Protein profile of Dabry’s sturgeon (Acipenser dabryanus) spermatozoa and relationship to sperm quality

Li, Ping; Du, Hao; Qiao, Xin Mei; Liu, Zhi Gang; Zhou, Qiong; Qi, Wei

doi:10.1016/j.anireprosci.2018.12.002

Cited by 7 publications

(2 citation statements)

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“…The male and female reproductive organs germinate, mature, and degenerate in different proglottids (immature, mature, and gravid) (25). Some proteins related to fertilization were upregulated in adult worms, such as dihydrolipoyl dehydrogenase and ropporin-1-like, which are responsible for sperm capacitation, and CCT-beta and calnexin, which are associated with sperm-egg recognition (26,27). Furthermore, a set of proteins related to male and female gamete generation was upregulated in the PSC of E. granulosus, such as LIM and SH3 domain proteins, which are associated with male gamete generation (28), and some important vaccine candidate antigens, such as major egg antigen, antigen EG13, FABP2, 14-3-3, and tetraspanin.…”

Section: Discussionmentioning

confidence: 99%

Global profiling of the proteome, phosphoproteome, and N-glycoproteome of protoscoleces and adult worms of Echinococcus granulosus

Wang,

Jia,

et al. 2023

Front. Vet. Sci.

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IntroductionCystic echinococcosis (CE) is a chronic zoonosis caused by infection with the metacestode of the Echinococcus granulosus. A unique characteristic of E. granulosus protoscolex (PSC) is their ability to develop bidirectionally into an adult worm in the definitive host or a secondary hydatid cyst in the intermediate host. Furthermore, cestodes have a complex life cycle involving different developmental stages; however, the mechanisms underlying this development remain unknown. Several studies have demonstrated that certain matrix proteins undergo posttranslational modifications (PTMs), including phosphorylation and glycosylation, which have important regulatory effects on their functional properties.MethodsSystematic analyses of the proteome, phosphorylated modified proteome, and glycosylated modified proteome of protoscoleces (PSCs) and adult worms were performed using a proteomic strategy. Data are available via ProteomeXchange with identifier PXD043166.ResultsIn total, 6,407 phosphorylation sites and 1757 proteins were quantified. Of these, 2032 phosphorylation sites and 770 proteins were upregulated, and 2,993 phosphorylation sites and 1,217 proteins were downregulated in adult worms compared to PSCs. A total of 612 N-glycosylation sites were identified in the 392 N-glycoproteins. Of these, 355 N-glycosylation sites and 212 N-glycoproteins were quantified. Of these, 90 N-glycosylation sites and 64 N-glycoproteins were upregulated, and 171 N-glycosylation sites and 126 N-glycoproteins were downregulated in adult worms compared to PSCs. GO enrichment analysis indicated that the differentially expressed phosphoproteins were mainly enriched in the regulation of oxidoreduction coenzyme metabolic processes, myelin sheath, and RNA helicase activity, whereas the differentially expressed N-glycoproteins were enriched in the cellular response to unfolded proteins, endoplasmic reticulum lumen, and nucleic acid binding. KEGG enrichment analysis indicated that the differently expressed phosphoproteins were mainly enriched in RNA transport, hypertrophic cardiomyopathy (HCM), glycolysis/gluconeogenesis, HIF-1 signaling pathway and pyruvate metabolism. Differentially expressed N-glycoproteins were enriched in the PI3K-Akt signaling pathway, ECM-receptor interactions, and protein processing in the endoplasmic reticulum.DiscussionTo our knowledge, this study is the first global phosphoproteomic and N-glycoproteomic analysis of E. granulosus, which provides valuable information on the expression characteristics of E. granulosus and provides a new perspective to elucidate the role of protein phosphorylation and N-glycosylation in the development of E. granulosus.

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Section: Discussionmentioning

confidence: 99%

Global profiling of the proteome, phosphoproteome, and N-glycoproteome of protoscoleces and adult worms of Echinococcus granulosus

Wang,

Jia,

et al. 2023

Front. Vet. Sci.

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“…2018), Dabry's sturgeon ( Acipenser dabryanus) (Li et al . 2019) and common carp (Li et al . 2010b; Dietrich et al .…”

Section: Protein Damagementioning

confidence: 99%

Oxidative stress and use of antioxidants in fish semen cryopreservation

Sandoval‐Vargas

Jiménez

González

et al. 2020

Reviews in Aquaculture

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Reactive oxygen species (ROS) have been proposed as one of the main causes of the impairment of fish spermatozoa integrity and functionality during cryopreservation. The high content of unsaturated fatty acids in sperm cells and the low antioxidant capacity of diluted semen are key factors in making sperm cells susceptible to ROS attacks. For this reason, some recent studies have determined the antioxidant status of the seminal plasma and spermatozoa of fish species. Additionally, some studies have evaluated the effects of antioxidants on post-thaw sperm quality. Although ROS are certainly involved in sperm damage, other factors, such as ice crystal formation, seem to play a crucial role in cryodamage. This challenge has not yet been resolved because both the endogenous antioxidant capacity of the semen and its response to different supplementation practices seem to present specific inter-and intraspecies characteristics and effects. This review summarises knowledge on antioxidant defence and oxidative stress in fish semen, as well as antioxidant supplementation in cryopreservation media, in order to establish perspectives for future studies.

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Shotcrete based 3D concrete printing: State of art, challenges, and opportunities

Heidarnezhad

Zhang

2022

Construction and Building Materials

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Protein profile of Dabry’s sturgeon (Acipenser dabryanus) spermatozoa and relationship to sperm quality

Cited by 7 publications

References 61 publications

Global profiling of the proteome, phosphoproteome, and N-glycoproteome of protoscoleces and adult worms of Echinococcus granulosus

Global profiling of the proteome, phosphoproteome, and N-glycoproteome of protoscoleces and adult worms of Echinococcus granulosus

Oxidative stress and use of antioxidants in fish semen cryopreservation

Shotcrete based 3D concrete printing: State of art, challenges, and opportunities

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