Protein Nutrition and the Biochemical Composition of the Uterus

Leathem, James H.; Oslapas, Raymond; Fisher, Cynthia J.

doi:10.1016/s0015-0282(16)36606-7

Cited by 8 publications

(5 citation statements)

References 9 publications

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“…The pattern of the reduced ALP in tissues reflects on the lack of endogenous ovarian hormones due to ovariectomy, which is known to significantly reduce ALP activity [57]. The increase in ALP activity after estradiol treatment is in agreement with the earlier studies [58]. Antagonistic action of MTX in combination with steroids is confirmed in our studies by the decrease in ALP activity.…”

Section: Alkaline Phosphatase (Alp)supporting

confidence: 92%

“…ALP is an important enzyme that maintains the structure and function of female genital tract [1,56,57]. Variations in ALP reflect the secretory patterns of endogenous ovarian hormones during menstrual cycle [56][57][58][59][60]. Studies revealed that OVX caused a significant reduction in ALP activity [57].…”

Section: Estrogen and Progesteronementioning

confidence: 99%

“…Antagonistic action of MTX in combination with steroids is confirmed in our studies by the decrease in ALP activity. In rats there are conflicting reports 404 claiming an increase [59], no change or a decrease [58] in ALP activity following estradiol administration. This decrease in ALP activity in the current study may be due to reduced facilitation of the transport of amino acids into the tissue cells ultimately leading to a decrease in protein synthesis.…”

Section: Alkaline Phosphatase (Alp)mentioning

confidence: 99%

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Impact of Methotrexate and Leucovorin on Hormonal Regulated Enzymes of Carbohydrate Metabolism in Accessory Reproductive Tissues of Ovariectomized Rats

Karri¹

2012

Transl Med

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Background: Female reproductive tract cells are fast proliferating like cancer cells. Methotrexate (MTX), an anticancer drug when used to treat non-neoplastic diseases in young women results in deleterious effects leading to infertility. Oviduct, uterus, cervix and vagina depend on ovary for their structural and functional sustenance. Ovarian ablation followed by chemotherapy in premenopausal women is a common practice in the treatment of cancer. Hence, methotrexate effect on reproductive organs in Ovariectomized (OVX) rats was investigated. Objective: To reveal the direct influence of MTX on estradiol and progesterone in OVX rats. Also, the protective role of leucovorin (LCN) an antidote to MTX, estradiol (E219) and progesterone (P) replacement was investigated. Methods: Animals were randomly divided into the following groups (n=6): group 1: OVX, group 2: OVX+MTX, group 3: OVX+MTX+LCN, group 4: OVX+E2, OVX+MTX+LCN+E222, group 5: OVX+P, group 6: OVX+MTX+LCN+P, treated once/day intramuscularly (im) for 4 days and sacrificed on day 5. At the end of the experiment reproductive 24 tract tissues oviduct, uterus, cervix and vagina were used for total protein, glycogen, Glucose-6-Phosphate26 Dehydrogenase (G6PD), Lactate Dehydrogenase (LDH) and Alkaline Phosphatase (ALP) enzyme activities. Results: MTX treatment reduced protein, glycogen, G6PD, LDH and ALP activities in oviduct, uterus, cervix and vagina of OVX rats. However, LCN supplementation was partially protective on some enzymes, which was tissue dependent. Hormonal replacement improved protein and enzyme levels in all the tissues. Estradiol treatment showed a distinct enhancement over progesterone in elevating such levels. However, MTX and LCN in a combination treatment with steroids reversed the activities of enzymes in all the tissues. Conclusion: The results suggest that MTX severely affects protein, glycogen, G6PD, LDH and ALP levels in accessory reproductive organs of OVX rats. These studies conclude that in premenopausal women ovarian ablation and chemotherapy affect the accessory reproductive tissues, consequently distressing their quality of life.

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Section: Alkaline Phosphatase (Alp)supporting

confidence: 92%

Section: Estrogen and Progesteronementioning

confidence: 99%

Section: Alkaline Phosphatase (Alp)mentioning

confidence: 99%

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Impact of Methotrexate and Leucovorin on Hormonal Regulated Enzymes of Carbohydrate Metabolism in Accessory Reproductive Tissues of Ovariectomized Rats

Karri¹

2012

Transl Med

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“…he March 1968 issue of Fertility and Sterility contains the article, ''Protein nutrition and the biochemical composition of the uterus'' (1). In this article, Leathem et al (1) discuss the correlation between undernutrition, decreased fertility, increased menstrual irregularities and reduction in estrogen levels. The etiology was believed to be a reduction in ovarian function with subsequent downstream effect on the uterus.…”

mentioning

confidence: 99%

Uterine architecture: building the foundation for a healthy pregnancy

Feinberg

2018

Fertility and Sterility

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“…In pregnant rats, likewise, omission of methionine (11) or tryptophane (17) from the diet leads to reproductive failure, and the addition of 4% methionine to a 20% casein diet results in subnormal fetal and placental weights (18). In this investigation a different approach to the study of reproductive function has been utilized-that of supplementing the protein-free diet with a single crystalline amino acid in an attempt to maintain pregnancy.…”

mentioning

confidence: 99%

Maintenance of Pregnancy in Protein-Deficient Rats with Dietary Supplements of Methionine

Kinzey

1970

Experimental Biology and Medicine

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Protein Nutrition and the Biochemical Composition of the Uterus

Cited by 8 publications

References 9 publications

Impact of Methotrexate and Leucovorin on Hormonal Regulated Enzymes of Carbohydrate Metabolism in Accessory Reproductive Tissues of Ovariectomized Rats

Impact of Methotrexate and Leucovorin on Hormonal Regulated Enzymes of Carbohydrate Metabolism in Accessory Reproductive Tissues of Ovariectomized Rats

Uterine architecture: building the foundation for a healthy pregnancy

Maintenance of Pregnancy in Protein-Deficient Rats with Dietary Supplements of Methionine

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