Protein-phospholipid-cholesterol interaction in the photolysis of invertebrate rhodopsin

Kusumi, Akihiro; Tsuda, Motoyuki; Akino, Toyoaki; Ohnishi, Shun‐ichi; Terayama, Yoshio

doi:10.1021/bi00274a027

Cited by 49 publications

(33 citation statements)

References 32 publications

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“…The role of cholesterol in the plasma membrane is a long-standing puzzle. It has been proposed that the mixture of saturated and unsaturated alkyl chains with cholesterol provides a fluidity buffer [5,61]. Present results suggest that cholesterol has several important functions in the plasma membrane, specific to this membrane.…”

Section: Hydrophobic Barrier Of Lipid Bilayer Membranes Formed By Alkmentioning

confidence: 59%

Physical properties of lipid bilayer membranes: relevance to membrane biological functions.

Subczynski¹,

Wiśniewska²

2000

Acta Biochim Pol

107

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Over the last 25 years one of us (WKS) has been investigating physical properties of lipid bilayer membranes. In 1991 a group led by WKS was organized into the Laboratory of Structure and Dynamics of Biological Membranes, the effective member of which is AW. Using mainly the electron paramagnetic resonance (EPR) spin-labeling method, we obtained unexpected results, which are significant for the better understanding of the functioning of biological membranes. We have developed a new pulse EPR spin-labeling method for the detection of membrane domains and evaluation of lipid exchange rates. This review will be focused on our main results which can be summarized as follows: (1) Unsaturation of alkyl chains greatly reduces the ordering and rigidifying effects of cholesterol although the unsaturation alone gives only minor fluidizing effects, as observed by order and reorientational motion, and rather significant rigidifying effects, as observed by translational motion of probe molecules; (2) Abbreviations: EPR, electron paramagnetic resonance; PC, phosphatidylcholine; DXPC (where X = L, M, P, S, B, O, E), dilauroyl-, dimyristoyl-, dipalmitoyl-, distearoyl-, dibehenoyl-, dioleoyl-, dielaidoylphosphatidylcholine, respectively; EYPC, egg-yolk phosphatidylcholine; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; BR, bacteriorhodopsin; IFV, influenza virus; SASL, stearic acid spin label; 16-SASL, 16-doxylstearic acid spin label; SLOT domain, slow oxygen transport domain; DOT method, method of discrimination by oxygen transport; T 1, spin-lattice relaxation time.Here, the word "transport" is used in its basic physical sense indicating the product of the (local) translational diffusion coefficient and the (local) concentration of oxygen in the membrane.

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Section: Hydrophobic Barrier Of Lipid Bilayer Membranes Formed By Alkmentioning

confidence: 59%

Physical properties of lipid bilayer membranes: relevance to membrane biological functions.

Subczynski¹,

Wiśniewska²

2000

Acta Biochim Pol

107

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“…Kusumi et al [96] first introduced the expression “fluidity buffer,” stating that “cholesterol acts as a ‘fluidity buffer’ so that small changes in the amount and arrangement of membrane proteins may not affect the overall properties of the membranes.” Later, he and the co-authors proposed that the membrane mixture of saturated and unsaturated acyl chains of PLs with Chol also provides a fluidity buffer [97, 98]. Present results suggest that Chol also has other crucial functions specific to certain biological membranes.…”

Section: Discussionmentioning

confidence: 99%

High Cholesterol/Low Cholesterol: Effects in Biological Membranes: A Review

Subczynski

Pasenkiewicz-Gierula

Widomska

et al. 2017

Cell Biochem Biophys

239

160

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Lipid composition determines membrane properties, and cholesterol plays a major role in this determination as it regulates membrane fluidity and permeability as well as induces the formation of coexisting phases and domains in the membrane. Biological membranes display a very diverse lipid composition, the lateral organization of which plays a crucial role in regulating a variety of membrane functions. We hypothesize that, during biological evolution, membranes with a particular cholesterol content were selected to perform certain functions in the cells of eukaryotic organisms. In this review, we discuss the major membrane properties induced by cholesterol, and their relationship to certain membrane functions.

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“…Cholesterol (Chol) constitutes up to 50% of the lipids in the plasma membranes of eukaryotic cells (Sackmann, 1995). The biological roles of Chol involve the maintenance of proper fluidity (e.g., Kusumi et al, 1983), reduction of passive permeability (e.g., Subczynski et al, 1994), and increasing the mechanical strength and elasticity (e.g., Table 1. Average values (ranges) of parameters that characterise liquid-crystalline PC bilayers.…”

Section: Hydrated Lipid Bilayers Containing Cholesterolmentioning

confidence: 99%

Molecular dynamics simulation studies of lipid bilayer systems.

et al. 2000

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The main structural element of biological membranes is a liquid-crystalline lipid bilayer. Other constituents, i.e. proteins, sterols and peptides, either intercalate into or loosely attach to the bilayer. We applied a molecular dynamics simulation method to study membrane systems at various levels of compositional complexity. The studies were started from simple lipid bilayers containing a single type phosphatidylcholine (PC) and water molecules (PC bilayers). As a next step, cholesterol (Chol) molecules were introduced to the PC bilayers (PC-Chol bilayers). These studies provided detailed information about the structure and dynamics of the membrane/water interface and the hydrocarbon chain region in bilayers built of various types of PCs and Chol. This enabled studies of membrane systems of higher complexity. They included the investigation of an integral membrane protein in its natural environment of a PC bilayer, and the antibacterial activity of magainin-2. The latter study required the construction of a model bacterial membrane which consisted of two types of phospholipids and counter ions. Whenever published experimental data were available, the results of the simulations were compared with them.

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Protein-phospholipid-cholesterol interaction in the photolysis of invertebrate rhodopsin

Cited by 49 publications

References 32 publications

Physical properties of lipid bilayer membranes: relevance to membrane biological functions.

Physical properties of lipid bilayer membranes: relevance to membrane biological functions.

High Cholesterol/Low Cholesterol: Effects in Biological Membranes: A Review

Molecular dynamics simulation studies of lipid bilayer systems.

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