Protein Oxidative Stress and Dyslipidemia in Dialysis Patients

Abstract

Our aim was to investigate and determine the associations between oxidative stress (OS), dyslipidemia and inflammation in patients treated with continuous ambulatory peritoneal dialysis (CAPD) and hemodialysis (HD) using observational cross-sectional study. Twenty patients in CAPD and 48 in HD for at least 8 weeks and aged ≥18 years were included in the study. Individuals with malignant or acute inflammatory disease were excluded. A control group of 17 healthy individuals was also recruited. The biochemical parameter evaluations were analyzed using colorimetric kits for albumin, serum glucose, total cholesterol (TC) and lipid fractions. To determine the inflammatory status, CRP, IL-6 and TNF-α were analyzed by automated chemiluminescence kits. Plasma advanced oxidation protein products (AOPP) were determined by spectrophotometry. Mean AOPP levels were significantly higher for the HD group compared to the control, and there was no difference in AOPP concentrations between the control and CAPD groups. Dialysis patients had levels of inflammatory parameters higher than controls, and showed a high prevalence of patients with dyslipidemia, especially in CAPD. In the HD group, AOPP was positively correlated with triglycerides (TG) and inversely associated with HDL. Also the HD group was observed to have negative associations between TNF-α and HDL, LDL and TC. In the CAPD group, CRP was inversely correlated with HDL. Hemodialysis patients had increased protein OS and associations of inflammation and dyslipidemia were also observed in these dialysis groups. A more detailed characterization of the relations between oxidative stress and other more traditional risk factors has therapeutic importance, since cardiovascular diseases are the leading cause of death among dialysis patients.

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

“…The disulfide/native thiol levels were also different between the three groups; the disulfide/native thiol levels were slightly higher in the transplantation and PD groups compared to the HD group. While these results are consistent with few other previous studies, increased levels of oxidative stress in HD compared to those in PD have also been reported in some studies (20)(21)(22)(23)(24). We believe that the increased oxidative stress observed in the PD patients (compared to those in the HD patients) in our study may have been caused by inflammatory responses.…”

Oxidative Dna Damage and Mortality in Hemodialysis and Peritoneal Dialysis Patients

“…Also, it is not known whether oxidative stress varies with dialysis modality. In a few studies, increased levels of oxidative stress have been reported to be associated with hemodialysis (HD) compared with peritoneal dialysis (PD) (18,19), but other studies showed the opposite results (20)(21)(22).…”

Aspectos oxidativos da síndrome metabólica e aterosclerose

“…Esta é uma das razões pelas quais há níveis anormais de lipídeos na resistência à insulina. Elevados níveis de lipoproteína de baixa densidade ligada ao colesterol (LDL-c) e baixos níveis de HDL-c, além de aumentos nos níveis de proteína C reativa, também se relacionam com os radicais livres em diferentes estudos [29][30][31][32][33].…”

“…Observa-se elevação de marcadores de estresse oxidativo em modelos animais quando há níveis elevados de LDL-c e baixos níveis de HDL-c e, em seres humanos, observa-se elevação destes marcadores quando há hipertrigliceridemia e baixos níveis de HDL-c. Medicações hipocolesterolemiantes, como algumas estatinas, podem reduzir o estresse oxidativo porque aumentam a síntese de enzimas antioxidantes, como glutationa peroxidase, catalase e superóxido dismutase [3,29,31,35].…”