Protein profile of MCF-7 breast cancer cell line treated with lectin delivered by CaCO3NPs revealed changes in molecular chaperones, cytoskeleton, and membrane-associated proteins

Mahmood, Rana I.; Abbass, Amal Kh.; Razali, Nuguelis; Al-Saffar, Ali Z.; Al‐Obaidi, Jameel R.

doi:10.1016/j.ijbiomac.2021.06.144

Cited by 12 publications

(10 citation statements)

References 84 publications

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“…Agaricus bisporus, also known as common mushroom, button mushroom, champignon mushroom, or portobello mushroom, is one of the most commonly and widely consumed mushrooms in the world. Mahmood et al [228] studied the antitumor effects of a lectin isolated from A. bisporus (ABL-Agaricus bisporus lectin) conjugated with CaCO 3 nanoparticles (ABL-CaCO 3 NPs) on MCF-7 breast cancer cells. Fungal lectins are known to have significant pharmacological potential, which includes antioxidant, antitumor, and immunostimulating properties [229].…”

Section: Agaricus Bisporusmentioning

confidence: 99%

“…Ingenuity pathway analysis (IPA) revealed that the upregulated proteins hornerin (HRNR), tropomyosin alpha-1 chain (TPM1), annexin A (ANXA2), and protein-disulfide isomerase (PDI) were associated with breast or gynecological cancer, breast carcinoma, and inflammation of organ. Breast cancer-related protein hornerin (HRNR), part of S100 protein family that is involved in malignant transformation, cell proliferation, differentiation, and death, and known to be elevated in some cancers, was not significantly altered in the ABL-CaCO 3 NPs group, but was increased in DOX group, which could be an indicator of poor prognosis [228,232,233]. From the molecular chaperones/heat shock protein group, protein disulfide isomerase (PDI) was downregulated in ABL-CaCO 3 NPs-treated group with respect to negative control and upregulated in comparison with DOX.…”

Section: Agaricus Bisporusmentioning

confidence: 99%

“…From the molecular chaperones/heat shock protein group, protein disulfide isomerase (PDI) was downregulated in ABL-CaCO 3 NPs-treated group with respect to negative control and upregulated in comparison with DOX. PDI is known to be involved in the unfolded protein response (UPR) pathway and is correlated with worse outcome in breast and other carcinomas, so its downregulation could enhance apoptosis in MCF-7 cells [228,234,235]. Binding immunoglobulin protein (BiP), also known as glucoseregulated protein 78 (GRP78), is a protein from the same molecular chaperone group.…”

Section: Agaricus Bisporusmentioning

confidence: 99%

“…From the cytoskeleton and associated proteins group, both actin citoplasmic 1 or beta-actin (ACTB) and tropomyosin alpha 1 chain and alpha-4 chain (TPM1 and TPM4) were downregulated. Abnormal abundance of ACTB is associated with cancer invasiveness and metastasis, and its high abundance in DOX-treated cells and low abundance in ABL-CaCO 3 NPs can potentially inhibit Erk1/2 and decrease cycline A and decelerate proliferation and anti-apoptotic responses [228]. TPM1 and TPM4, which were downregulated in comparison with both controls, are known to be differentially abundant in breast cancer epithelial vs. normal breast tissues [237].…”

Section: Agaricus Bisporusmentioning

confidence: 99%

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Proteomic Research on the Antitumor Properties of Medicinal Mushrooms

Jakopović¹,

Oršolić

Jakopovich³

2021

Molecules

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Medicinal mushrooms are increasingly being recognized as an important therapeutic modality in complementary oncology. Until now, more than 800 mushroom species have been known to possess significant pharmacological properties, of which antitumor and immunomodulatory properties have been the most researched. Besides a number of medicinal mushroom preparations being used as dietary supplements and nutraceuticals, several isolates from mushrooms have been used as official antitumor drugs in clinical settings for several decades. Various proteomic approaches allow for the identification of a large number of differentially regulated proteins serendipitously, thereby providing an important platform for a discovery of new potential therapeutic targets and approaches as well as biomarkers of malignant disease. This review is focused on the current state of proteomic research into antitumor mechanisms of some of the most researched medicinal mushroom species, including Phellinus linteus, Ganoderma lucidum, Auricularia auricula, Agrocybe aegerita, Grifola frondosa, and Lentinus edodes, as whole body extracts or various isolates, as well as of complex extract mixtures.

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Section: Agaricus Bisporusmentioning

confidence: 99%

Section: Agaricus Bisporusmentioning

confidence: 99%

Section: Agaricus Bisporusmentioning

confidence: 99%

Section: Agaricus Bisporusmentioning

confidence: 99%

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Proteomic Research on the Antitumor Properties of Medicinal Mushrooms

Jakopović¹,

Oršolić

Jakopovich³

2021

Molecules

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“…Over the last 20 years, MS-based quantitative proteomics has progressed at a tremendous rate for biomarker development and drug screening. Furthermore, proteomics offers a large number of protein expression profiles that use quantitative techniques to predict and create relationships through bioinformatics tools [16]. Current quantitative differences between samples with and without drug therapy, or between normal and abnormal cells, are useful in determining proteins with upregulation or downregulation and for establishing biologic processes and pathways [17].…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS Proteomic Study of MCF-7 Cell Treated with Dox and Dox-Loaded Calcium Carbonate Nanoparticles Revealed Changes in Proteins Related to Glycolysis, Actin Signalling, and Energy Metabolism

Ahmed

Ajat

Mahmood

et al. 2021

Biology

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One of the most prevalent death causes among women worldwide is breast cancer. This study aimed to characterise and differentiate the proteomics profiles of breast cancer cell lines treated with Doxorubicin (DOX) and Doxorubicin-CaCO3-nanoparticles (DOX-Ar-CC-NPs). This study determines the therapeutic potential of doxorubicin-loaded aragonite CaCO3 nanoparticles using a Liquid Chromatography/Mass Spectrometry analysis. In total, 334 proteins were expressed in DOX-Ar-CC-NPs treated cells, while DOX treatment expressed only 54 proteins. Out of the 334 proteins expressed in DOX-CC-NPs treated cells, only 36 proteins showed changes in abundance, while in DOX treated cells, only 7 out of 54 proteins were differentially expressed. Most of the 30 identified proteins that are differentially expressed in DOX-CC-NPs treated cells are key enzymes that have an important role in the metabolism of carbohydrates as well as energy, including: pyruvate kinase, ATP synthase, enolase, glyceraldehyde-3-phosphate dehydrogenase, mitochondrial ADP/ATP carrier, and trypsin. Other identified proteins are structural proteins which included; Keratin, α- and β-tubulin, actin, and actinin. Additionally, one of the heat shock proteins was identified, which is Hsp90; other proteins include Annexins and Human epididymis protein 4. While the proteins identified in DOX-treated cells were tubulin alpha-1B chain and a beta chain, actin cytoplasmic 1, annexin A2, IF rod domain-containing protein, and 78 kDa glucose-regulated protein. Bioinformatics analysis revealed the predicted canonical pathways linking the signalling of the actin cytoskeleton, ILK, VEGF, BAG2, integrin and paxillin, as well as glycolysis. This research indicates that proteomic analysis is an effective technique for proteins expression associated with chemotherapy drugs on cancer tumours; this method provides the opportunity to identify treatment targets for MCF-7 cancer cells, and a liquid chromatography-mass spectrometry (LC-MS/MS) system allowed the detection of a larger number of proteins than 2-DE gel analysis, as well as proteins with maximum pIs and high molecular weight.

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