Protein profiling of adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] genotypes grown under inorganic, organic and Zero Budget Natural Farming

Abstract

Fifteen genotypes of adzuki bean grown under inorganic, organic and Zero Budget Natural Farming (ZBNF) condition of Himachal Pradesh were analyzed for protein banding pattern on polyacrylamide gel through SDS-PAGE. The organic, inorganic and ZBNF banding pattern showed significant variability in molecular weight and its ranged from size 11.8 kDa -139.5 kDa, 11.7 kDa -131.5 kDa and 10.6 kDa -96.9 kDa, respectively. The protein banding profiles of adzuki bean genotypes was scored and subjected to cluster analysis using Unweighted Pair Group Method and Arithmetic Mean (UPGMA) for dendrogram grouping. The dendrogram generated from the SDS-PAGE profiles grouped the inorganic, organic and ZBNF genotypes into two major clusters i.e. Cluster-1 (genotypes grown under inorganic systems) and Cluster-2 (genotypes grown under organic and ZBNF systems). The present study demonstrates that genotypes grown under ZBNF system exhibited some distinct protein bands of low molecular weight (10.6 kDa, belong to 11S and 2S globulins than genotypes grown under inorganic and organic system. Hence, the biochemical characterization revealed more specific discrimination among the genotypes grown under inorganic, organic and ZBNF system.