Protein–Oligonucleotide Interactions

The transcription factor c-Myb is involved in early differentiation and proliferation of haematopoietic cells, where it operates as a regulator of self-renewal and multi-lineage differentiation. Deregulated c-Myb plays critical roles in leukaemias and other human cancers. Due to its role as a master regulator, we hypothesized it might function as a pioneer transcription factor. Our approach to test this was to analyse a mutant of c-Myb, D152V, previously reported to cause haematopoietic defects in mice by an unknown mechanism. Our transcriptome data from K562 cells indicates that this mutation specifically affects c-Myb's ability to regulate genes involved in differentiation, causing failure in c-Myb's ability to block differentiation. Furthermore, we see a major effect of this mutation in assays where chromatin opening is involved. We show that each repeat in the minimal DNA-binding domain of c-Myb binds to histones and that D152V disrupts histone binding of the third repeat. ATAC-seq data indicates this mutation impairs the ability of c-Myb to cause chromatin opening at specific sites. Taken together, our findings support that c-Myb acts as a pioneer factor and show that D152V impairs this function. The D152V mutant is the first mutant of a transcription factor specifically destroying pioneer factor function.

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“…DNA binding was monitored by the electrophoretic mobility shift assay, including titration and off-rate experiments, as described in ( 39 , 40 ).…”

Section: Methodsmentioning

confidence: 99%

A c-Myb mutant causes deregulated differentiation due to impaired histone binding and abrogated pioneer factor function

Fuglerud

Lemma

Wanichawan

et al. 2017

Nucleic Acids Research

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“…Sequence-specific DNA-binding was examined by electrophoretic mobility shift assay (EMSA) as previously described [55]. The binding reactions were performed in 20 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 10% glycerol, 0.1 mM DTT, 0.005% Triton X-100 and 50 mM NaCl in a total volume of 20 μl.…”

Section: Methodsmentioning

confidence: 99%

Selective inhibition of c-Myb DNA-binding by RNA polymers

2004

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BackgroundThe transcription factor c-Myb is expressed in hematopoietic progenitor cells and other rapidly proliferating tissues, regulating genes important for proliferation, differentiation and survival. The DNA-binding domain (DBD) of c-Myb contains three tandemly arranged imperfect repeats, designated Myb domain R1, R2 and R3. The three-dimensional structure of the DBD shows that only the second and third Myb domains are directly involved in sequence-specific DNA-binding, while the R1 repeat does not contact DNA and only marginally affects DNA-binding properties. No structural information is available on the N-terminal 30 residues. Since deletion of the N-terminal region including R1 plays an important role in oncogenic activation of c-Myb, we asked whether this region confers properties beyond DNA-binding to the neighbouring c-Myb DBD.ResultsAnalysis of a putative RNA-binding function of c-Myb DBD revealed that poly(G) preferentially inhibited c-Myb DNA-binding. A strong sequence-selectivity was observed when different RNA polymers were compared. Most interesting, the poly(G) sensitivity was significantly larger for a protein containing the N-terminus and the R1-repeat than for the minimal DNA-binding domain.ConclusionPreferential inhibition of c-Myb DNA binding by poly(G) RNA suggests that c-Myb is able to interact with RNA in a sequence-selective manner. While R2 and R3, but not R1, are necessary for DNA-binding, R1 seems to have a distinct role in enhancing the RNA-sensitivity of c-Myb.

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Protein–Oligonucleotide Interactions

Cited by 2 publications

References 98 publications

A c-Myb mutant causes deregulated differentiation due to impaired histone binding and abrogated pioneer factor function

A c-Myb mutant causes deregulated differentiation due to impaired histone binding and abrogated pioneer factor function

Selective inhibition of c-Myb DNA-binding by RNA polymers

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