Protein oxidation and turnover

Chang, Tsu‐Chung; Chou, Wei-Yuan; Chang, Gu‐Gang

doi:10.1007/bf02255811

Cited by 23 publications

(8 citation statements)

References 82 publications

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“…The down-regulation of proteins involved in oxidative stress response (Cta1, Ccp1) might be directly correlated with the lower abundance of proteins involved in fatty acid metabolism (Faa2 and Cat2) and respiratory proteins as many basic steps in energy metabolism involve transition metal ions and the generation of toxic oxygen species. 40 Components of the protein folding and secretion machinery were also down-regulated at lower cultivation temperature. Chaperones Ssa4 and Ssb1 showed strongly reduced abundances, whereas two protein spots identified as fragments of these two heat shock proteins with a molecular weight of approximately 25 kDa showed significantly higher levels at 20 °C.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Temperature on the Proteome of Recombinant Pichia pastoris

et al. 2009

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The impact of environmental factors on the productivity of yeast cells is poorly investigated so far. Therefore, it is a major concern to improve the understanding of cellular physiology of microbial protein production hosts, including the methylotrophic yeast Pichia pastoris. Two-Dimensional Fluorescence Difference Gel electrophoresis and protein identification via mass spectrometry were applied to analyze the impact of cultivation temperature on the physiology of a heterologous protein secreting P. pastoris strain. Furthermore, specific productivity was monitored and fluxes through the central carbon metabolism were calculated. Chemostat culture conditions were applied to assess the adaption to different growth temperatures (20, 25, 30 degrees C) at steady-state conditions. Many important cellular processes, including the central carbon metabolism, stress response and protein folding are affected by changing the growth temperature. A 3-fold increased specific productivity at lower cultivation temperature for an antibody Fab fragment was accompanied by a reduced flux through the TCA-cycle, reduced levels of proteins involved in oxidative stress response and lower cellular levels of molecular chaperones. These data indicate that folding stress is generally decreased at lower cultivation temperatures, enabling more efficient heterologous protein secretion in P. pastoris host cells.

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Section: Resultsmentioning

confidence: 99%

The Effect of Temperature on the Proteome of Recombinant Pichia pastoris

et al. 2009

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“…Accumulation of modified nonfunctional proteins or "aged proteins", contributing to the occurrence of the so called age-related diseases [63], has been suggested to be the consequence of impairment in the proteasome system. In the present study, the proteasome activator complex subunit 1 increased in adults, but decreased in cells from old donors, and this is consistent with the hypothesis of a less efficient proteasome system during senescence [64].…”

Section: Aging and Protein Degradationmentioning

confidence: 99%

Proteome analysis of dermal fibroblasts cultured in vitro from human healthy subjects of different ages

et al. 2003

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Aging is a complex multifactorial process still far from being completely understood. The aim of the present study was to compare the proteome of in vitro cultured dermal fibroblasts from healthy subjects of different ages (i.e. 15 +/- 2, 41 +/- 4 and 82 +/- 3 years old). Proteins of the cell layer were separated by two-dimensional electrophoresis and protein identification was performed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry; moreover, synthetic gels were qualitatively and quantitatively analyzed by Melanie 3 software. Our study did not reveal any protein typical of any one age group. On the other hand, we observed 38 proteins exhibiting more than three-fold reproducible variations with aging, some (45%) being reduced such as F-actin capping protein alpha1, proteasome subunit alpha type 3, heat shock protein 27, ubiquitin carboxyl-terminal hydrolase isozyme L1, mitochondrial thioredoxin-dependent peroxide reductase, cathepsin B, glutathione S-transferase P, cyclophilin A and calgizzarin. In contrast, T-complex protein 1, probable protein disulfide isomerase ER60, phosphoglycerate kinase 1, Ran-specific GTPase-activating protein, proteasome subunit alpha type 5, triosephosphate isomerase and superoxide dismutase (Mn) increased with age. Furthermore, annexin 1, elongation factor 1beta, proteasome activator complex subunit 1, phosphoglycerate mutase, superoxide dismutase (Cu-Zn) and cofilin, exhibited the highest levels in adult cells; whereas, septin 2 homolog, RNA-binding protein regulatory subunit and ATP synthase D chain revealed the lowest values in adults. The present investigation, underlining the complexity of the aging process, highlights the role of synthetic and degradative pathways in modulating the whole cell machinery and emphasizes that metabolic impairment with age could depend partly on different expression of a number of genes and leading to an imbalance among functional proteins.

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“…Both enzymatic and non-enzymatic scavenging antioxidants are the first line defense to combat ROS and inhibit the formation of oxidative lesions [22]. Once lesions formed, the second line of defensive system is to fix the lesions (mostly oxidized DNA) via repairing mechanisms [23][24][25][26] or to degrade them (mostly oxidized proteins) through proteasome and turnover mechanism [27][28][29]. The signaling pathway that regulates cytoprotective response to ROS is the Nrf2 (nuclear factor erythroid 2 [NF-E2]-related factor 2)-Keap1 (Kelch-like ECH-associated protein 1) pathway [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress in Urolithiasis

Boonla¹

2018

Reactive Oxygen Species (ROS) in Living Cells

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Oxygen is absolutely essential for the survival of our life. However, metabolic consumption of oxygen inevitably yields reactive oxygen species (ROS). Imbalance of ROS production and antioxidant capacity causes oxidative stress that potentially damages biomolecules leading to cell injury and death. In fact, ROS have two-faceted functions. Under physiologic condition, ROS function as signaling molecules and participate in maintaining redox balance. In pathology, ROS induce oxidative stress that critically involves in the development of several diseases including urolithiasis (UL). UL or urinary stone disease is a common urologic condition in all countries with progressively increasing prevalence. Most of UL are multifactorial with polygenic susceptibility and highly recurrent nature. Formation of urinary stones is driven by supersaturation of urinary lithogenic ions, and calcium oxalate (CaOx) is the most prevalent stone type. Oxidative stress clearly plays an active role in UL development. In vitro, lithogenic crystals induce ROS generation in renal tubular cells leading to oxidative stress, cell injury and release of inflammatory mediators. In nephrolithic rats, oxidative stress and CaOx deposit are gradually increased in the rats' kidneys. Intervention with antioxidants efficiently reduces oxidative damage and crystal deposits. Human studies show that patients with UL have increased oxidative stress and renal tubular injury relative to the non-stone-forming individuals. Increased oxidative lesions and inflammation are observed in the stone-containing kidneys of the patients. Furthermore, renal fibrosis mediated through tubular epithelial-mesenchymal transition is observed in kidneys of stone patients. Increased renal fibrosis is significantly associated with decreased kidney function. From therapeutic point of view, nutraceutical regimens that are able to reduce oxidative stress may be clinically useful alternatives for preventing stone formation and recurrence. This chapter has an intention to provide a basic knowledge of ROS generation and oxidative stress and up-to-date research findings of oxidative stress in UL based on the published articles as well as the author's studies.

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Protein oxidation and turnover

Cited by 23 publications

References 82 publications

The Effect of Temperature on the Proteome of Recombinant Pichia pastoris

The Effect of Temperature on the Proteome of Recombinant Pichia pastoris

Proteome analysis of dermal fibroblasts cultured in vitro from human healthy subjects of different ages

Oxidative Stress in Urolithiasis

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