Protein O-mannosylation affects protein secretion, cell wall integrity and morphogenesis in Trichoderma reesei

Maize Antifungal Protein AFP1 Elevates Fungal Chitin Levels by Targeting Chitin Deacetylases and Other Glycoproteins

“…PMTs form a heterodimer or homodimer in Saccharomyces cerevisiae ( 48 ). Loss of pmt4 significantly impacts the O -mannosylation of membrane proteins to which cell surface hydrophobicity and cell wall integrity defects may be attributed ( 49 – 52 ). U. maydis PMTs may form a dimer complex to mannosylate their UmCDA targets that bear a Ser-Thr stretch before the GPI anchor, which is missing in UmCda4.…”

Structural, Evolutionary, and Functional Analysis of the Protein O-Mannosyltransferase Family in Pathogenic Fungi

“…The Pmt protein family is widely conserved as its members are crucial for many biological functions. In pathogenic fungi, Pmts are key to virulence due to their roles in maintaining cell wall integrity and secretion of fungal effectors [ 14 , 16 , 37 , 38 , 39 , 40 ]. In plant pathogenic fungi, the Pmt family consists of three members: Pmt1, Pmt2, and Pmt4.…”

“…Pmt4 might thus be the Pmt protein that O-mannosylates more virulence-related glycoproteins than the other Pmts do. In this context, it has been observed in Fusarium oxysporum and Trichoderma reesei that Pmt4 specifically O-mannosylates membrane proteins [ 39 , 41 ] and soluble proteins in the secretory pathways, particularly, GPI-anchored proteins. By contrast, Pmt2 is more related to substrates implicated in cell wall synthesis [ 41 ].…”

Repurposing the cellulase workhorse Trichoderma reesei as a ROBUST chassis for efficient terpene production

“…22,23 T. reesei has sophisticated endomembrane structures and subcellular compartments, a powerful ability for protein synthesis, and reasonable ability for protein glycosylation modification, which is advantageous for the heterologous expression of enzymes in the synthetic pathway of target metabolites. 24 As the workhorse for cellulase production, reliable and economical industrial-scale fermentation processes have already been established. 25 Therefore, T. reesei possesses the physical and molecular components required for the heterologous biosynthesis of natural products.…”