Protein Production inStaphylococcus carnosusand other Gram‐Positive Bacteria

Abstract

Originally published in: Production of Recombinant Proteins. Edited by Gerd Gellissen. Copyright © 2005 Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3‐527‐31036‐4 The sections in this article are Introduction Major Protein Export Routes in Gram‐positive Bacteria The General Secretion (Sec) Pathway The Twin‐Arginine Translocation (Tat) Pathway Secretion Signals Extracytosolic Protein Folding The Cell Wall as a Barrier for the Secretion of Heterologous Proteins Degradation of Exported Proteins by Cell‐associated and Secreted Proteases Staphylococcus carnosus General Description Microbiological and Molecular Biological Tools S. carnosus as Host Organism for the Analysis of Staphylococcal‐related Pathogenicity Aspects Secretory Production of Heterologous Proteins by S. carnosus The Staphylococcus hyicus Lipase: Secretory Signals and Heterologous Expression in S. carnosus Use of the Pre‐pro‐part of the S. hyicus Lipase for the Secretion of Heterologous Proteins in S. carnosus Process Development for the Secretory Production of a Human Calcitonin Precursor Fusion Protein by S. carnosus Surface Display on S. carnosus Appendix