“…Homobifunctional Nhydroxysuccinimide (NHS)-ester functionalized polyethylene glycol (PEG) based macromers were incorporated into the jetting solution with the protein. [91] Specifically, 2 kDa O,O′bis[2-(N-succinimidyl-succinylamino)ethyl]polyethylene glycol (PEG-NHS) and 4,7,10, 13,16,19,22,25,32,35,38,41,44,47,50,53hexadecaoxa-28,29-dithiahexapentacontanedioic acid di-Nsuccinimidyl ester (PEG-NHS-S) were used that reacted with the proteins' amine groups. [91] The reaction was completed after incubating the solidified PNPs at 37 °C for seven days.…”