, r 1 , and transverse, r 2 , relaxivities of the SPIONs without and with a PC, as a function of the Larmor frequency. The transverse relaxivity, which determines the efficiency of negative contrast agents (CAs), is very much dependent on the functional group and the surface charge of the SPIONs' coating. The presence of the PC did not alter the relaxivity of plain SPIONs, while it slightly increased the relaxivity of the negatively charged SPIONs and dramatically decreased the relaxivity of the positively charged ones, which was coupled with particle agglomeration in the presence of the proteins. To confirm the effect of the PC on the MRI contrast efficiency, in vitro MRI experiments at n ¼ 8.5 MHz were performed using a low-field MRI scanner. The MRI contrasts, produced by different samples, were fully in agreement with the relaxometry findings.It is well-recognized that the surface of nanoparticles (NPs) is covered by biomolecules (proteins, sugars and lipids) upon coming into contact with biological systems, resulting in the formation of a protein "corona" that is strongly associated with the NPs' surface and that denes how living organisms (e.g. cells) "see" the NPs in a biological milieu. 1-5 According to previous reports, the cell "sees" a nano-system in which the NPs are covered by a "hard" corona of slowly exchanging proteins with a surrounding "so" corona consisting of weakly interacting and rapidly exchanging proteins. 6 The composition of the protein corona (PC) is highly dependent on the physicochemical properties of the NPs such as their size, composition, and surface characteristics. 7 Superparamagnetic iron oxide nanoparticles (SPIONs) have been recognized as very promising nanosystems with good biocompatibility 8,9 and high potential to be used for diagnosis and therapy simultaneou...

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Section: Magnetic Properties Relaxivity and Mri Contrast Efficiencymentioning

confidence: 99%

Protein corona affects the relaxivity and MRI contrast efficiency of magnetic nanoparticles

et al. 2013

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“…The scheme in polymeric NPs with and without oleic acid [34]. Very different interaction patterns were observed in the presence and absence of oleic acid.…”

Section: Discussionmentioning

confidence: 95%

Surface modification of HSA containing magnetic PLGA nanoparticles by poloxamer to decrease plasma protein adsorption

Shubhra

Tóth

Gyenis

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Lifetime prolongation for hydrophobic drug carriers has been the focus of interest for many years. Poloxamer (Pluronic F68, PF68) has been employed in this study for modifying the surface of magnetic poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with human serum albumin (HSA) model drug. Surface characteristics of untreated and PF68 treated NPs were analyzed by size, zeta potential and electrophoretic mobility studies. UV/VIS spectroscopic analysis, isothermal titration calorimetry (ITC) and dynamic light scattering methods were used to investigate serum protein (bovine serum albumin, BSA) adsorption. Results showed the successful surface attachment of PF68. Among different concentrations (0.1 to 1% wt/vol) of PF68 studied, 0.5% was found to be the most useful, since a higher concentration can issue in micelle formation. 50% less BSA tended to be adsorbed on the treated NPs in comparison to the untreated ones.

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“…When MNPs get inside of the human body, they come into contact with different biomolecules, especially protein (Bihari et al 2008;Lynch and Dawson 2008), which lead to altered properties of MNPs, thereby affect their biodistribution and interactions with biostructures and cells. The binding of protein with MNPs can trigger conformational changes in protein folding, altering its biological function, and affecting the signaling pathways activated by MNPs (Bihari et al 2008;Lynch and Dawson 2008).…”

Section: Mnps' Health Effect By Life Cyclementioning

confidence: 99%

Nanoparticle pollution and associated increasing potential risks on environment and human health: a case study of China

Gao

Yang

Jin³

2015

Environ Sci Pollut Res

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The aims of this study are (1) to discuss the mechanism of nanoparticle lifecycle and estimate the impacts of its associated pollution on environment and human health; and (2) to provide recommendation to policy makers on how to leverage nanopollution and human health along with the rapid development of economics in China. Manufactured nanoparticles (MNPs) could either directly or indirectly impair human health and the environment. Exposures to MNP include many ways, such as via inhalation, ingestion, direct contact, or the use of consumer products over the lifecycle of the product. In China, the number of people exposed to MNP has been increasing year by year. To better provide medical care to people exposed to MNP, the Chinese government has established many disease control and prevention centers over China. However, the existing facilities and resources for controlling MNP are still not enough considering the number of people impacted by MNP and the number of ordinary workers in the MNP related industry applying for their occupational identification through the Center for Disease Control and Prevention. China should assess the apparent risk environment and human health being exposed to MNP and develop action plans to reduce the possibility of direct contacts between human beings and the emerging nanomaterials. In addition, we suggest more comprehensive studies on the MNP behavior and the development of quantitative approaches to measure MNP transport, and persistence should be carried out.

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Protein-nanoparticle interactions

Cited by 1,602 publications

References 44 publications

Protein corona affects the relaxivity and MRI contrast efficiency of magnetic nanoparticles

Protein corona affects the relaxivity and MRI contrast efficiency of magnetic nanoparticles

Surface modification of HSA containing magnetic PLGA nanoparticles by poloxamer to decrease plasma protein adsorption

Nanoparticle pollution and associated increasing potential risks on environment and human health: a case study of China

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