Protein production for human use from sugarbeet: Byproducts

Jwanny, Etidal W.; Montanari, Laura; Fantozzi, Paolo

doi:10.1016/0960-8524(93)90085-p

Cited by 17 publications

(11 citation statements)

References 10 publications

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“…Mangold and lucerne not only had the highest N yields in this study, but also the highest N content in the white protein isolate (S4), with values of 4.0% and 3.6%, respectively (Table 2c). Higher N content has been reported in other studies fractionating white protein from sugarbeet, e.g., 9.6% and 7.6% [16,28], and from lucerne, e.g., 11.2%, 10.7%, 11.8%, and 14.8% [11,15,24,37]. As noted above for N yield, comparisons between studies are difficult, although a product mostly corresponding to fraction P3 in this study is often used as an end-point in other studies.…”

Section: Effect Of Biomass Source On Protein Fractionationmentioning

confidence: 44%

“…To enable direct comparisons of different green leaf sources during protein fractionation, the same protein fractionation procedure was applied for all crops. The methodology selected was mainly based on literature data [18,24,28,31], although with some modifications. In principle, the fractionation procedure comprised three steps; screw pressing, thermal precipitation, and acid precipitation (see Sections 2.2.1-2.2.3).…”

Section: Protein Fractionation From Green Leavesmentioning

confidence: 99%

“…In a Nordic context, biomass availability will clearly vary between seasons and a biorefinery would benefit from having multiple raw materials available. However, previous studies have mainly focused on green biomass of three crops (lucerne, sugarbeet, and spinach) for protein fractionation [11,13,[15][16][17][18]21,[23][24][25][26][27][28][29][30][31][32], with only a few studies focusing on other biomass sources (e.g., beetroot, broccoli, cauliflower, or cabbage leaves [33]). Thus, there is limited information available on how different biomass sources perform as raw material in protein extraction and how the extraction process may need to be adapted for individual sources.…”

Section: Introductionmentioning

confidence: 99%

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Protein Fractionation of Green Leaves as an Underutilized Food Source—Protein Yield and the Effect of Process Parameters

Nynäs

Newson

Johansson

2021

Foods

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Green biomass has potential as a sustainable protein source for human consumption, due to its abundance and favorable properties of its main protein, RuBisCO. Here, protein fractionation outcomes of green leafy biomass from nine crops were evaluated using a standard protocol with three major steps: juicing, thermal precipitation, and acid precipitation. Successful protein fractionation, with a freeze-dried, resolubilized white protein isolate containing RuBisCO as the final fraction, was achieved for seven of the crops, although the amount and quality of the resulting fractions differed considerably between crops. Biomass structure was negatively correlated with successful fractionation of proteins from biomass to green juice. The proteins in carrot and cabbage leaves were strongly associated with particles in the green juice, resulting in unsuccessful fractionation. Differences in thermal stability were correlated with relatedness of the biomass types, e.g., Beta vulgaris varieties showed similar performance in thermal precipitation. The optimal pH values identified for acid precipitation of soluble leaf proteins were lower than the theoretical value for RuBisCO for all biomass types, but with clear differences between biomass types. These findings reveal the challenges in using one standard fractionation protocol for production of food proteins from all types of green biomass and indicate that a general fractionation procedure where parameters are easily adjusted based on biomass type should instead be developed.

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Section: Effect Of Biomass Source On Protein Fractionationmentioning

confidence: 44%

Section: Protein Fractionation From Green Leavesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protein Fractionation of Green Leaves as an Underutilized Food Source—Protein Yield and the Effect of Process Parameters

Nynäs

Newson

Johansson

2021

Foods

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“…Experiments show that proteins can be extracted and fractionated from sugar beet tops by using a wet process to obtain two types of leaf protein concentrates. One, obtained by heat coagulation of the green juice with protein contents of approximately 34%, and another, obtained from the brown juice with protein contents of 44%, in both cases the results depend on the temperature and time used for extraction (Jwanny et al, 1993). Another method applied is to utilize enzymes to facilitate the process of extracting protein (Akyüz and Ersus, 2021).…”

Section: Bio-products: Extraction Of Wax Furfural and Proteinmentioning

confidence: 99%

Biogas Technology as an “Engine” for Facilitating Circular Bio-Economy in Denmark—The Case of Lolland & Falster Municipalities Within Region Zealand

Lybæk

Kjær

2021

Front. Energy Res.

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This article investigates how biogas technology can facilitate the deployment of municipal circular bio-economic solutions within the energy and agrarian sectors in Denmark. The emphasis is on the regional climate policy and the existing biogas technology concepts, within a decentralized energy market located in the Southern part of Zealand. The case analysis will identify how such technology can be utilized as a lever for future “extraction-activities,” as for example protein, wax, and furfural substrates. Within Falster & Lolland Municipalities, it is identified that 800.000 tons of animal manure is readily available for biogas production, just as 880.000 tons and 220.000 tons of unused beet tops and residual cereal straw could be feed to biogas facilities as for example co-silage materials. With a potential gas yield of approximately 897.000 MWh, composed by the crop residues alone, the challenge is how to utilize such resources the most efficient when addressing future needs for bio-products and high value materials and energy. Through the lens of Circular Bio-Economy this article addresses three themes, by which biogas technology can become an “engine” for future bioenergy solutions, where cascading activities and use of side-streams are developed: 1) production of biogas by means of local agricultural residues (beet tops, residual straw, and animal manure), combined with 2) “extraction-activities” as furfural and wax from straw, as well as protein from beet tops. Besides this 3) opportunities for upgrading the biogas and distributing it on a natural gas network, hereby enlarging the supply market for energy services from the biogas plant and facilitating the development of a more “integrated energy system,” currently being promoted by the European Commission. This article concludes on a step-by-step approach to utilize biomass residues more efficiently in light of the CBE concept and cascading approach, and the available biomass resources within the specific case area addressed.

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“…Leaves are good feedstock for bioconversion processes. In addition, the protein in leaves can be extracted as a valuable byproduct for food and feed applications [6] [7] [8].…”

Section: Introductionmentioning

confidence: 99%

Response Surface Optimization of Enzymatic Hydrolysis of Sugar Beet Leaves into Fermentable Sugars for Bioethanol Production

Aramrueang¹,

Zicari²,

Zhang³

2017

ABB

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Sugar beet leaves are the major crop waste from sugar beet production, while the unused leaves contain a high number of sugars and polysaccharides. The effects of different enzyme products (cellulase, Cellic CTec2; xylanase, Cellic HTec2; and pectinase, Pectinex Ultra SPL) were determined during high-solids enzymatic hydrolysis of sugar beet leaves at 10% total solids (TS) content. Response surface methodology was used to study the effects of enzyme loadings during the hydrolysis of sugar beet leaves for producing fermentable sugars. It was found that both cellulases and pectinases are important enzymes for the hydrolysis of sugar beet leaves. Enzyme loading and reaction time were important factors. Based on the amount of sugars released, a maximum sugar conversion of 82% was achieved after 72 h of hydrolysis using 30 filter paper unit (FPU) g −1 glucan for cellulase and 150 polygalacturonase unit (PGU) g −1 polygalacturonic acid for pectinase, or 37 FPU g −1 glucan for cellulase and 100 PGU g −1 polygalacturonic acid for pectinase. The corresponding sugar yield and sugar concentration were 0.35 g•g −1 TS, and 35 g•l −1 , respectively. Sugar conversion ranged from 59% -70%, 68% -80%, and 74% -82% after 24 h, 48 h, and 72 h of hydrolysis depending on the design conditions.

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Protein production for human use from sugarbeet: Byproducts

Cited by 17 publications

References 10 publications

Protein Fractionation of Green Leaves as an Underutilized Food Source—Protein Yield and the Effect of Process Parameters

Protein Fractionation of Green Leaves as an Underutilized Food Source—Protein Yield and the Effect of Process Parameters

Biogas Technology as an “Engine” for Facilitating Circular Bio-Economy in Denmark—The Case of Lolland & Falster Municipalities Within Region Zealand

Response Surface Optimization of Enzymatic Hydrolysis of Sugar Beet Leaves into Fermentable Sugars for Bioethanol Production

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