Protein precipitation by polyethylene glycol: A generalized model based on hydrodynamic radius

Capture and intermediate purification of recombinant antibodies with combined precipitation methods

“…The precipitation of proteins with PEG occurs in a volume exclusion reaction [16,17]. In recent years, further research was performed to determine the solubility curve of common proteins during the precipitation process [18][19][20][21][22][23]. Precipitation by PEG does not allow removal of high molecular weight impurities (HMWIs), like DNA [24] or aggregates, because the precipitation of larger proteins tends to require lower PEG concentrations and shorter http://dx.doi.org/10.1016/j.bej.2014.…”

Drug Target Interference in Immunogenicity Assays: Recommendations and Mitigation Strategies

“…PEGs are highly hydrophilic and are known to attract water molecules away from the solvation layer around the protein, causing protein precipitation. The effectiveness of PEG precipitation greatly depends on the hydrodynamic radius of the protein [31]. In a recent report, a monomeric drug target dimerized at acidic pH during the acid dissociation step of the bridging assay, leading to false-positive signal [28].…”

Developing the biofacility of the future based on continuous processing and single-use technology

“…Consequently, alternative UO for the capture step or intermediate purification/polishing like aqueous two phase extraction (Muendges et al, 2015;Rosa et al, 2011;Schwienheer et al, 2015), precipitation (Sim et al, 2012;Hammerschmidt et al, 2014) or crystallization (Schmidt et al, 2005) have not been taken into account. Although, these methods might have advantages regarding continuous operation mode and process economics, they do not fulfill the requirement of being a platform technology in antibody production.…”